In-Taken Labeling and in Vivo Tracing Foodborne Probiotics via DNA-Encapsulated Persistent Luminescence Nanoprobe Assisted Autofluorescence-Free Bioimaging

Abstract

An in vivo probing strategy that can real-time and in situ trace target probiotics inside the living body is herein proposed by employing plasmid-like DNA as in-taken assistance, persistent luminescence nanophosphors (PLNPs) as optical labeling, and background-free fluorescence bioimaging as signal readout. PLNPs with superlong afterglow and excellent biocompatibility and stability were surface-modified by DNA molecules with a specific sequence, which greatly promoted the nanoparticle penetration into the bacteria and facilitated the in vivo bioimaging with high sensitivity and signal-to-noise ratio. Compared with the previous surface-labeling strategy by antibody recognition, the in-taken optical labeling demonstrated improved stability, and reached ideal results of real-time and in situ monitoring the in vivo behaviors of target probiotics, supporting the further development of in vivo investigation methodology for foodborne probiotics. Moreover, such a strategy offers a promising platform that leverage nanoscience to food nutrition as well as food-safety research, aiming to collect more accurate and fresh information from the living body.

Bacterial Biofilm Bioinspired Persistent Luminescence Nanoparticles with Gut-Oriented Drug Delivery for Colorectal Cancer Imaging and Chemotherapy

“…The results showed that LRM coating could induce colonization of nanomaterials in the colorectum, which could significantly prolong the time of drug existence in the colorectum. Moreover, the fluorescence in ZGGO@SiO 2 @LRM group was still detectable after excitement at 24 h. The results were similar to those reported in the literatures. − Because the LRM coating could tolerate the digestion of gastric acid, the oral administration of 5-FU ZGGO@SiO 2 @LRM avoided intravenous administration and further causing damage to immune organs such as liver and spleen. ZGGO@SiO 2 @LRM remained in the colorectum for more than 24 h, which could prolong the release time of chemotherapeutic drugs and increase the concentration of chemotherapeutic drugs in the colorectum.…”

Chromium-Doped Zinc Gallate Near-Infrared Persistent Luminescence Nanoparticles in Autofluorescence-Free Biosensing and Bioimaging: A Review

“…After oral administration, the NP-labeled L. reuteri could be tracked in the gastrointestinal tract in vivo , and the distribution of L. reuteri in vivo could be monitored in real-time (Figure ). Wang et al modified plasmid-like DNA on the surface of ZGC and simultaneously assisted ZGC NPs to enter the probiotics ( L. casei ) through electroporation. Compared with previous strategies of surface labeling by antibody recognition, this method of bacterial encapsulation showed higher stability and achieved ideal results of real-time and highly sensitive biosensing of in vivo behavior of L. casei , which presents a new method for the study of foodborne probiotics in vivo .…”

Recent Advances of Persistent Luminescence Nanoparticles in Bioapplications

“…PL imaging allows noninvasive tracking and imaging for visualizing various biological processes of migrating cells, which might be crucial for the biological fate and progression of some diseases. Up to now, imaging of Raw 264.7 macrophages (a phagocytic cell line), stem cells, breast cancer cells, J774A.1 macrophages have been widely studied by labeling with PLNPs [ 91 – 95 ]. Maldiney et al and Chuang et al reported the noninvasive long-term in vivo tracking of RAW 264.7 cells with PLNPs.…”