In vitro Activity of Piperacillin/Tazobactam against 615 &lt;i&gt;Pseudomonas aeruginosa&lt;/i&gt; Strains Isolated in Intensive Care Units

Bonfiglio, Giovanni; Laksai, Younes; Franceschini, Nicola; Perilli, Mariagrazia; Segatore, Bernardetta; Bianchi, Ciro; Stefani, Stefania; Amicosante, Gianfranco; Nicoletti, Giuseppe

doi:10.1159/000007127

Cited by 11 publications

(3 citation statements)

References 20 publications

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“…OXA-type β-lactamase belonging to molecular classes D, PER and VEB predominantly occur in P. aeruginosa and appears to originate from Turkey and France [8,9,10,11]. In P. aeruginosa , OXA-type β-lactamase is responsible for a high level of resistance to carboxypenicillins, ureidopenicillins and cephalosporins, and their activities are weakly inhibited by clavulanic acid, while PER and VEB β-lactamase also confer high-level resistance to ceftazidime, cefotaxime and aztreonam, which is reversed by clavulanic acid [6,12,13]. …”

Section: Introductionmentioning

confidence: 99%

First Detection of OXA-10 Extended-Spectrum Beta-Lactamases and the Occurrence of mexR and nfxB in Clinical Isolates of Pseudomonas aeruginosa from Nigeria

Odumosu¹,

Adeniyi

Chandra

2015

Chemotherapy

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Background: The characterization of β-lactamase production in Pseudomonasaeruginosa is rarely reported in Nigeria. The objective of this study was to investigate the occurrence and characterize the different β-lactamases as well as mechanisms of fluoroquinolones resistance among P. aeruginosa isolated from various clinical sources from Nigeria. Materials and Method: Isolates were investigated using PCR, RFLP and sequencing for the detection of various β-lactamases and efflux pump regulator genes. Result: The prevalence of OXA-10, AmpC, CTX-M and SHV in P. aeruginosa was 80, 70, 5 and 5%, respectively. The coexistence of bla_OXA-10 with bla_AmpC, bla_SHV and bla_CTX-M was reported in 40, 5 and 5% of isolates, respectively. The efflux pump regulator genes mexR and nfxB were both amplified in 45% of the OXA-10-positive isolates. Conclusion: This is the first report of the characterization of OXA-10 extended-spectrum β-lactamases and occurrence of mexR and nfxB efflux regulator genes in clinical isolates of P. aeruginosa in Nigeria.

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Section: Introductionmentioning

confidence: 99%

First Detection of OXA-10 Extended-Spectrum Beta-Lactamases and the Occurrence of mexR and nfxB in Clinical Isolates of Pseudomonas aeruginosa from Nigeria

Odumosu¹,

Adeniyi

Chandra

2015

Chemotherapy

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“…Tazobactam is an excellent suicide β-lactamase-inhibitor (Livermore et al, 1992;Perilli et al, 1999). Piperacillin in association to tazobactam showed a very good activity against P. aeruginosa resistant strains isolated in intensive care units (Bonfiglio et al, 1998a).…”

Section: Introductionmentioning

confidence: 99%

Synergistic effect of polyethylenimine plus piperacillin/tazobactam on clinicalPseudomonas aeruginosa isolates resistant to piperacillin/tazobactam

Bonfiglio

2006

Ann. Microbiol.

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We investigated the in vitro susceptibility of piperacillin/tazobactam in association with polyethylenimine (PEI), a synthetic polycation polymer, against 24 clinical isolates of Psedomonas aeruginosa resistant to piperacillin/tazobactam. The strains were isolated from patients with respiratory tract infections. MIC of piperacillin/tazobactam was determined by agar dilution method in accordance with NCCLS methodology. An inoculum of 10 4 CFU/spot with or without PEI (250 nM final concentration) was used. Killing curves were performed for 3 piperacillin/tazobactam resistant strains chosen for their different range of MIC values to piperacillin/tazobactam (128, 256 and 512 mg/L, respectively). Analyses were performed in duplicate using a concentration of antibiotic of 16 mg/L. The addition of PEI (250 nM) increased the susceptibility of piperacillin/tazobactam (from 8 to 32 folds) in all the strains tested. Moreover, in all the strains tested piperacillin/tazobactam in association with PEI showed a bactericidal activity before 6 h. Intrinsic resistance of P. aeruginosa affects susceptibility to penicillins and is believed to entail broad spectrum impermeability. Different theories suggest that some degree of structure or organization exists in the periplasm of P. aeruginosa and that this modulates both the level of intrinsic, impermeability-determined resistance expressed by a strain and the efficiency with which the β-lactamase can contact and destroy the incoming β-lactamase molecules. The explanation of this behaviour could be that PEI determines an increased permeability of the outer membrane of P. aeruginosa; tazobactam inhibits β-lactamase allowing the antibacterial activity of piperacillin. In conclusion our data suggest that PEI can be expected to act as a vehicle for experimental drug delivery into cells, and as a potentiating agent for antibacterial agents that are normally excluded by Gram-negative bacteria.

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“…There are three classes of antibiotic resistance in P. Aeruginosa: Intrinsic resistance, acquired resistance and genetic resistance (10). Another part of the resistance appears to be caused by two recently discovered multidrug efflux systems (11). In some cases, enzymes that specifically inactivate antibiotics lead to antibiotic resistance.…”

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Activity of isolated specific bacteriophage in treatment of chronic osteomyelitis induced by multiple drug resistance Pseudomonas aeruginosa in Rabbits

Sarhan

2018

Iraqi J. Vet. Med.

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This study was conducted to find out the possibility of using the Pseudomonas aeruginosa specific-bacteriophage as an alternative to antibiotics in treatment of chronic osteomyelitis in rabbits by injection of Pseudomonas aeruginosa suspension in tibia. The current study included an isolation of bacteriophage from sewage water by using agar overlay method and also an isolation of the bacteria from patients suffering from post-traumatic bone infection. The second experiment was in-vivo evaluation of phage activity in treatment of chronic osteomyelitis in rabbits. All animals of infected groups with Pseudomonas aeruginosa before treatment exhibited histopathological changes after 35 days of infection, the infected groups showed chronic osteomyelitis represented by sever chronic inflammatory cells infiltrates mainly lymphocytes, and macrophages and hemorrhage between bone trabeculae, also some sections showed extensive fibrosis in the marrow spaces. The treated group with P. aeruginosa specific – bacteriophage (1.5 ×107) PFU/ml for 10 days showed early repair elucidated by presence whorls of chondrocytes, and also the presence of multiple osteoblasts indicated bone formations. Also the presence of extensive fibrosis in the marrow space with present of osteoblasts indicated bone formations and repair.

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In vitro Activity of Piperacillin/Tazobactam against 615 <i>Pseudomonas aeruginosa</i> Strains Isolated in Intensive Care Units

Cited by 11 publications

References 20 publications

First Detection of OXA-10 Extended-Spectrum Beta-Lactamases and the Occurrence of <b><i>mexR </i></b>and <b><i>nfxB</i></b> in Clinical Isolates of <b><i>Pseudomonas aeruginosa</i></b> from Nigeria

First Detection of OXA-10 Extended-Spectrum Beta-Lactamases and the Occurrence of <b><i>mexR </i></b>and <b><i>nfxB</i></b> in Clinical Isolates of <b><i>Pseudomonas aeruginosa</i></b> from Nigeria

Synergistic effect of polyethylenimine plus piperacillin/tazobactam on clinicalPseudomonas aeruginosa isolates resistant to piperacillin/tazobactam

Activity of isolated specific bacteriophage in treatment of chronic osteomyelitis induced by multiple drug resistance Pseudomonas aeruginosa in Rabbits

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