In vitro allergy tests compared to intradermal testing in horses with recurrent airway obstruction

Tahon, L.; Baselgia, S.; Gerber, V.; Doherr, Marcus G.; Straub, R.; Robinson, N. E.; Marti, Eliane

doi:10.1016/j.vetimm.2008.09.021

Cited by 49 publications

(48 citation statements)

References 34 publications

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“…Therefore it is always essential to run mouse IgE positive controls alongside the experiment (Figure 1), and to maintain a large frozen stock of secreting cells. Other research work, such as 20 have indicated that equine RAO is not related to IgE mediation, which is why some discrepancy can be seen with other publishers such as Moran et al 31 when using total equine serum to test to Ca 2+ influx from RAO suffering horses using unspecific reaginic antibodies, for which different antibodies would be responsible for the cellular Ca 2+ influx.…”

Section: Discussionmentioning

confidence: 92%

Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI

Sabban

Helm

2014

JoVE

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Citation: Sabban, S., Ye, H., Helm, B. Development of an in vitro model system for studying the interaction of Equus caballus IgE with its highaffinity receptor FcεRI. J. Vis. Exp. (93), e52222, doi:10.3791/52222 (2014). AbstractThe interaction of IgE with its high-affinity Fc receptor (FcεRI) followed by an antigenic challenge is the principal pathway in IgE mediated allergic reactions. As a consequence of the high affinity binding between IgE and FcεRI, along with the continuous production of IgE by B cells, allergies usually persist throughout life, with currently no permanent cure available. Horses, especially race horses, which are commonly inbred, are a species of mammals that are very prone to the development of hypersensitivity responses, which can seriously affect their performance. Physiological responses to allergic sensitization in horses mirror that observed in humans and dogs. In this paper we describe the development of an in situ assay system for the quantitative assessment of the release of mediators of the allergic response pertaining to the equine system. To this end, the gene encoding equine FcεRIα was transfected into and expressed onto the surface of parental Rat Basophil Leukemia (RBL-2H3.1) cells. The gene product of the transfected equine α-chain formed a functional receptor complex with the endogenous rat β-and γ-chains 1 . The resultant assay system facilitated an assessment of the quantity of mediator secreted from equine FcεRIα transfected RBL-2H3.1 cells following sensitization with equine IgE and antigenic challenge using β-hexosaminidase release as a readout 2,3 . Mediator release peaked at 36.68% ± 4.88% at 100 ng ml -1 of antigen. This assay was modified from previous assays used to study human and canine allergic responses 4,5 . We have also shown that this type of assay system has multiple applications for the development of diagnostic tools and the safety assessment of potential therapeutic intervention strategies in allergic disease 6,2,3 . Video LinkThe video component of this article can be found at

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Section: Discussionmentioning

confidence: 92%

Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI

Sabban

Helm

2014

JoVE

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“…In general, airway inflammation involves the activation of pathogenic-specific inflammatory cells, modulation of transcription factors and release of inflammatory mediators (Barnes, 1998). Type I hypersensitivity, which is IgE-mediated (Halliwell et al, 1993;Schmallenbach et al, 1998;Eder et al, 2000Eder et al, , 2001Curik et al, 2003;Kunzle et al, 2007;Tahon et al, 2009;Moran et al, 2010a,b), and type III hypersensitivity reactions have been suggested to play a role in airway inflammation (Franchini et al, 2000;Lavoie et al, 2001;. Studies suggest that RAO-affected horses have higher IgE levels against Faenia rectivirgula and Aspergillus fumigatus extracts in BALF (bronchoalveolar lavage fluid) than healthy controls (Halliwell et al, 1993;Schmallenbach et al, 1998).…”

Section: The Immunological Basis Of Raomentioning

confidence: 99%

Recurrent airway obstruction in horses - an allergic inflammation: a review

Morán¹,

Folch²

2011

Vet. Med.

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Recurrent airway obstruction (RAO), also known as heaves, is a debilitating and incurable disease of the equine airway. Affected horses develop bronchoconstriction and neutrophilic airway inflammation as a result of exposure to specific airborne irritants and allergens such as hay mould and barn dust. Clinical signs of RAO include exercise intolerance, coughing, nostril flare and abdominal push related to respiratory effort. Evidence suggests that both the innate and acquired immune response contribute to the activation of inflammatory cells resulting in type I hypersensitivity and type III hypersensitivity reactions with an increased expression of Th1-and Th2-cytokines, chemokines, adhesion molecules, receptors and release of reactive oxygen species (ROS). The regulation of inflammatory gene expression in RAO-affected horses is dependent on the binding of transcription factors such as Nuclear factor-(kappa)B (NF-kB), activator protein-1 (AP-1) and the cyclic AMP response element binding protein (CREB) to the promoter region of target genes. In chronic disease an increased number of mucous-producing cells and increased amounts of stored mucins are observed in conjunction with other characteristics of airway tissue remodelling. In this review the findings related to the inflammatory and immunologic response in RAO-affected horses will be presented, and this information will be integrated into existing concepts of immunopathologic mechanisms. Keywords: RAO; horses; cytokines; immune response; airway inflammationList of abbreviations AP-1 = activator protein-1; BALF = bronchoalveolar lavage fluid; CREB = cyclic AMP response element binding prote; eCLCA1 = calcium-activated chloride channels; NF-kB = nuclear factor-(kappa)B; RAO = recurrent airway obstruction; ROS = reactive oxygen species; TLRs = Toll-like receptors

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“…In general, airway inflammation in RAO-affected horses involves the activation of pathogen specific inflammatory cells, modulation of gene transcription factors and the release of inflammatory mediators (Bureau et al 2000 a,b ). Type I hypersensitivity, which is IgE-mediated , Schmallenbach et al 1998, Eder et al 2000, Eder et al 2001, Curik et al 2003, Künzle et al 2007, Tahon et al 2009, Moran et al 2010, and type III hypersensitivity reactions have been suggested to play a role in airway inflammation in RAO-affected horses (Franchini et al 2000, Lavoie et al 2001.…”

Section: Introductionmentioning

confidence: 99%

“…However, although the immunological aspects of RAO have been extensively studied, the precise sequence of events is not fully understood (Leguillette 2003, Wagner 2009). In addition, these investigations have been hampered by problems with the use of antibodies for the detection of equine IgE (Tahon et al 2009) and the lack of suitable immunological tools. A rodent model with controlled environmental conditions would be useful and may provide additional information about RAO in horses.…”

Section: Introductionmentioning

confidence: 99%

Inhalation of Aspergillus fumigatus spores induces airway inflammation in mice in a similar manner as observed in Recurrent Airway Obstruction in horses

et al. 2011

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In vitro allergy tests compared to intradermal testing in horses with recurrent airway obstruction

Cited by 49 publications

References 34 publications

Development of an <em>in vitro</em> model system for studying the interaction of <em>Equus</em> <em>caballus</em> IgE with its high-affinity receptor FcεRI

Development of an <em>in vitro</em> model system for studying the interaction of <em>Equus</em> <em>caballus</em> IgE with its high-affinity receptor FcεRI

Recurrent airway obstruction in horses - an allergic inflammation: a review

Inhalation of Aspergillus fumigatus spores induces airway inflammation in mice in a similar manner as observed in Recurrent Airway Obstruction in horses

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In vitro allergy tests compared to intradermal testing in horses with recurrent airway obstruction

Cited by 49 publications

References 34 publications

Development of an <em>in vitro</em> model system for studying the interaction of <em>Equus</em> <em>caballus</em> IgE with its high-affinity receptor Fc&#949;RI

Development of an <em>in vitro</em> model system for studying the interaction of <em>Equus</em> <em>caballus</em> IgE with its high-affinity receptor Fc&#949;RI

Recurrent airway obstruction in horses - an allergic inflammation: a review

Inhalation of Aspergillus fumigatus spores induces airway inflammation in mice in a similar manner as observed in Recurrent Airway Obstruction in horses

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Development of an <em>in vitro</em> model system for studying the interaction of <em>Equus</em> <em>caballus</em> IgE with its high-affinity receptor FcεRI

Development of an <em>in vitro</em> model system for studying the interaction of <em>Equus</em> <em>caballus</em> IgE with its high-affinity receptor FcεRI