In-vitro activity of taurolidine on single species and a multispecies population associated with periodontitis

Zollinger, Lilly; Schnyder, Simone; Nietzsche, Sándor; Sculean, Anton; Eick, Sigrun

doi:10.1016/j.anaerobe.2014.11.008

Cited by 16 publications

(19 citation statements)

References 29 publications

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“…A clear concentration-dependent activity for taurolidine was confirmed. But, in contrast to our previous results on a 2 % solution [12], the mixed population was not eliminated by the gel formulations. Elimination of multi-species mixtures per se might be critical.…”

Section: Discussioncontrasting

confidence: 99%

“…First, the surface might be the reservoir of the antimicrobial; later, an equal distribution in the medium (concentration about 0.04 %) can be assumed. In comparison, taurolidine in solution with a final concentration of 0.1 % was more active in inhibiting biofilm formation [12]. This underlines again the necessity of a high concentration of taurolidine over a longer time period.…”

Section: Discussionmentioning

confidence: 79%

“…Similar effects have been described before [10] and are of importance since gingival crevicular fluid contains up to 35 % of the albumin found in serum [11]. In a subsequent study, we compared taurolidine with minocycline against selected species associated with periodontitis and against a multi-species mixture consisting of 12 species [12]. The working concentration was chosen up to 50 % of the minimal bactericidal concentration (MBC) against the mixed population in planktonic stage.…”

Section: Introductionmentioning

confidence: 92%

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In vitro activity of taurolidine gel on bacteria associated with periodontitis

et al. 2015

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 92%

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In vitro activity of taurolidine gel on bacteria associated with periodontitis

et al. 2015

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“…Therefore, besides addressing a clinical efficacy of a mouthwash, it is important to investigate its effects on the oral microbiome [9]. For this purpose, in vitro biofilm models offer an approach to elucidate the microbiological and ecological effect of potential products at an early stage of product development [10–15]. …”

Section: Introductionmentioning

confidence: 99%

Effect of mouthwashes on the composition and metabolic activity of oral biofilms grown in vitro

et al. 2016

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ObjectiveThe aim of this study was to determine the effect of an oxygenating mouthwash compared to two other established mouthwash products on bacterial composition and metabolic activity of oral biofilms in vitro.Material and methodsTwelve healthy subjects participated as donors. Plaque-saliva mixture inoculated biofilms were grown and treated with 3 different chemotherapeutic mouthwashes [amine fluoride/stannous fluoride (MD), oxygenating agent (AX), chlorhexidine 0.12 % (PA), and water (W)]. Effects of treatments were assessed on biofilm composition (16S rRNA gene amplicon sequencing), production of organic acids (formate, acetate, lactate, propionate, butyrate using capillary electrophoresis), and viability of the remaining biofilm (CFUs).ResultsMicrobial profiles of biofilms clustered per inoculum donor and were dominated by the genera Veillonella, Streptococcus, and Prevotella. Microbial diversity was only reduced after PA treatment. Significant changes in composition occurred after treatment with AX, resulting in lower proportions of Veillonella and higher proportions of non-mutans streptococci. Production of all organic acids after PA and lactate after MD was significantly lower as compared to W. AX resulted in reduction of acetate, butyrate, and propionate and increase in lactate production (p < 0.05). Viable counts were significantly lower after PA and AX treatments compared to W, while no significant reduction was observed after MD.ConclusionsAll studied mouthwashes affected the in vitro biofilms differently. The effects of the AX treatment were the most prominent which resulted in changes of the bacterial composition and metabolism.Clinical implicationsAwareness by the dental team that mouthwashes can change the bacterial composition and metabolism is important when advising its use.

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“…Zollinger et al demonstrated that antibacterial effects of taurolidine were substantially reduced in a 12-species multispecies biofilm compared with single-species biofilm. 63 Previous study showed that F. nucleatum coaggregated with P. gingivalis , and both species coaggregated with S. gordonii , playing a synergistic effect. 9 Therefore, two bacterial mixtures of P. gingivalis and F. nucleatum resulted in greater survival than the monocultures.…”

Section: Discussionmentioning

confidence: 97%

Novel multifunctional dental bonding agent for class-V restorations to inhibit periodontal biofilms

Wang

Weir

et al. 2017

RSC Adv.

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We recently developed a dental bonding agent to bond restorations to teeth using nanoparticles of amorphous calcium phosphate (NACP) for remineralization with rechargeable calcium and phosphate ion release. The objectives of this study were to: (1) incorporate an antibacterial monomer dimethylaminohexadecyl methacrylate (DMAHDM) and a protein-repellent agent 2-methacryloyloxyethyl phosphorylcholine (MPC); and (2) investigate protein adsorption and periodontitis-related biofilms for the first time. A primer, used to prime tooth structures for bonding, was made with pyromellitic glycerol dimethacrylate (PMGDM) and 2-hydroxyethyl methacrylate (HEMA). An adhesive was made with PMGDM, ethoxylated bisphenol A dimethacrylate and HEMA. NACP, MPC and DMAHDM were incorporated. Streptococcus gordonii, Actinomyces naeslundii, Porphyromonas gingivalis, Fusobacterium nucleatum were cultured to form single and multi-species biofilms. Colony-forming units (CFU), live/dead, metabolic activity, and polysaccharide were measured. Adding DMAHDM, MPC and NACP into the bonding agent did not compromise the dentin bond strength (p > 0.1). Bonding agents with 5% MPC reduced protein adsorption to 1/15 that of the control (p < 0.05). Bonding agents with 5% DMAHDM + 5% MPC had much greater reduction in biofilms than DMAHDM or MPC alone (p < 0.05). Biofilm CFU was reduced by 3 to 4 log via DMAHDM + MPC. Metabolic activities and polysaccharide of biofilms were also substantially reduced (p < 0.05). In conclusion, a novel bonding agent was developed for dental restorations with inhibition of biofilms, reducing CFU by 3 to 4 log. Besides remineralizartion and acid-neutralization via NACP to inhibit caries as shown previously, the multifunctional adhesive is promising for root restorations with subgingival margins to suppress periodontal pathogens and protect the periodontium.

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In-vitro activity of taurolidine on single species and a multispecies population associated with periodontitis

Cited by 16 publications

References 29 publications

In vitro activity of taurolidine gel on bacteria associated with periodontitis

In vitro activity of taurolidine gel on bacteria associated with periodontitis

Effect of mouthwashes on the composition and metabolic activity of oral biofilms grown in vitro

Novel multifunctional dental bonding agent for class-V restorations to inhibit periodontal biofilms

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