Development of an &lt;em&gt;in vitro&lt;/em&gt; model system for studying the interaction of &lt;em&gt;Equus&lt;/em&gt; &lt;em&gt;caballus&lt;/em&gt; IgE with its high-affinity receptor Fc&amp;#949;RI

Sabban, Sari; Ye, Hongtu; Helm, Birgit A.

doi:10.3791/52222

Cited by 4 publications

(1 citation statement)

References 29 publications

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“…HLA-A2, having the highest prevalence of MHC class I in the Thai population, was selected for this study. The MNP, Fe 3 O 4 , is considered due to its ability to direct a magnetic field, low toxicity, strong biocompatibility, and high relaxivity [16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34] in a biosensing system based on micro-NMR. Owing to the instability of iron in a strongly acidic environment, MNPs may shorten the lifespan of a material.…”

Section: Introductionmentioning

confidence: 99%

Immobilization of Thai Population-specific Human Leukocyte Antigens on Magnetic Nanoparticles Integrated with Nuclear Magnetic Resonance Technology

Subtaweewasin,

Pijitrojana

2023

Nano Biomedicine and Engineering

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Kidney disease, as a global health problem, can progress to kidney failure. Kidney transplantation is a treatment option for end-stage disease and an alternative to dialysis. Complement-dependent cytotoxicity tests and flow cytometry are methods used to test for compatibility between autoantibodies and donor recipient. Antibodies against donor human leukocyte antigens (HLAs), linked to hyperacute or severely acute rejection, frequently result in graft loss. Currently, fluorescent bead assay (Luminex ® ) technology is used in Thailand for HLA-antibody typing tests. However, each test requires specific location and time, expensive equipment, and high costs. The goal of such research is that, HLA-antibody typing tests can be performed wherever it is the most convenient for recipients and hospitals. A further advantage is that the surgeon can perform a final test for confirmation before the surgery. Numerous technologies are currently available for this purpose. The focus of this study was on the Fe 3 O 4 magnetic nanoparticles (MNPs) immobilized with HLAs frequently found in the Thai population. These were examined using nuclear magnetic resonance (NMR) to measure HLA antibody typing. MNPs have recently attracted considerable attention because of their strong magnetization and large surface areas. Immobilization reactions with antigens and the straightforward magnetic separation of MNPs are low-cost techniques. Hence, the commercialization of Fe 3 O 4 MNPs is favorable. In this study, MNPs were functionalized with 3aminopropyl triethoxysilane (APTES) in toluene and ethanol, and the nanoparticles were streptavidin activated after APTES treatment. A specific biotinylated HLA for the Thai population was used and immobilized on streptavidin. The MNPs were effectively immobilized. The magnetic properties can be modified by adding other ions to Fe 3 O 4 . We discovered that the shape and size variations of Fe 3 O 4 MNPs may have an impact on the amount of functionalized and immobilized surface area.

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Section: Introductionmentioning

confidence: 99%

Immobilization of Thai Population-specific Human Leukocyte Antigens on Magnetic Nanoparticles Integrated with Nuclear Magnetic Resonance Technology

Subtaweewasin,

Pijitrojana

2023

Nano Biomedicine and Engineering

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The influence of amino acid properties on the adsorption of proteins and peptides to stainless steel surfaces.

Chandrasekaran¹

2014

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Evaluation of Optical Detection Platforms for Multiplexed Detection of Proteins and the Need for Point-of-Care Biosensors for Clinical Use

Spindel

Sapsford

2014

Sensors

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This review investigates optical sensor platforms for protein multiplexing, the ability to analyze multiple analytes simultaneously. Multiplexing is becoming increasingly important for clinical needs because disease and therapeutic response often involve the interplay between a variety of complex biological networks encompassing multiple, rather than single, proteins. Multiplexing is generally achieved through one of two routes, either through spatial separation on a surface (different wells or spots) or with the use of unique identifiers/labels (such as spectral separation—different colored dyes, or unique beads—size or color). The strengths and weaknesses of conventional platforms such as immunoassays and new platforms involving protein arrays and lab-on-a-chip technology, including commercially-available devices, are discussed. Three major public health concerns are identified whereby detecting medically-relevant markers using Point-of-Care (POC) multiplex assays could potentially allow for a more efficient diagnosis and treatment of diseases.

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Development of an <em>in vitro</em> model system for studying the interaction of <em>Equus</em> <em>caballus</em> IgE with its high-affinity receptor FcεRI

Cited by 4 publications

References 29 publications

Immobilization of Thai Population-specific Human Leukocyte Antigens on Magnetic Nanoparticles Integrated with Nuclear Magnetic Resonance Technology

Immobilization of Thai Population-specific Human Leukocyte Antigens on Magnetic Nanoparticles Integrated with Nuclear Magnetic Resonance Technology

The influence of amino acid properties on the adsorption of proteins and peptides to stainless steel surfaces.

Evaluation of Optical Detection Platforms for Multiplexed Detection of Proteins and the Need for Point-of-Care Biosensors for Clinical Use

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