Salt Stress-Induced Anthocyanin Biosynthesis Genes and <i>MATE</i> Transporter Involved in Anthocyanin Accumulation in <i>Daucus carota</i> Cell Culture

Saad, Kirti R.; Kumar, Gyanendra; Mudliar, Sandeep N.; Giridhar, P.; Shetty, Nandini P.

doi:10.1021/acsomega.1c02941

Cited by 15 publications

(8 citation statements)

References 46 publications

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“…Increasing the amount of salt did not affect the cultivars with respect to the number of leaves in Tarwali and Hamanwali. The present finding is similar to that of the previous study [31]. Salt stress significantly reduces the leaf length in the cultivars Haleemi, Dhakki, Sanduri, Saghoi, and Hamanwali.…”

Section: Discussionsupporting

confidence: 93%

Characterization of Salt-Tolerant Cultivars of Date Palm Based on Morphological and Biochemical Responses Under Salinity Stress

Ali,

Ashraf,

Ahmed

et al. 2024

Pol. J. Environ. Stud.

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Abiotic stress, especially salinization, is considered a major soil problem in arid and semiarid regions. To combat salinization, halophytes such as date palms are grown in these areas. However, less information is available on the morphological and biochemical responses of different date palm cultivars under high salinity. In this regard, eight cultivars of date palm were selected and treated with different salinity levels to check the adaptive capabilities of these cultivars against salt stress in terms of morphological and biochemical attributes. The objective of the current study was to screen these cultivars for tolerance or susceptibility to salt stress (0, 50, 100, and 200 mM NaCl). The results of the morphological parameters revealed the negative impact of salt stress on the morphology. Higher concentrations of salt reduced the plant height (Haleemi, Dahakki, Sanduri, Saghoi, Tarwali, and Hamanwali), the number of leaves (Haleemi, Basrawali, Dhakki, Sanduri, Saghoi, and Gajjar),

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Section: Discussionsupporting

confidence: 93%

Characterization of Salt-Tolerant Cultivars of Date Palm Based on Morphological and Biochemical Responses Under Salinity Stress

Ali,

Ashraf,

Ahmed

et al. 2024

Pol. J. Environ. Stud.

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“…Our recent studies have noticed the importance of the MATE transporter in the anthocyanin accumulation during salt stress in D. carota (Saad et al 2021). The present work focused on screening the MATE gene family through genome-wide analysis and identifying the MATE involved in anthocyanin transport in in vitro cultures of D. carota.…”

Section: Discussionmentioning

confidence: 99%

“…Different gene-speci c primers were taken from the previously published paper and used to quantify the transcript by qPCR (Saad et al 2021). The qPCR was carried out in triplicates using a Quant studio 5 (Applied Biosystem, India) and the SYBR Green (Takara, India).…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

“…Based on the previous studies, we had identi ed the MATE gene (DcMATE21), which showed signi cant expression among other MATE under salt stress (Saad et al 2021). The Sanger sequencing analysis of cloned putative DcMATE21 (Accession number: MG682560) revealed an open reading frame (ORF) of 1536 bp, which encoded a predicted protein of 511 amino acids with a theoretical molecular weight of 56.14 kDa and pI value of 7.52.…”

Section: Cloning Of Full-length Dcmate From In Vitro Culturesmentioning

confidence: 99%

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Genome-wide identification of MATE family, and functional analysis of MATE involved in anthocyanin accumulation in Daucus carota

Saad

Kumar

Puthusseri

et al. 2022

Preprint

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Multidrug and toxic compound extrusion (MATE) is a family of membrane transporters that transport ions and secondary metabolites like anthocyanins in plants. Although various research on MATE has been carried out on different plant species, this is the first comprehensive report to exploit the Daucus carota genome to identify the MATE gene family. We investigated the chromosomal distribution, gene duplication, motif identification, cis-element analysis, in vitro cultures, stress treatment, protein docking, and simulation of DcMATEs. Our studies identified 45 DcMATEs through genome-wide analysis and detected five segmental and six tandem duplications from its genome. The chromosome distribution, phylogenetic analysis, and cis-regulatory elements revealed the structural diversity and numerous functions associated with the DcMATEs. Further, we quantified anthocyanin content and identified the co-expression of DcMATE21 with anthocyanin biosynthesis genes in in vitro cultures under ABA, SA, MJ, SNP, and PHE stress treatments. In addition, membrane dynamics of DcMATE21 with anthocyanin (cyanidin-3-glucoside) identified the binding pocket showing extensive H-bond interactions with ten amino acids present in the transmembrane helix of 7, 8, and 10 of DcMATE21. This investigation speculates the involvement of DcMATE21 in anthocyanin accumulation in in vitro cultures of D. carota.

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“…In cosmetics, active molecules derived from plant cell cultures are used in skincare and hair care products [ 15 ]. Previous reports have described these achievements, including examples of Perilla frutescens , Vitis vinifera , Daucus carota , Ajuga pyramidalis , Vaccinium pahalae , etc., for large-scale anthocyanin production [ 16 , 17 , 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Anthocyanin Production in Tobacco Cells

Carpi,

Rahim,

Marin

et al. 2023

IJMS

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Plant cell cultures have emerged as a promising tool for producing active molecules due to their numerous advantages over traditional agricultural methods. Flavonols, and anthocyanin pigments in particular, together with other phenolic compounds such as chlorogenic acid, are known for their beneficial health properties, mainly due to their antioxidant, antimicrobial, and anti-inflammatory activities. The synthesis of these molecules is finely regulated in plant cells and controlled at the transcriptional level by specific MYB and bHLH transcription factors that coordinate the transcription of structural biosynthetic genes. The co-expression of peach PpMYB10.1 and PpbHLH3 in tobacco was used to develop tobacco cell lines showing high expression of both the peach transgenes and the native flavonol structural genes. These cell lines were further selected for fast growth. High production levels of chlorogenic acid, anthocyanins (mainly cyanidin 3-rutinoside), and other phenolics were also achieved in pre-industrial scale-up trials. A single-column-based purification protocol was developed to produce a lyophile called ANT-CA, which was stable over time, showed beneficial effects on cell viability, and had antioxidant, anti-inflammatory, antibacterial, and wound-healing activities. This lyophile could be a valuable ingredient for food or cosmetic applications.

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Salt Stress-Induced Anthocyanin Biosynthesis Genes and MATE Transporter Involved in Anthocyanin Accumulation in Daucus carota Cell Culture

Cited by 15 publications

References 46 publications

Characterization of Salt-Tolerant Cultivars of Date Palm Based on Morphological and Biochemical Responses Under Salinity Stress

Characterization of Salt-Tolerant Cultivars of Date Palm Based on Morphological and Biochemical Responses Under Salinity Stress

Genome-wide identification of MATE family, and functional analysis of MATE involved in anthocyanin accumulation in Daucus carota

Optimization of Anthocyanin Production in Tobacco Cells

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