In Vitro Activities of Six Antifungal Drugs Against Candida glabrata Isolates: An Emerging Pathogen

Amirrajab, Nasrin; Badali, Hamid; Didehdar, Mojtaba; Afsarian, Mohammad Hosein; Mohammadi, Rasoul; Lotfi, Nazanin; Shokohi, Tahereh

doi:10.5812/jjm.36638

Cited by 11 publications

(7 citation statements)

References 39 publications

(60 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results revealed that the highest resistance rate (13%) was associated with itraconazole, which is consistent with the studies of Shokohi et al (2011) (12.5%) and Espinel-Ingroff et al (2005) (17%). On the other hand, our results are inconsistent with those of Richter et al (2005) and Amirrajab et al (2016) who observed a resistance of up to 70% to itraconazole. This difference may be due to the difference in the source of samples and less prescription of antifungal drugs for the treatment of vaginal infections.…”

Section: Discussioncontrasting

confidence: 99%

Microsatellite Typing and Antifungal Susceptibility of Candida glabrata Strains Isolated From Patients With Candida Vaginitis

2019

View full text Add to dashboard Cite

Vulvovaginal candidiasis (VVC) is a yeast infection with a global reach and millions of dollars are spent annually for its diagnosis and treatment. Recently, Candida glabrata with different degrees of antifungal resistance has been considered as the second most common cause of vaginal infections. The aim of the present study is to determine the antifungal susceptibility and molecular epidemiology profiles of C. glabrata isolates from patients with VVC. Sixty-one C. glabrata isolates were examined for antifungal susceptibility using the EUCAST broth microdilution method. Moreover, microsatellite length polymorphism (MLP) was used for typing the C. glabrata isolates using six microsatellite markers. Overall, 13, 3.3, and 0% of the isolates were non-wild types to itraconazole, posaconazole, and voriconazole, respectively. Sixty (98.4%) isolates were an intermediate phenotype to fluconazole and only one isolate was fluconazole resistant. Microsatellite length polymorphism with a discriminatory power of 0.964 identified 35 distinct types and 24 singleton genotypes. The assessment of the population genetic structure revealed that the non-wild-type population had a moderate genetic differentiation compared to the wild type population (F ST = 0.1457). It was also found that the most common genotypes were G27 (eight strains), G12 (six strains), and G4 (five strains). We found that eight strains were resistant/a non-wild phenotype to itraconazole. Five out of eight (62.5%) resistant/non-wild phenotype strains correlated to a predominant genotype (GT27) and the rest belonged to GT11 (12.5%), GT29 (12.5%), and GT28 (12.5%). The current study is the first molecular epidemiology study in the southwest of Iran and demonstrates the antifungal susceptibility profiles of C. glabrata in it. This study shows a wide range of the genetic diversity of C. glabrata (35 different genotypes) from VVC in the southwest of Iran. The majority of the non-wild isolates had a dominant genotype or genotypes related to this dominant genotype (clonal cluster one).

show abstract

Section: Discussioncontrasting

confidence: 99%

Microsatellite Typing and Antifungal Susceptibility of Candida glabrata Strains Isolated From Patients With Candida Vaginitis

2019

View full text Add to dashboard Cite

show abstract

“…As mentioned earlier, the samples had been collected in previous studies (references were cited) and were only molecularly confirmed in this study ( Figure 2 ). In the present research, based on the EUCAST guideline, the highest resistance rate to amphotericin B (20%) was observed at the MIC range of 0.25-4 µg/mL, MIC 90 of 4µg/mL, MIC 50 of 0.5 µg/mL, and geometric mean MIC of 0.44 µg/mL, which is similar to the previous reports [ 35 , 36 ]. This study indicated that 6.7% of the isolates were resistant (non-wild type) to posaconazole with an MIC range of 0.032-4 µg/mL, which is consistent with the results obtained by Badie et al .…”

Section: Discussionsupporting

confidence: 91%

“…Based on the results of the current study, caspofungin was an effective drug tested against C. glabrata with the MIC range of 0.032-1 µg/mL, MIC 90 of 0.5 µg/mL, MIC 50 of 0.5 µg/mL, and geometric mean MIC of 0.44 µg/mL that is in agreement with the results reported by Labbe et al . and other researchers [ 35 , 37 , 45 , 46 ]. Our study showed that MIC 90 values for fluconazole, posaconazole, and voriconazole were significantly lower than those obtained by Espinel-Ingroff et al .…”

Section: Discussionmentioning

confidence: 93%

See 1 more Smart Citation

In vitro antifungal susceptibilities of six antifungal drugs against clinical Candida glabrata isolates according to EUCAST

Fatahinia

Halvaeizadeh

Mahmoudabadi

et al. 2020

CMM

View full text Add to dashboard Cite

Background and Purpose: Candida glabrata is the second cause of candidiasis. The mortality rate of C. glabrata infections is about 40%; accordingly, it may be life threatening, especially in immunocompromised hosts. Regarding this, the current study was conducted to evaluate the regional patterns of the antifungal susceptibility of clinical C. glabrata isolated from the patients referring to the health centers located in Ahvaz, Iran. Materials and Methods: In this study, a total of 30 clinical strains of C. glabrata isolates were recovered from different body sites (i.e., vagina, mouth, and urine). Phenotypic characteristics and molecular methods were used to identify the isolates. The minimum inhibitory concentration (MIC) was determined according to the European Committee on Antimicrobial Susceptibility Testing. Results: Our findings demonstrated that 20%, 80%, and 6.7% of the isolates were resistant to amphotericin B, terbinafine, and posaconazole, respectively, while all the isolates were found to be fluconazole susceptible dose dependent and susceptible to voriconazole and caspofungin. Conclusion: Our study suggested that voriconazole had high potency against C. glabrata isolates. Consequently, this antifungal agent can be an alternative drug in the treatment of resistant patients. These results can be helpful for the successful treatment of patients in different regions.

show abstract

“…In our previous study performed in Iran, the resistance rates of C. albicans strains to fluconazole, itraconazole, and voriconazole were obtained as 9.1%, 11.3% and 9.1%, respectively [35]. In another study, we reported fluconazole, itraconazole, and voriconazole resistance rates of 10%, 72.5%, and 37.5% for C. glabrata isolates, respectively [36].…”

Section: Discussionmentioning

confidence: 97%

In vitro activity of four triazole antifungal drugs against clinically common and uncommon yeast species

Aslani¹,

Shokohi²,

Ataollahi³

et al. 2019

CMM

Self Cite

View full text Add to dashboard Cite

Background and Purpose: Incidence of fungal infections caused by opportunistic fungal pathogens, such as yeasts and yeast-like species, has undergone an increase in otherwise healthy individuals. These pathogens account for high mortality and show reduced susceptibility to the routine antifungal drugs. Accordingly, antifungal susceptibility testing is an urgent need in the determination of the susceptibility spectrum of antifungals and selection of appropriate antifungal agents for the management of patients with fungal infection.Materials and Methods: The present study was conducted on 110 yeast strains belonging to 15 species recovered from clinical specimens. Susceptibility of the isolates to four antifungal drugs (i.e., fluconazole, itraconazole, voriconazole, and posaconazole) was tested according to the Clinical and Laboratory Standards Institute guidelines M27-A3 and M27-S4.Results: Fluconazole exhibited no activity against 4.3% (n=2) of C. albicans isolates, whereas the remaining 44 isolates had a minimum inhibitory concentration (MIC) range of 0.125-4 μg/ml. Voriconazole had the lowest geometric mean MIC (0.03 μg/ml) against all isolated yeast species, followed by posaconazole (0.07 μg/ml), itraconazole (0.10 μg/ml), and fluconazole (0.60 μg/ml). Overall, all of the isolates had reduced voriconazole MICs with a MIC range of 0.016-0.5 μg/ml, except for one isolate of C. albicans that had a MIC of 1 μg/ml. Candida haemulonii as a multidrug-resistant fungus showed a fluconazole MIC of > 64 μg/ml.Conclusion: The current study provides insight into the antifungal susceptibility profiles of clinically common and uncommon yeast species to four triazole antifungal agents. According to our findings, voriconazole was the most active agent. Awareness about antifungal susceptibility patterns is highly helpful in the selection of appropriate antifungal drugs and identification of the efficiency of the currently used agents.

show abstract

In Vitro Activities of Six Antifungal Drugs Against Candida glabrata Isolates: An Emerging Pathogen

Cited by 11 publications

References 39 publications

Microsatellite Typing and Antifungal Susceptibility of Candida glabrata Strains Isolated From Patients With Candida Vaginitis

Microsatellite Typing and Antifungal Susceptibility of Candida glabrata Strains Isolated From Patients With Candida Vaginitis

In vitro antifungal susceptibilities of six antifungal drugs against clinical Candida glabrata isolates according to EUCAST

In vitro activity of four triazole antifungal drugs against clinically common and uncommon yeast species

Contact Info

Product

Resources

About