Anthocyanins biosynthesis
is a well-studied biosynthesis pathway
in Daucus carota. However, the scale-up
production at the bioreactor level and transporter involved in accumulation
is poorly understood. To increase anthocyanin content and elucidate
the molecular mechanism involved in accumulation, we examined D. carota cell culture in flask and bioreactor for
18 days under salt stress (20.0 mM NH4NO3/37.6
mM KNO3) at 3 day intervals. The expression of anthocyanin
biosynthesis and putative MATE (multidrug and toxic
compound extrusion) transporter expression was analyzed by qRT-PCR.
It was observed that there was a significant enhancement of anthocyanin
in the bioreactor compared to the control culture. A correlation was
observed between the expression of MATE and the anthocyanin
biosynthesis genes (CHS, C4H, LDOX, and UFGT) on the 9th day in a bioreactor,
where maximum anthocyanin accumulation and expression was detected.
We hypothesize the involvement of MATE in transporting
anthocyanin to tonoplast in D. carota culture under salt stress.