In vitro Activity of Six Antifungal Agents on Clinical Dermatophytes

Pakshir, Keyvan; Bahaedinie, L.; Rezaei, Zahra; Sodaifi, Manouchehr; Zomorodian, Kamiar

doi:10.1016/j.ijid.2008.05.762

Cited by 35 publications

(47 citation statements)

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“…They comprise a phylogenetically closely related group of genera with numerous species. They attack the keratinized tissues and cause a wide spectrum of clinical manifestations that vary from mild to severe [6].…”

Section: Discussionmentioning

confidence: 99%

“…Development and standardization of antifungal susceptibility test have shown remarkable progress in the field of medical mycology [6], although, studies using the E-test method for dermatophytes susceptibilities is not yet sufficient. In a limited number of studies, showed that E-test seems to be an alternative method to MIC-determination of antifungal drugs for dermatophytes, since it is a lesslaborious methodology and results could be obtained faster [4,9,21,22].…”

Section: Discussionmentioning

confidence: 99%

“…Additional tests included those for the ability to produce a red pigment when the strains were grown on Potato Dextrose Agar (PDA) and for the ability to produce urease, as well as the hair perforation test. Strains were stored -70 0 C until the time of use, and prior to testing were sub-cultured on PDA at 28 0 C for 15 days to ensure optimal growth characteristics [1,3,6]. All procedures in the experimental protocol were approved by The Ethics Committee of Medical Faculty.…”

Section: Methodsmentioning

confidence: 99%

“…The organisms belong to three genera, Trichophyton, Epidermophyton, and Microsporum. Infections caused by these fungi are among the most prevalent cutaneous infections globally and the recent increase in the number of patients with immunocompromised states, such as AIDS, diabetes mellitus, cancer and organ transplantation has given these infections more prominence [1][2][3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

“…However, these methods are time-consuming and labour-intensive, and are not practical for the clinical laboratory. Therefore, simple alternative approaches are needed [1,3,4,[6][7][8].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Investigation of In Vitro Activity of Five Antifungal Drugs against Dermatophytes Species Isolated from Clinical Samples Using the E-Test Method

Aktaş

Yiğit

Aktaş

et al. 2014

EAJM

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Objective: Dermatomycosis is an infection with fungi related to the skin: glabrous skin, hair and/or nails. Oral treatment of fungal infections in dermatology has become a preferred modality for the management of these very common conditions. Although there are increasing numbers of antifungals available for treatment of dermatophytes, some cases and relapses have been unresponsive to treatment. The determination of fungus in-vitro antifungal susceptibility has been reported to be important for the ability to eradicate dermatophytes. It is necessary to perform antifungal susceptibility testing of dermatophytes. E-test (AB Biodisk, Sweden) is a rapid, easy-toperform in-vitro antifungal susceptibility test. The aim of this study was to investigate the susceptibility of the different species of dermatophyte strains isolated clinical specimens to five antifungal agents using the E-test method. Materials and Methods:A total of 66 specimens were collected from the nails, feet, inguinal region, trunk and hands. These strains tested MIC endpoints of E-test for amphotericin B, fluconazole, itraconazole, caspofungin, and ketoconazole were read after 72, and 96 hours incubation for each strain on RPMI 1640 agar. Results:The dermatophytes tested included Trichophyton rubrum 43 (65.1%), Trichophyton mentagrophytes 7 (10.7%), Microsporum canis 5 (7.6%), Trichophyton tonsurans 5 (7.6%), Epidermophyton floccosum 4 (6.0%) and Trichophyton violaceum 2 (3.0%). The most active agent against all dermatophytes species was caspofungin with a minimal inhibitory concentration (MIC) range (μg/mL ) (0, 19-48, 2-256, 2-8, 256, 256, 8-24).Conclusion: E-test seems to be an alternative method to MIC-determination of antifungal drugs for dermatophytes species, since it is a less-laborious methodology and results could be obtained faster. Key Words: Dermatophytes, E-test, antifungal ÖzetAmaç: Dermatomikozlar mantarlar tarafından oluşturulan deri, saç ve tırnakları tutan enfeksiyonlardır. Dermatolojide mantar enfeksiyonlarının oral tedavisi yaygın olarak tercih edilen yöntem haline gelmiştir. Dermatofitlerin tedavisi için kullanılan antifungallerin sayısının artmasına rağmen bazı durumlarda tedaviye yanıtsızlık ve nüksler gelişmektedir. İn-vitro antifungal duyarlılığın belirlenmesinin dermatofitlerin etkili olarak tedavi edilmesi için önemli olduğu bildirilmektedir. Antifungal duyarlılığın belirlenmesinde yöntemler önemlidir. E-test (AB Biodisk, İsveç) hızlı ve kolay uygulanabilir bir duyarlılık yöntemidir. Bu çalışmanın amacı, klinik örneklerden izole edilen farklı dermatofit türlerinin beş antifungal ilaca karşı duyarlılık-larını E-test yöntemi ile belirlemektir. ) aralıkları ile itrakonazol olarak bulundu. En az etkili antifungal ise (0, 19-48, 2-256, 2-8, 256, 256, 8-24) MIC (μg/mL -1 ) aralıkları ile flukonazol olarak bulundu. Gereç veSonuç: E-test dermatofit türlerinin antifungal duyarlılığının belirlenmesinde daha az zahmetli ve sonuçların daha hızlı elde edilebilir olması ile alternatif bir yöntem gibi görünmektedir.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…However, these methods are time-consuming and labour-intensive, and are not practical for the clinical laboratory. Therefore, simple alternative approaches are needed [1,3,4,[6][7][8].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Investigation of In Vitro Activity of Five Antifungal Drugs against Dermatophytes Species Isolated from Clinical Samples Using the E-Test Method

Aktaş

Yiğit

Aktaş

et al. 2014

EAJM

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Solid and liquid green Ag nanoparticles based on banana peel extract as an eco‐friendly remedy for ringworm in pets

Emam

Eisa

Hasanin

2022

Surface & Interface Analysis

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Green chemistry‐based biowaste has been taken attention toward new components for many applications especially in dermatophytes of animals and humans. The main goal of this study is to assist the synthetic pathways; clean and large‐scale synthesis of silver nanoparticles is achieved as a promising alternative green technique with high efficiency, simplicity and less hazardous waste based on agro‐waste of banana peels extract powder. The preparation of silver nanoparticles is directly via solid‐state reaction and the liquid one based on the natural agro‐waste of banana peels under specific conditions for treating ringworm in pets. Ultraviolet (UV)‐visible spectroscopy, Fourier transform infrared spectroscopy (FT‐IR), and transmission electron microscope (TEM) were used to prove the prepared nanoparticles. A total of 30 samples from pets (dogs and cats) were subjected to mycological examination. Ten (33.3%) were culture positive, and 100% of the positive samples were Microsporum canis. In vitro antifungal activity against M. canis isolates using both the disc diffusion and the broth microdilution methods was evaluated. The significant results for solid silver nanoparticles (Ags1 and Ags2) than liquid Ag NPs (AgL) with p < 0.05 compared with itraconazole were observed. This biomaterial from waste could be used as a fast, simple, eco‐friendly, and efficient method to prepare Ag NPs on solid that can be applied in the veterinary sector against ringworm.

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Antifungal Activities of Curcuminoids and Difluorinated Curcumin Against Clinical Dermatophyte Isolates

Zarrinfar

Behnam

Hatamipour

et al. 2021

Advances in Experimental Medicine and Biology

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In vitro Activity of Six Antifungal Agents on Clinical Dermatophytes

Cited by 35 publications

References 0 publications

Investigation of In Vitro Activity of Five Antifungal Drugs against Dermatophytes Species Isolated from Clinical Samples Using the E-Test Method

Investigation of In Vitro Activity of Five Antifungal Drugs against Dermatophytes Species Isolated from Clinical Samples Using the E-Test Method

Solid and liquid green Ag nanoparticles based on banana peel extract as an eco‐friendly remedy for ringworm in pets

Antifungal Activities of Curcuminoids and Difluorinated Curcumin Against Clinical Dermatophyte Isolates

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