In‐syringe vortex‐assisted emulsification microextraction with solidified floating organic drop for the separation of aflatoxins from feed before their determination by HPLC

Abstract

A simple and rapid in‐syringe vortex‐assisted emulsification microextraction with solidified floating organic drop was developed for the extraction of aflatoxins (B1 and B2) from feed before their determined by high‐performance liquid chromatography. Several experimental factors affect the microextraction efficiency such as sample pH, vortex time, type of extraction solvent, and sample volume that have been evaluated and optimized. The calibration curves were linear in the range of 50.0–500.0 and 40.0–400.0 ng kg−1 for aflatoxin B1 and aflatoxin B2, respectively. The limits of detection were found to be 16.0 and 12.0 ng kg−1 for aflatoxin B1 and aflatoxin B2, respectively. The inter‐day and intra‐day precisions of the developed method were in the range of 5.3–9.3%. Finally, the developed microextraction method was successfully used in the extraction and quantification of aflatoxins in several feed samples.