In vitro activity of the interaction between taxifolin (dihydroquercetin) and pyrimethamine against <i>Toxoplasma gondii</i>

Abugri, Daniel A.; Witola, William H.; Russell, Albert E.; Troy, Roberta M.

doi:10.1111/cbdd.13070

Cited by 24 publications

(8 citation statements)

References 57 publications

(80 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous reports showed possible beneficial effects of taxifolin using animal models, including improvement of microcirculation [5], hepatoprotective effects [6], anti-viral activity [7], and prevention of diabetic nephropathy [8] as well as cardiomyopathy [9]. Further, in vitro studies demonstrated that taxifolin exhibits anti-bacterial [10], anti-fungal [11], and anti-parasitic [12] effects, and taxifolin inhibits acetylcholinesterase and carbonic anhydrase isoenzymes [13]. Taxifolin also inhibits oligomer formation of amyloid β proteins in mice, and is thought to be effective to prevent Alzheimer related diseases [14].…”

Section: Introductionmentioning

confidence: 99%

Cell cytotoxity and anti-glycation activity of taxifolin-rich extract from Japanese larch, Larix kaempferi

Muramatsu

Uchiyama

Kida

et al. 2019

Heliyon

View full text Add to dashboard Cite

The larches, the Larix genus of plants are known as a natural source of taxifolin (dihydroquercetin), and extracts of its taxifolin rich xylem are used in dietary supplements to maintain health. In the present study, to assess biological activities of a methanol extract of the Japanese larch, Larix kaempferi (LK-ME), the effects of LK-ME on cell viability, inflammatory cytokine expression, and glycation were investigated. The effects of taxifolin which is known to be a main compound of LK-ME, and its related flavonoids, quercetin and luteolin were also examined. The results show that taxifolin exhibits lower growth inhibition activity and lesser induction activity of inflammatory cytokines in a human monocyte derived cell line, THP-1 cells, while in vitro anti-glycation activities of taxifolin were inhibiting at comparable levels to those of quercetin and luteolin. The growth inhibition and the cytokine induction activities, and the anti-glycation effects of LK-ME are assumed to have properties similar to taxifolin. The results of high performance liquid chromatography (HPLC) analysis indicated that taxifolin was detected as the main peak of LK-ME at the absorbance of 280 nm, and the concentration of taxifolin was measured as 3.12 mg/ml. The actual concentration of taxifolin in LK-ME is lower than the concentration estimated from the IC 50 values calculated by the results of glycation assays, suggesting that other compounds contained in LK-ME are involved in the anti-glycation activity.

show abstract

Section: Introductionmentioning

confidence: 99%

Cell cytotoxity and anti-glycation activity of taxifolin-rich extract from Japanese larch, Larix kaempferi

Muramatsu

Uchiyama

Kida

et al. 2019

Heliyon

View full text Add to dashboard Cite

show abstract

“…Taxifolin is known to be an antioxidant agent [3], and is known for the many beneficial effects of taxifolin, such as improvement of microcirculation [4], hepatoprotective effects [5], anti-viral activity [6], and prevention of diabetic nephropathy [7] as well as diabetic cardiomyopathy [8]. In vitro studies have demonstrated that taxifolin exhibits anti-bacterial [9], anti-fungal [10], and anti-parasitic [11] effects, and also inhibitory activities against acetylcholinesterase and carbonic anhydrase isoenzymes [12]. Further, oligomer formation of amyloid β proteins is significantly inhibited by taxifolin in mice, suggesting that taxifolin is effective to prevent Alzheimer's disease [13].…”

Section: Introductionmentioning

confidence: 99%

In vitro anti-inflammatory and anti-lipid accumulation properties of taxifolin-rich extract from the Japanese larch, Larix kaempferi

Muramatsu

Uchiyama

Kida

et al. 2020

Heliyon

View full text Add to dashboard Cite

The Japanese larch, ( Larix kaempferi ) is known to contain abundant taxifolin (dihydroquercetin) in its xylem. In this study, to assess the bioactivities of taxifolin rich methanol extract of L. kaempferi (LK-ME), anti-inflammatory effect, and the anti-lipid accumulation effect of LK-ME were investigated. The results showed that nitric oxide (NO) and reactive oxygen species (ROS) were reduced after treatment with LK-ME, and that lipid accumulation in adipocyte differentiated 3T3-L1 cells was inhibited after the cells were grown in medium containing LK-ME. Taxifolin, the major compound contained in LK-ME, and its related compounds, quercetin and luteolin also exhibited similar effects with LK-ME. The LK-ME exhibits relatively strong anti-inflammatory and anti-lipid accumulation activities compared with that of similar amounts of taxifolin contained in LK-ME, suggesting that other minor compounds contained in LK-ME is involved in the effects. These results indicate the potential of taxifolin-rich L. kaempferi extract for use as a supplement to prevent excess inflammation and obesity.

show abstract

“…The safety of DHQ also has been investigated since it is a key component of dietary supplements 2 . Additionally, DHQ has a wide range of pharmacological activities, including anti-inflammatory 3 , anti-microbial 4 anti-cancer 5 , anti-Alzheimer 6 , anti-toxoplasmosis effects 7 , health-promoting effects on hepatoprotective and cardiovascular systems 8 , 9 . Due to its excellent pharmacological activity, DHQ is routinely used in pharmaceuticals, health products, foods and agriculture.…”

Section: Introductionmentioning

confidence: 99%

Exploring the genes involved in biosynthesis of dihydroquercetin and dihydromyricetin in Ampelopsis grossedentata

Zhang

Jiang

et al. 2021

Sci Rep

View full text Add to dashboard Cite

Dihydroquercetin (DHQ), an extremely low content compound (less than 3%) in plants, is an important component of dietary supplements and used as functional food for its antioxidant activity. Moreover, as downstream metabolites of DHQ, an extremely high content of dihydromyricetin (DHM) is up to 38.5% in Ampelopsis grossedentata. However, the mechanisms involved in the biosynthesis and regulation from DHQ to DHM in A. grossedentata remain unclear. In this study, a comparative transcriptome analysis of A. grossedentata containing extreme amounts of DHM was performed on the Illumina HiSeq 2000 sequencing platform. A total of 167,415,597 high-quality clean reads were obtained and assembled into 100,584 unigenes having an N50 value of 1489. Among these contigs, 57,016 (56.68%) were successfully annotated in seven public protein databases. From the differentially expressed gene (DEG) analysis, 926 DEGs were identified between the B group (low DHM: 210.31 mg/g) and D group (high DHM: 359.12 mg/g) libraries, including 446 up-regulated genes and 480 down-regulated genes (B vs. D). Flavonoids (DHQ, DHM)-related DEGs of ten structural enzyme genes, three myeloblastosis transcription factors (MYB TFs), one basic helix–loop–helix (bHLH) TF, and one WD40 domain-containing protein were obtained. The enzyme genes comprised three PALs, two CLs, two CHSs, one F3’H, one F3’5’H (directly converts DHQ to DHM), and one ANS. The expression profiles of randomly selected genes were consistent with the RNA-seq results. Our findings thus provide comprehensive gene expression resources for revealing the molecular mechanism from DHQ to DHM in A. grossedentata. Importantly, this work will spur further genetic studies about A. grossedentata and may eventually lead to genetic improvements of the DHQ content in this plant.

show abstract

In vitro activity of the interaction between taxifolin (dihydroquercetin) and pyrimethamine against Toxoplasma gondii

Cited by 24 publications

References 57 publications

Cell cytotoxity and anti-glycation activity of taxifolin-rich extract from Japanese larch, Larix kaempferi

Cell cytotoxity and anti-glycation activity of taxifolin-rich extract from Japanese larch, Larix kaempferi

In vitro anti-inflammatory and anti-lipid accumulation properties of taxifolin-rich extract from the Japanese larch, Larix kaempferi

Exploring the genes involved in biosynthesis of dihydroquercetin and dihydromyricetin in Ampelopsis grossedentata

Contact Info

Product

Resources

About