In vitro activity of essential oils against Pseudomonas aeruginosa isolated from infected hip implants

Amorese, Veronica; Donadu, Matthew Gavino; Usai, Donatella; Sanna, A.; Milia, F.; Pisanu, Francesco; Molicotti, Paola; Zanetti, Stefania Anna Lucia; Doria, Carlo

doi:10.3855/jidc.10988

Cited by 30 publications

(24 citation statements)

References 15 publications

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“…For example, essential oils can inhibit bacteria in various approaches including triggering the degradation of bacterial cell wall, leakage of cell contents, membrane protein damage, disruption of cytoplasmic membrane, depletion of the proton motive force or coagulation of cytoplasm [30]. Additionally, the hydrophobicity of essential oils enables the permeation of essential oils into cell membrane, and thus facilitating the spillage of ions and molecules out of the cells and cell apoptosis occurs [33]. In our study, the antimicrobial activities of the essential oil could potentially stem from their main components such as linalool and E-β-caryophyllene as their antimicrobial potentials were well-reported Table 4.…”

Section: Discussionmentioning

confidence: 99%

“…Bacteria, fungi and parasites have consecutively been developing numerous resistant mechanisms against current antibiotics, hampering the success of anti-infectious therapies, and thus leaving severe consequences on patients' health [30,[31][32][33][34]. In addition, the use of synthetic chemicals to control microorganisms is still limited due to their carcinogenic effects, acute toxicity and environmental hazards [32].…”

Section: Discussionmentioning

confidence: 99%

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Biological activities of essential oil extracted from leaves of Atalantia sessiflora Guillauminin Vietnam

Donadu

et al. 2020

J Infect Dev Ctries

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Introduction: The present study aimed to determine the chemical compositions and bioactivities of the essential oil of Atalantia sessiflora Guillaumin (A. sessiflora), including antibacterial, antimycotic, antitrichomonas, anti-inflammatory and antiviral effects. Methodology: The essential oil from leaves of A. sessiflora was extracted by hydrodistillation using a Clevenger apparatus. Chemical compositions of oil were identified by GC/MS. Antimicrobial and antitrichomonas activity were determined by the microdilution method; anti-inflammatory and antiviral were determined by the MTT method. Results: The average yield of oil was 0.46 ± 0.01% (v/w, dry leaves). A number of 45 constituents were identified by GC/MS. The essential oil comprised four main components. The oil showed antimicrobial activities against Gram-positive strains as Staphylococcus; Gram-negative bacteria such as Klebsiella pneumoniae and Escherichia coli; and finally four Candida species. Enterococcus faecalis and Pseudomonas aeruginosa were least susceptible to the oil of A. sessiflora, as seen in their MIC and MLC values over 16% (v/v). Activity against Trichomonas vaginalis was also undertaken, showing IC50, IC90 and MLC values of 0.016, 0.03 and 0.06% (v/v) respectively, after 48 hours of incubation. The oil of A. sessiflora displayed activity against the nitric oxide generation with the IC50 of 95.94 ± 6.18 µg/mL. The oil was completely ineffective against tested viruses, ssRNA+, ssRNA-, dsRNA, and dsDNA viruses. Conclusions: This is the first yet comprehensive scientific report about the chemical compositions and pharmacological properties of the essential oil of A. sessiflora. Further studies should be done to evaluate the safety and toxicity of A. sessiflora oil.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Biological activities of essential oil extracted from leaves of Atalantia sessiflora Guillauminin Vietnam

Donadu

et al. 2020

J Infect Dev Ctries

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“…Additionally, it is questionable how long the new agents can manage the worsening resistance situation [146,157,[249][250][251][252][253][254]. With the daunting increase in antimicrobial resistance rates in all types of bacteria, one of the main aims of antimicrobial research is the exploration for new approaches past conventional antibiotics, such as bacteriophages, antimicrobial peptides with diverse structures and mechanisms of action, virulence inhibitors, siderophores, compounds from natural origins (like essential oils), and other adjuvants (e.g., efflux pump inhibitors, monoclonal antibodies) (Table 3) [118,[255][256][257][258][259][260][261][262][263][264][265][266][267][268][269][270]. It is possible that in the next couple of decades, these agents will play a major role in the management of serious bacterial infections caused by P. aeruginosa and other pathogens of critical importance.…”

Section: Emerging Therapeutic Options For Pseudomonas Infectionsmentioning

confidence: 99%

It’s Not Easy Being Green: A Narrative Review on the Microbiology, Virulence and Therapeutic Prospects of Multidrug-Resistant Pseudomonas aeruginosa

2021

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Pseudomonas aeruginosa is the most frequent cause of infection among non-fermenting Gram-negative bacteria, predominantly affecting immunocompromised patients, but its pathogenic role should not be disregarded in immunocompetent patients. These pathogens present a concerning therapeutic challenge to clinicians, both in community and in hospital settings, due to their increasing prevalence of resistance, and this may lead to prolonged therapy, sequelae, and excess mortality in the affected patient population. The resistance mechanisms of P. aeruginosa may be classified into intrinsic and acquired resistance mechanisms. These mechanisms lead to occurrence of resistant strains against important antibiotics—relevant in the treatment of P. aeruginosa infections—such as β-lactams, quinolones, aminoglycosides, and colistin. The occurrence of a specific resistotype of P. aeruginosa, namely the emergence of carbapenem-resistant but cephalosporin-susceptible (Car-R/Ceph-S) strains, has received substantial attention from clinical microbiologists and infection control specialists; nevertheless, the available literature on this topic is still scarce. The aim of this present review paper is to provide a concise summary on the adaptability, virulence, and antibiotic resistance of P. aeruginosa to a readership of basic scientists and clinicians.

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“…The cytotoxic effect of plant oils in high-concentrations also has been reported in some studies, which lead to the limited application of these compounds as a therapeutic agent in Invivo conditions. 31,32 According to the results of this study and similar studies, it seems that the anti-biofilm effect of Curcumin in P. aeruginosa is more related to inhibition of secretion of different components of the biofilm to extracellular space of bacteria or direct disruption of biofilm stability due to reaction with its structural protein component rather than affecting the expression of regulatory genes related to QS and two-component regulatory systems that control biofilm formation in this bacterium. Further studies seem to be necessary to determine the exact mechanism of biofilm inhibition.…”

Section: Discussionmentioning

confidence: 52%

<p>Investigating the Effect of Nano-Curcumin on the Expression of Biofilm Regulatory Genes of <em>Pseudomonas aeruginosa</em></p>

Sharifian¹,

Yaslianifard²,

Fallah³

et al. 2020

IDR

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Background: Pseudomonas aeruginosa is an opportunistic pathogen that causes serious nosocomial infections, especially in immunodeficient patients and cystic fibrosis, cancer, and burned individuals. The biofilm that plays an important role in the virulence of P. aeruginosa is under the regulation of quorum sensing and two-component regulatory systems of bacteria. Curcumin, an active phenolic extract of turmeric has shown an inhibitory effect on the biofilm formation of some pathogenic bacteria. Thus, the present study aims to evaluate the effect of Nano-Curcumin on the expression of major regulatory genes involved in biofilm formation of P. aeruginosa. Materials and Methods: The biofilm formation of P. aeruginosa ATCC 10145 was assessed in the presence of 15, 20, and 25 µg/mL concentrations of Nano-Curcumin using the microplate titer method. The effect of Nano-Curcumin on the expression level of regulatory genes were determined by relative reverse transcriptase-realtime PCR. Results: In the absence of Nano-Curcumin, P. aeruginosa strain ATCC 10145 strongly produced biofilm (3+) and in the presence of 15 and 20 µg/mL, biofilm formation was reduced to moderate (2+) and weak biofilm producer (1+), respectively. Nano-Curcumin at a concentration of 25µg/mL inhibited biofilm formation in P. aeruginosa. The expression of regulatory genes was not affected by biofilm inhibitory concentrations of Nano-Curcumin. Conclusion: The antibiofilm mechanism of Curcumin is not related to the downregulation of regulatory systems of P. aeruginosa and probably it prevents the formation of a complete biofilm structure.

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In vitro activity of essential oils against Pseudomonas aeruginosa isolated from infected hip implants

Cited by 30 publications

References 15 publications

Biological activities of essential oil extracted from leaves of Atalantia sessiflora Guillauminin Vietnam

Biological activities of essential oil extracted from leaves of Atalantia sessiflora Guillauminin Vietnam

It’s Not Easy Being Green: A Narrative Review on the Microbiology, Virulence and Therapeutic Prospects of Multidrug-Resistant Pseudomonas aeruginosa

<p>Investigating the Effect of Nano-Curcumin on the Expression of Biofilm Regulatory Genes of <em>Pseudomonas aeruginosa</em></p>

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