In Vitro Activities of Fluoroquinolones Entrapped in Non-ionic Surfactant Vesicles against Ciprofloxacin-Resistant Bacteria Strains

Adebayo, Amusa Sarafadeen; Jankie, Satish; Pillai, Gopalakrishna

doi:10.7243/2050-120x-1-5

Cited by 26 publications

(28 citation statements)

References 12 publications

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“…32 Two ciprofloxacin-loaded niosomal preparations were tested; preparation II had an enhanced antimicrobial activity against ciprofloxacin resistant clinical isolates compared to preparation I. Encapsulation of ciprofloxacin, in niosomal preparation II, reduced its MIC by 8-32-fold in most isolates, and caused reversal of resistance phenotype in three isolates. A previous study by Satish et al 13 has recorded a reduction of only 2-4-fold in ciprofloxacin MIC, by niosomal formulations tested on ciprofloxacin resistant isolates.…”

Section: Discussionmentioning

confidence: 88%

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The Antimicrobial Activity of Ciprofloxacin-Loaded Niosomes against Ciprofloxacin-Resistant and Biofilm-Forming Staphylococcus aureus

Kashef

Saleh

Assar

et al. 2020

IDR

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The threat of Staphylococcus aureus antimicrobial resistance is increasing worldwide. Niosomes are a new drug delivery system that enhances the antimicrobial potential of antibiotics. We hereby aim to evaluate the antimicrobial and antibiofilm activity of ciprofloxacin-loaded niosomes. Methods: The antimicrobial susceptibility of clinical S. aureus isolates (n=59) was determined by Kirby-Bauer disk diffusion method. Their biofilm formation activity was tested by Christensen's method. Two ciprofloxacin-loaded niosomal formulations were prepared by thin-film hydration method, and their minimum inhibitory concentrations (MIC) were determined by agar dilution method, against ciprofloxacin-resistant and biofilm-forming isolates (n=24). Their ability to inhibit biofilm formation and eradicate already formed biofilms was evaluated and further confirmed by scanning electron microscope images. Non-synonymous mutations, in a quinolone resistance-determining regions of S. aureus isolates, were detected by polymerase chain reaction. Results: Most of the isolates were methicillin-(47/59) and ciprofloxacin-resistant (45/59). All except two isolates were capable of biofilm production. Niosomal preparation I reduced ciprofloxacin MIC by twofold in four isolates, whereas preparation II reduced ciprofloxacin MIC of most isolates by 8-to 32-fold, with three isolates that became ciprofloxacinsusceptible. Non-synonymous mutations were detected in isolates that maintained phenotypic ciprofloxacin resistance against ciprofloxacin-loaded niosomal preparation II. Ciprofloxacinloaded niosomes reduced the minimum biofilm inhibitory concentration and the minimum biofilm eradication concentration in 58% and 62% of the tested isolates, respectively. Conclusion: Ciprofloxacin-loaded niosomes can restore ciprofloxacin activity against resistant S. aureus isolates. To our knowledge, this is the first report on the inhibition of biofilm formation and eradication of formed biofilms by ciprofloxacin-loaded niosomes.

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Section: Discussionmentioning

confidence: 88%

“…The EE% was calculated with reference to total amount of ciprofloxacin added during the preparation. 13…”

Section: Entrapment Efficiency (Ee)mentioning

confidence: 99%

The Antimicrobial Activity of Ciprofloxacin-Loaded Niosomes against Ciprofloxacin-Resistant and Biofilm-Forming Staphylococcus aureus

Kashef

Saleh

Assar

et al. 2020

IDR

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“…Results indicated potentials of VCM-eluting niosomes as an effective antimicrobial delivery system. Enhancement of antimicrobial activity by niosomal entrapment has been attributed to interaction between niosomes and microorganisms (24,26). As a vesicular system, niosomes may undergo fusion with the outer membrane of organisms, eliciting increased fluidity (24,60) and enhancing permeability of the drug released in the vicinity.…”

Section: Minimal Inhibitory Concentrations (Mics)mentioning

confidence: 99%

“…As a vesicular system, niosomes may undergo fusion with the outer membrane of organisms, eliciting increased fluidity (24,60) and enhancing permeability of the drug released in the vicinity. Niosome encapsulation was also reported to enhance the antimicrobial activity of other antibiotics such as fluoroquinolones (24,25,61).…”

Section: Minimal Inhibitory Concentrations (Mics)mentioning

confidence: 99%

“…Being more physicochemically stable, easily handled, and less expensive, niosomes overcome the main limitations of liposomes. Niosomes are recognized to control the release and enhance the activity of antimicrobial agents including antibiotics (24,25), antibacterial, and antifungal agents (26)(27)(28) and to interact with phospholipid membranes (29). The use of hydrophilic polyethoxylated surfactants such as Tweens and Brijs either alone (30,31) or in combination with lipophilic surfactants (cosurfactant niosomes) (32) confers bilayer hydrophilicity which enhances entrapment of hydrophilic drugs and may prevent bacterial adhesion at niosome-treated surfaces.…”

Section: Introductionmentioning

confidence: 99%

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Vancomycin-Eluting Niosomes: A New Approach to the Inhibition of Staphylococcal Biofilm on Abiotic Surfaces

et al. 2014

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Abstract. A new vancomycin (VCM)-eluting mixed bilayer niosome formulation was evaluated for the control of staphylococcal colonization and biofilm formation on abiotic surfaces, a niosome application not explored to date. Cosurfactant niosomes were prepared using a Span 60/Tween 40/cholesterol blend (1: 1: 2). Tween 40, a polyethoxylated amphiphile, was included to enhance VCM entrapment and confer niosomal surface properties precluding bacterial adhesion. VCM-eluting niosomes showed good quality attributes including relatively high entrapment efficiency (∼50%), association of Tween 40 with vesicles in a constant proportion (∼87%), biphasic release profile suitable for inhibiting early bacterial colonization, and long-term stability at 4°C for a 12-month study period. Niosomes significantly enhanced VCM activity against planktonic bacteria of nine staphylococcal strains. Using microtiter plates as abiotic surface, VCM-eluting niosomes proved superior to VCM in inhibiting biofilm formation, eradicating surface-borne biofilms, inhibiting biofilm growth, and interfering with biofilm induction by VCM subminimal inhibitory concentrations. Data suggest dual functionality of cosurfactant VCM-eluting niosomes as passive colonization inhibiting barrier and active antimicrobial-controlled delivery system, two functions recognized in infection control of abiotic surfaces and medical devices.

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Molecular level understanding of resistance to nalidixic acid in Salmonella enteric serovar typhimurium associates with the S83F sequence type

Preethi

Ramanathan

2015

Eur Biophys J

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Nalidixic acid is an antibiotic drug used for treatment of Salmonellosis, a gastrointestinal infection. DNA gyrase subunit A (GyrA) of Salmonella typhimurium is the drug target for nalidixic acid. Resistance of GyrA to nalidixic acid, because of a point mutation in S. typhimurium, was recently reported. Substitution of Phe in place of Ser at locus 83 in GyrA of S. typhimurium has been experimentally associated with nalidixic acid resistance. Despite recent efforts, the mechanism of this resistance is not well understood. In this investigation we used computational techniques to address this shortcoming. Our results showed that contact with residue Arg 91 is certainly important for efficient binding of nalidixic acid to the target protein, and that mutation of this residue results in 180° rotation of the antibiotic in its binding pocket, around its own long axis. It is hoped these findings may enable development of new antibiotics against resistant forms of Salmonella.

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In Vitro Activities of Fluoroquinolones Entrapped in Non-ionic Surfactant Vesicles against Ciprofloxacin-Resistant Bacteria Strains

Cited by 26 publications

References 12 publications

<p>The Antimicrobial Activity of Ciprofloxacin-Loaded Niosomes against Ciprofloxacin-Resistant and Biofilm-Forming <em>Staphylococcus aureus</em></p>

<p>The Antimicrobial Activity of Ciprofloxacin-Loaded Niosomes against Ciprofloxacin-Resistant and Biofilm-Forming <em>Staphylococcus aureus</em></p>

Vancomycin-Eluting Niosomes: A New Approach to the Inhibition of Staphylococcal Biofilm on Abiotic Surfaces

Molecular level understanding of resistance to nalidixic acid in Salmonella enteric serovar typhimurium associates with the S83F sequence type

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