Antimicrobial activity of propolis and gentamycin against methicillin-resistant Staphylococcus aureus in a 3D thermo-sensitive hydrogel

Gezgin, Yüksel; Kazan, Aslihan; Ulucan, Fulden; Yeşil-Çeliktaş, Özlem

doi:10.1016/j.indcrop.2019.111588

Cited by 14 publications

(13 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…During the monitoring of the viscosity, the temperature difference of the solution was also followed by a digital thermometer, as the sudden increase of viscosity pointed to the gelation temperature of the related solution. The gelation temperatures of hydrogels were also confirmed using the magnetic stirrer method 26 . Briefly, POL and HEP3 solutions at the same concentrations (20%, 25%, 30% w/v) were placed into the glass transparent vials with a digital thermometer, then started stirring on the magnetic stirrer.…”

Section: Methodsmentioning

confidence: 99%

“…These porosity results are consistent with another study, where the porosity of 25% (w/w) P407 hydrogel was measured as 60% at 0.02 mm, which was reported to be suitable for cell proliferation. 26 In another study, μ-CT analysis of SAG-based hydrogel produced for bone damage showed that the porosity and pore size of the hydrogel resembled a natural ECM required for implants with pore sizes of about 200-550 μm, found to be favorable for cell mobility and nutrient transport. 31 The thermo-responsive hydrogels showed fluid-like behavior below their specific gelation temperatures, while exhibiting gel-like behavior at temperatures above.…”

Section: Synthesis and Characterization Of Injectable Heparin Conjuga...mentioning

confidence: 97%

“…The gelation temperatures of hydrogels were also confirmed using the magnetic stirrer method. 26 Briefly, POL and HEP3 solutions at the same concentrations (20%, 25%, 30% w/v) were placed into the glass transparent vials with a digital thermometer, then started stirring on the magnetic stirrer. When the magnetic bar stopped stirring, the observed temperature of the solution was recorded as the gelation temperature.…”

Section: Determination Of Rheological Propertiesmentioning

confidence: 99%

See 2 more Smart Citations

Synthesis of an injectable heparin conjugated poloxamer hydrogel with high elastic recoverability for temporomandibular joint disorders

Deliogullari

Ilhan‐Ayisigi

Cakmak

et al. 2022

J of Applied Polymer Sci

Self Cite

View full text Add to dashboard Cite

The temporomandibular joint (TMJ) is commonly affected during fundamental oral activities, reducing the quality of life. Herein, we synthesized a heparinconjugated poloxamer hydrogel (HEP) as a thermo-responsive injectable hydrogel for the treatment of TMJ disorders. While the gelation temperature of synthesized HEP (25% [w/v]) was 29.8-30.0 C, there was a slight difference between loss and storage modulus. HEP decreased the friction of the TMJ, thus requires less energy during load-bearing jaw movement in comparison to POL. Moreover, the oscillation test dependent on strain ranges from 0.01% to 1000% validated that POL and HEP3 hydrogels showed a similar critical strain of about 5.6%. The total elastic recovery percentage of HEP3 (53.50%) was higher than POL (45.55%), indicating a better recovery of the deformed hydrogel structure. Along with the suitable viscoelastic properties for temporomandibular cavity, both hydrogels increased the proliferation of fibroblasts (L929) and chondrocytes (ATDC5) (cell viabilities were above 100%). However, newly synthesized HEP induced differentiated cell proliferation of chondrogenic cells at increasing concentrations up to 0.0156 mg/mL (p < 0.0001) compared to POL and the control group. The promising rheological properties and effects on chondrogenic cell proliferation of injectable heparin-conjugated hydrogel make them candidates for intra-articular injections used for the treatment of TMJ.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Synthesis and Characterization Of Injectable Heparin Conjuga...mentioning

confidence: 97%

Section: Determination Of Rheological Propertiesmentioning

confidence: 99%

See 1 more Smart Citation

Synthesis of an injectable heparin conjugated poloxamer hydrogel with high elastic recoverability for temporomandibular joint disorders

Deliogullari

Ilhan‐Ayisigi

Cakmak

et al. 2022

J of Applied Polymer Sci

Self Cite

View full text Add to dashboard Cite

show abstract

“…Thermosensitive hydrogels were formed through physical cross-linking, which mainly includes electrostatic interaction, hydrogen bond, and hydrophobic force. When these forces acted together and changed, sol-gel phase transitions occurred in different environments (Gezgin et al 2019). Many natural polymers and synthetic polymers could be used to prepare various thermosensitive hydrogels, such as chitosan, collagen, gelatin and so on.…”

Section: Introductionmentioning

confidence: 99%

Design and evaluation of chitosan-amino acid thermosensitive hydrogel

Tong

Zhou

et al. 2021

Mar Life Sci Technol

View full text Add to dashboard Cite

Chitosan/glycerophosphate thermosensitive hydrogel crosslinked physically was a potential drug delivery carrier; however, long gelation time limits its application. Here, chitosan-amino acid (AA) thermosensitive hydrogels were prepared from chitosan (CS), αβ-glycerophosphate (GP), and l-lysine (Lys) or l-glutamic acid (Glu). The prepared CS-Lys/GP and CS-Glu/GP hydrogel showed good thermosensitivity and could form gels in a short time. The optimal parameters of CS-Lys/ GP hydrogel were that the concentration of CS-Lys was 2.5%, the ratio of CS/Lys was 3.5/1.0, the ratio of CS-Lys/GP was 4.5/1.0. The optimal parameters of CS-Glu/GP hydrogel were that the concentration of CS-Glu was 3.0%, the ratio of CS/ Glu was 2.0/1.0, and the ratio of CS-Glu/GP was 4.0/1.5. Chitosan-amino acid (CS-AA) thermosensitive hydrogel had a three-dimensional network structure. The addition of model drug tinidazole (TNZ) had no obvious effect on the structure of hydrogel. The results of infrared spectroscopy showed that there were hydrogen bonds between amino acids and chitosan. In vitro release results showed that CS-Lys/GP and CS-Glu/GP thermosensitive hydrogels had sustained release effects. Thus, the chitosan-amino acid thermosensitive hydrogels hold great potential as a sustained release drug delivery system.

show abstract

“…It is a resinous material collected from different plant parts by honeybees ( Apis mellifera L ) [ 8 ]. Recently propolis has attracted great interest as a potential antimicrobial agent [ 9 ]. Propolis composition is primarily affected by the geographical and climatic changes as well as the botanical origin [ 10 ].…”

mentioning

confidence: 99%

Potential anti-COVID-19 Activity of Egyptian Propolis Using Computational Modeling

et al. 2021

View full text Add to dashboard Cite

Aim: To investigate the prospective anti COVID-19 activity of Egyptian propolis. Material & methods: Propolis samples were collected from different Egyptian geographical areas and characterized using standardized methods, scanning electron microscope and gas chromatography/mass spectrometry along with computational modeling to predict the anti-COVID-19 activity. Results & conclusion: Gas chromatography/mass spectrometry analysis of Menoufia propolis proved the presence of Octatriacontyl pentafluoropropionate (4.2%). Docking analyses declared that Octatriacontyl pentafluoropropionate is well oriented inside the enzyme pockets, in addition to excellent binding manner with the active site of the target macromolecules (RNA-dependent RNA polymerase, Spike protein S1 and main protease) in relation to some broad-spectrum antiviral agents. Menoufia propolis could be a promising candidate in the combat against the pandemic COVID-19.

show abstract

Antimicrobial activity of propolis and gentamycin against methicillin-resistant Staphylococcus aureus in a 3D thermo-sensitive hydrogel

Cited by 14 publications

References 44 publications

Synthesis of an injectable heparin conjugated poloxamer hydrogel with high elastic recoverability for temporomandibular joint disorders

Synthesis of an injectable heparin conjugated poloxamer hydrogel with high elastic recoverability for temporomandibular joint disorders

Design and evaluation of chitosan-amino acid thermosensitive hydrogel

Potential anti-COVID-19 Activity of Egyptian Propolis Using Computational Modeling

Contact Info

Product

Resources

About