In Vitro Activation Early Follicles: From the Basic Science to the Clinical Perspectives

Vo, Kim; Kawamura, Kazuhiro

doi:10.3390/ijms22073785

Cited by 28 publications

(26 citation statements)

References 131 publications

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“…Notably, the presence of numerous reserve follicles in the ovaries of these patients may open the way to in vitro follicular maturation and treatment of their infertility in the future [119]. stage of secondary follicles and the formation of a functional theca interna is independen of FSH in mice.…”

Section: Lessons Derived From Studying a Complete Molecular Alteration Of The Human Fshr In Human Primary Ovarian Insufficiencymentioning

confidence: 99%

“…Notably, the presence of numerous reserve follicles in the ovaries of these patients may open the way to in vitro follicular maturation and treatment of their infertility in the future [119]. (E-G), primordial, intermediary and primary follicles of the control ovary labeled with anti-PCNA antibody.…”

Section: Lessons Derived From Studying a Complete Molecular Alteration Of The Human Fshr In Human Primary Ovarian Insufficiencymentioning

confidence: 99%

Section: The Role Of Fsh In the Human Ovary Revisitedmentioning

confidence: 99%

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The Roles of Luteinizing Hormone, Follicle-Stimulating Hormone and Testosterone in Spermatogenesis and Folliculogenesis Revisited

Oduwole

Huhtaniemi

Misrahi

2021

IJMS

128

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Spermatogenesis and folliculogenesis involve cell–cell interactions and gene expression orchestrated by luteinizing hormone (LH) and follicle-stimulating hormone (FSH). FSH regulates the proliferation and maturation of germ cells independently and in combination with LH. In humans, the requirement for high intratesticular testosterone (T) concentration in spermatogenesis remains both a dogma and an enigma, as it greatly exceeds the requirement for androgen receptor (AR) activation. Several data have challenged this dogma. Here we report our findings on a man with mutant LH beta subunit (LHβ) that markedly reduced T production to 1–2% of normal., but despite this minimal LH stimulation, T production by scarce mature Leydig cells was sufficient to initiate and maintain complete spermatogenesis. Also, in the LH receptor (LHR) knockout (LuRKO) mice, low-dose T supplementation was able to maintain spermatogenesis. In addition, in antiandrogen-treated LuRKO mice, devoid of T action, the transgenic expression of a constitutively activating follicle stimulating hormone receptor (FSHR) mutant was able to rescue spermatogenesis and fertility. Based on rodent models, it is believed that gonadotropin-dependent follicular growth begins at the antral stage, but models of FSHR inactivation in women contradict this claim. The complete loss of FSHR function results in the complete early blockage of folliculogenesis at the primary stage, with a high density of follicles of the prepubertal type. These results should prompt the reassessment of the role of gonadotropins in spermatogenesis, folliculogenesis and therapeutic applications in human hypogonadism and infertility.

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Section: Lessons Derived From Studying a Complete Molecular Alteration Of The Human Fshr In Human Primary Ovarian Insufficiencymentioning

confidence: 99%

“…Notably, the presence of numerous reserve follicles in the ovaries of these patients may open the way to in vitro follicular maturation and treatment of their infertility in the future [119]. (E-G), primordial, intermediary and primary follicles of the control ovary labeled with anti-PCNA antibody.…”

Section: Lessons Derived From Studying a Complete Molecular Alteration Of The Human Fshr In Human Primary Ovarian Insufficiencymentioning

confidence: 99%

See 1 more Smart Citation

The Roles of Luteinizing Hormone, Follicle-Stimulating Hormone and Testosterone in Spermatogenesis and Folliculogenesis Revisited

Oduwole

Huhtaniemi

Misrahi

2021

IJMS

128

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show abstract

“…Then, the cubes are incubated with a PTEN inhibitor or a PI3K stimulator for 2 days followed by autotransplantation beneath the serosa of the Fallopian tubes. The follicle growth is then stimulated by exogenous gonadotropin under suppression of luteinizing hormone to generate competent mature oocytes for subsequent IVF procedure [86]. So far, at least two live births in 20 POI patients have been reported after IVA [86,87].…”

Section: The Role Of Noncoding Rnas In Potential Treatment Of Poimentioning

confidence: 99%

“…The follicle growth is then stimulated by exogenous gonadotropin under suppression of luteinizing hormone to generate competent mature oocytes for subsequent IVF procedure [86]. So far, at least two live births in 20 POI patients have been reported after IVA [86,87]. Recently, the IVA procedure was modified into so-called "drug-free IVA" to avoid the need for two surgical procedures.…”

Section: The Role Of Noncoding Rnas In Potential Treatment Of Poimentioning

confidence: 99%

The Role of Noncoding RNA in the Pathophysiology and Treatment of Premature Ovarian Insufficiency

Pankiewicz¹,

Laudański

Issat³

2021

IJMS

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Premature ovarian insufficiency (POI) is defined as a loss of ovarian function before the age of 40 years, with a prevalence rate estimated at approximately 1%. It causes infertility and is related to serious long-term health consequences, including reduced life expectancy, increased cardiovascular risk, decreased bone mineral density and neurological disorders. There is currently no effective therapy for POI that is widely available in clinical practice; therefore, the treatment of patients with POI is based on hormone replacement therapy. One of the recent advances in the understanding of the pathophysiology of POI has been the role of microRNAs (miRNAs) and other noncoding RNAs (ncRNAs) in the disease. Moreover, intensive research on human folliculogenesis and reproductive biology has led to the development of novel promising therapeutic strategies with the use of exosomal miRNAs derived from mesenchymal stem cells to restore ovarian function in POI patients. This narrative review focuses on the new studies concerning the role of ncRNAs in the pathogenesis of POI, together with their potential as biomarkers of the disease and targets for therapy.

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Immunolocalization of melatonin receptor type 1 in the sheep ovary and involvement of the PI3K/Akt/FOXO3a signaling pathway in the effects of melatonin on survival and in vitro activation of primordial follicles

Barberino

Macedo

Lins

et al. 2022

Molecular Reproduction Devel

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This study characterized the expression of melatonin receptor type 1 (MT 1 ) protein in sheep ovaries, evaluated melatonin effects on primordial follicle survival and development after in vitro culture of ovarian tissue and verified the possible involvement of the phosphatidylinositol-3-kinase/protein kinase B/forkhead box O3a (PI3K/Akt/FOXO3a) pathway in the melatonin actions. Ovine ovarian fragments were cultured in α-modified minimum essential medium alone (α-MEM + ) or supplemented with 100, 500, or 1000 pg/ml melatonin for 7 days. PI3K inhibition was performed through pretreatment of ovarian fragments with LY294002. Thereafter, immunohistochemistry was performed to evaluate the expression of cleaved caspase-3, Akt, phosphorylated-Akt, and phosphorylated-FOXO3a (p-FOXO3a). The immunohistochemical localization of the MT 1 receptor protein was documented in sheep preantral and antral follicles. After in vitro culture, 100 pg/ml melatonin showed higher follicular survival and activation than α-MEM + and other melatonin concentrations.After PI3K inhibition, there was an increase in cleaved caspase-3-positive follicles, and a decrease in the primordial follicle activation, Akt phosphorylation, and nuclear exclusion of p-FOXO3a. In conclusion, MT 1 receptor protein is present in the sheep ovary.Furthermore, 100 pg/ml melatonin maintains survival and stimulates activation of primordial follicles through the PI3K/Akt/FOXO3a signaling pathway after in vitro culture of sheep ovarian tissue. K E Y W O R D S hormone receptor, immunohistochemistry, in vitro culture, ovarian cortex, preantral follicle 1 | INTRODUCTION In vitro activation of primordial follicles cultured within the ovary itself is an essential tool to study the early follicle development and optimize the use of the female germ cell pool (Telfer & Zelinski, 2013; J. J. Wang et al., 2017). Studies on in vitro activation of primordial follicles, and subsequent growth and maturation have gained a lot of attention as a potential source of mature eggs for in vitro fertilization and embryo transfer, followed by the delivery of healthy born

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In Vitro Activation Early Follicles: From the Basic Science to the Clinical Perspectives

Cited by 28 publications

References 131 publications

The Roles of Luteinizing Hormone, Follicle-Stimulating Hormone and Testosterone in Spermatogenesis and Folliculogenesis Revisited

The Roles of Luteinizing Hormone, Follicle-Stimulating Hormone and Testosterone in Spermatogenesis and Folliculogenesis Revisited

The Role of Noncoding RNA in the Pathophysiology and Treatment of Premature Ovarian Insufficiency

Immunolocalization of melatonin receptor type 1 in the sheep ovary and involvement of the PI3K/Akt/FOXO3a signaling pathway in the effects of melatonin on survival and in vitro activation of primordial follicles

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