In vitro aging

Miller, Robert C.; Nichols, Warren W.; Pottash, J.; Aronson, M.M.

doi:10.1016/0014-4827(77)90270-1

Cited by 41 publications

(6 citation statements)

References 45 publications

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“…The proliferative lifeman diploid fibroblast cell tained under similar condi laboratory resembled those previously . Figure l B fects of serial subcultivatio rate and plateau density of I As the CPDL of the culture small but progressive decre; tial growth rate and a signil the plateau density were n results were obtained for ( strain cloned from a differe Karyotypic analyses of BFA-lc cul--sulted in the tures with Giemsa-banding techniques proliferating gave results that were consistent with -spans of hu-those of other cell systems that exhibit I lines mainsenescence in vitro (12). A normal boitions in this vine male karyotype (modal chromoe determined some number, 60) (13) with a low level of shows the ef-polyploidy (7 percent) was found in cul-)n on growth tures at CPDL 31; a similar level of poly-BFA-lc cells.…”

supporting

confidence: 54%

“…Similar percent of the BFA-lc cells examined, cultures of a and this value increased to 20 percent by -nt fetal calf; CPDL 66. As in other models of cellular senescence in vitro, this increase in chromosomal aberrations accompanied the loss of cellular proliferative capacity (12). 14' In human diploid fibroblasts, such as 0 WI-38 cells, the progressive decline in 0 cell proliferation as the cultures senesce 6o 400 / is a reflection of a progressive decrease !ted M in the fraction of rapidly dividing cells (14,15).…”

mentioning

confidence: 81%

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Cellular Senescence in a Cloned Strain of Bovine Fetal Aortic Endothelial Cells

Mueller

Rosen

Levine

1980

Science

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The life-span in vitro and other proliferative characteristics of a strain of endothelial cells cloned from the aorta of a fetal calf were examined. Cultures of these cells had a replicative life-span of approximately 80 cumulative population doublings. Growth rates in the logarithmic phase and plateau densities decreased as the cumulative population-doubling level increased. After approximately 65 percent of the life-span of a culture was completed, the percentage of cells that incorporated [3H]thymidine during a 24-hour labeling period began to decrease rapidly. The cells expressed factor VIII antigen and their intercellular borders were stainable with silver nitrate throughout the life-span of each culture. Average cellular attachment size increased more than threefold between cumulative population-doubling levels 41 and 80. The facility with which cloned strains of endothelial cells can be isolated should encourage further exploitation of this important cell culture model.

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supporting

confidence: 54%

mentioning

confidence: 81%

Cellular Senescence in a Cloned Strain of Bovine Fetal Aortic Endothelial Cells

Mueller

Rosen

Levine

1980

Science

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“…3 Unfortunately, direct comparisons of the replicative potentials of normal cells in culture obtained in different studies are hampered by the fact that they may vary considerably due to differences in tissues of origin (11,28) and cell types (29) as well as to the specific culture conditions employed, including the nutrient medium, serum, and cultivation procedures (30)(31)(32)(33)(34). Also, intraspecific genetic differences may play a role as suggested from the fact that fibroblast cultures from humans who age prematurely, Werner syndrome and progeria, may undergo fewer PDs if compared to normal cells (8,35,36).…”

Section: Resultsmentioning

confidence: 99%

Evidence for a relationship between longevity of mammalian species and life spans of normal fibroblasts in vitro and erythrocytes in vivo

Röhme

1981

Proc. Natl. Acad. Sci. U.S.A.

310

135

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The replicative life spans of mammalian fibroblasts in vitro were studied in a number of cell cultures representing eight species. Emphasis was placed on determining the population doubling level at which phase III (a period of decrease in the rate of proliferation) and chromosomal alterations occur. All the cell cultures studied went through a growth crisis, a period of apparent growth cessation lasting for at least 2 weeks. In most cultures, the crisis represented the end of their replicative capacities, but in some cultures cell proliferation was resumed after the crisis. A predominantly diploid chromosome constitution (more than 75%) was demonstrated prior to the growth crisis. In cultures in which cell proliferation was resumed after the crisis, a nondiploid constitution prevailed in all cases except the rat (with 90% or more diploid cells all the time). The growth crisis occurred at population doubling levels that were characteristic for the species and was shown to be related to the species' maximal life span by a strict power law, being proportional to the square root of the maximal life span. Based on data in the literature, the same relationship was also valid for the lifespans ofcirculating mammalian erythrocytes in vivo. These results may indicate the prevalence of a common functional basis regulating the life span of fibroblasts and erythrocytes and thus operating in replicative as well as postmitotic cells in vitro and in viva

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“…Other studies have shown a similar low incidence of cytogenetic abnormalities, even in long-term cultures of human fibroblasts which were mostly of normal fetal or infant origin, and it was only in senescent cells that an increased occurrence of chromosomal aberra tions, aneuploidy, and polyploidy was found; loss of sex chromosomes or evidence of clonal growth was not noted (Hayflick and Moorhead, 1961;Saksela and Moorhead, 1963;Hayflick, 1965;Thompson and Holliday, 1975;Benn. 1976;Miller et al, 1977). How ever, cytogenetic clones have been found in skin fibro blasts of adult origin.…”

Section: Discussionmentioning

confidence: 99%

Sex chromosome loss and clones characterized by autosomal abnormality in cultured corneal fibroblasts of man

Fitzgerald¹,

Rosman²

1984

Cytogenet Genome Res

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Cultured fibroblasts from 7 of 14 corneas showed loss of a sex chromosome in 20–100% of metaphase cells. An X chromosome was lost from female cells, a Y from male cells. Sex chromosome loss was more common in ophthalmologically abnormal corneas but did not show a strong correlation with donor age or with longer time in culture. Clones characterized by autosomal aneuploidy and aberrations were present in corneal cultures from seven donors. Their presence was not related to the incidence of sex chromosomal loss or prolonged culture time. Our results suggest that human corneal cells have a tendency toward loss of a sex chromosome and toward autosomal change, and that such cells form clones. It has yet to be determined if corneal cells undergo these cytogenetic changes and clone formation in vivo.

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In vitro aging

Cited by 41 publications

References 45 publications

Cellular Senescence in a Cloned Strain of Bovine Fetal Aortic Endothelial Cells

Cellular Senescence in a Cloned Strain of Bovine Fetal Aortic Endothelial Cells

Evidence for a relationship between longevity of mammalian species and life spans of normal fibroblasts in vitro and erythrocytes in vivo

Sex chromosome loss and clones characterized by autosomal abnormality in cultured corneal fibroblasts of man

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