In vitro activity of propolis: Synergism in combination with antibiotics against Staphylococcus spp.

Silva, Wellington Erasmo de Lima; Ferrari, Wilson Duarte; Rosa, Patricia Ramos da; Peixoto, Rodolfo de Moraes; Tenório, Jonh Aldson Bezerra; Silva, Tânia Maria Sarmento da; Costa, Mateus Matiuzzi da

doi:10.5897/ajmr2014.7173

Cited by 3 publications

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References 19 publications

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“…In both extracts the total phenol content was higher than the total flavonoids content, a common finding for propolis from Brazil (Alencar et al 2007, Kalogeropoulos et al 2009, Nunes et al 2012, Coelho 2013, Greece, Cyprus (Kalogeropoulos et al 2009), Czech Republic, Ireland and Germany (Al-Ani et al 2018). The total phenol and flavonoids contents observed for EPA were similar to those of Kumazawa et al (2004), El Sohaimy & Masry (2014) and Silva et al (2015), lower than Alencar et al (2007), Cabral et al (2012), Peixoto et al (2012) and El Sohaimy & Masry (2014), and higher than Tiveron et al (2016). Cunha et al (2004) observed a variation of 6.41 to 15.24% in the total phenol content, while Gonsales et al (2006) verified variations between 0.05 and 0.63% in the contents of flavonoids and Sousa et al (2007) verified a total flavonoid content of 0.06 to 0.38% for samples from São Paulo (Franca region) and from 0.12 to 2.11% for those from Minas Gerais (Passo region).…”

Section: Discussionsupporting

confidence: 63%

Chemical composition and antimicrobial activity of two extract of propolis against isolates of Staphylococcus spp. and multiresistant bacterials

et al. 2019

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Amarante J.F., Ribeiro M.F., Costa M.M., Menezes F.G., Silva T.M.S., Amarante T.A.B., Gradela A. & Moura L.M.D. 2019. Chemical composition and antimicrobial activity of two extract of propolis against isolates of Staphylococcus spp. and multiresistant bacterials. Pesquisa Veterinária Brasileira 39 (9):734-743. There is a growing need to discover and develop alternative therapies for the treatment of mastitis caused by Staphylococcus spp. and multidrug-resistant bacterial infections. This study examined the chemical composition and antimicrobial potential of two propolis extracts (EPA and EPB) against seventy-seven isolates of Staphylococcus spp. obtained from subclinical bovine mastitis; three clinical strains of MRSA and two from clinical strains of S. aureus ATCC, identified as S. aureus ATCC 6538 and S. aureus ATCC 25923. The total phenolic content was determined by the Folin-Ciocalteau method, the total flavonoid content by the Dowd method and the phenolic profile was quantified by HPLC-DAD. The MBC values of the extracts were evaluated by broth microdilution method. The amount of total phenolic and flavonoid compounds was higher in EPA than EPB. Both extracts revealed the presence of caffeic, coumaric, cinnamic, ferulic and 3,4-dihydroxybenzoic acids, with higher concentrations of coumaric and cinnamic acids. Staphylococcus spp. isolates were susceptible to EPA (90.9%), EPB (83.1%) and oxacillin (80.5%). The oxacillin susceptible isolates were also susceptible to EPA (70.1%) and EPB (80.6%), whereas those oxacillin-resistant strains were also susceptible to EPA (40.0%) and to EPB (26.7%). MBC ranged from 34.3 to 68.7μm/mL for EPA and from 68.7 to 137.5μg/mL for EPB. Both extracts inhibited significantly (100%) the clinical strains of MRSA, S. aureus ATCC 6538 and S. aureus ATCC 25923 at the concentration of 68.7μg/mL. It is concluded that both extracts of propolis, whose main constituents are coumaric and cinnamic acids, have high antimicrobial activity against the microorganisms studied, and EPA also against oxacillin-resistant strains. These findings reinforce its potential use for the treatment of bovine mastitis.

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Section: Discussionsupporting

confidence: 63%

Chemical composition and antimicrobial activity of two extract of propolis against isolates of Staphylococcus spp. and multiresistant bacterials

et al. 2019

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Antimicrobial activity of propolis and gentamycin against methicillin-resistant Staphylococcus aureus in a 3D thermo-sensitive hydrogel

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Ulucan

et al. 2019

Industrial Crops and Products

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Propolis antibacterial and antioxidant synergisms with gentamicin and honey

Freitas

Cunha

Oliveira

et al. 2022

Journal of Applied Microbiology

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Aims: Assess the antibacterial and antioxidant effects of ethanol extracts of Portuguese propolis samples when combined with gentamicin, a widely used aminoglycoside antibiotic, or with honey, collected from the same apiary as propolis. Methods and Results:Using the agar dilution method and DPPH free radical scavenging assay, antimicrobial and antioxidant synergistic effects between propolis and gentamicin or honey were assessed. Synergism between propolis and gentamicin was observed for all the tested extracts and against all the indicator bacteria, with particular interest against the Methicillin-Resistant Staphylococcus aureus (MRSA) with a threefold decrease of the gentamicin MIC if mixed with 25 µg ml −1 propolis.Likely to propolis and gentamicin, mixtures of sub-MIC concentrations of propolis and honey enhanced the antibacterial action of each individual natural product against the majority of the strains. However, propolis antioxidant capacity decreased along with higher honey content in the mixture.Conclusions: Propolis has a strong synergistic effect when combined with gentamicin, allowing the reduction of the therapeutic dose of this drug. Propolis and honey mixtures also display a stronger antibacterial effect than the activity exhibited by each sample when tested individually, whereas the high antioxidant capacity of propolis seems to be not affected when in combination with honey. Significance and Impact of Study: When mixed with honey, propolis antioxidant potential is maintained, or just slightly reduced up to 1:1 dilution, and show synergistic antibacterial effects, allowing to optimize the use of this usually scarce natural resource. Also, considering the antibiotic resistance problem, natural beehive products, alone or in the mixture, are promising alternatives to retard the outbreak of microbial resistance.

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In vitro activity of propolis: Synergism in combination with antibiotics against Staphylococcus spp.

Cited by 3 publications

References 19 publications

Chemical composition and antimicrobial activity of two extract of propolis against isolates of Staphylococcus spp. and multiresistant bacterials

Chemical composition and antimicrobial activity of two extract of propolis against isolates of Staphylococcus spp. and multiresistant bacterials

Antimicrobial activity of propolis and gentamycin against methicillin-resistant Staphylococcus aureus in a 3D thermo-sensitive hydrogel

Propolis antibacterial and antioxidant synergisms with gentamicin and honey

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