In-straw cryoprotectant dilution of IVP bovine blastocysts vitrified in hand-pulled glass micropipettes

Vieira, Arnaldo Diniz; Forell, Fabiana; Feltrin, Cristiano; Rodrigues, José Luiz

doi:10.1016/j.anireprosci.2006.06.010

Cited by 29 publications

(27 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Therefore, in most studies of embryo cryopreservation, either all blastocysts harvested on Day 7 were used, or a subset was chosen, based on morphological characteristics. In the present study, the cryosurvival rate of Day 7 IVF-derived blastocysts achieved using the Cryotop (86%) was slightly lower than 94% survival reported using the same cryodevice [17], and was comparable to or even higher than those achieved using other cryodevices for MVC vitrification, e.g., open-pulled straws (OPS; 89 to 97%) [33], hand-pulled glass micropipettes (84 to 94%) [34], and electron microscope grids (58 to 98%) [8]. Moreover, in the present study, Day 8 blastocysts had similar survivability to their Day 7 counterparts (derived from either IVF or ICSI).…”

Section: Discussionmentioning

confidence: 85%

Vitrification of ICSI- and IVF-derived bovine blastocysts by minimum volume cooling procedure: effect of developmental stage and age

et al. 2010

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 85%

Vitrification of ICSI- and IVF-derived bovine blastocysts by minimum volume cooling procedure: effect of developmental stage and age

et al. 2010

View full text Add to dashboard Cite

“…To determine the effect of the super‐cooled LN 2 (CoolLN 2 ) on cleavage and blastocyst rates obtained after immature oocyte vitrification, selected COCs were randomly allocated to three groups: (i) non‐vitrified control group, (ii) vitrified into normal LN 2 (−196°C = boiling point) and (iii) vitrified into CoolLN 2 (≤−200°C = boiling off). Open‐pulled glass micropipettes (GMP; Vieira et al. 2007) were used as vitrification containers for immature oocytes and IVP blastocysts.…”

Section: Methodsmentioning

confidence: 99%

“…Blastocysts derived from vitrified immature oocytes from groups (ii) and (iii) were vitrified according to their respective group (LN 2 or CoolLN 2 ), following our established vitrification procedures (Vieira et al. 2007).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Calves Born after Direct Transfer of Vitrified Bovine In Vitro‐produced Blastocysts Derived from Vitrified Immature Oocytes

Vieira

Forell

Feltrin

et al. 2007

Reprod Domestic Animals

View full text Add to dashboard Cite

Vitrification has been the method of choice for the cryopreservation of bovine oocytes, as rapid cooling decreases chilling sensitivity. The aim of this study was to determine the in vitro and in vivo survival and the viability of immature oocytes vitrified using super-cooled liquid nitrogen. Immature oocytes were randomly allocated to three groups: (i) non-vitrified control group, (ii) vitrified in normal (-196 degrees C) liquid nitrogen (LN(2)) and (iii) vitrified in super-cooled LN(2) (< or =-200 degrees C). Open-pulled glass micropipettes were used as vitrification containers. Immature oocytes were in vitro-matured, fertilized and cultured to the blastocyst stage. In vitro viability was assessed by cleavage and blastocyst rates on days 2 and 7 of culture respectively. Vitrified blastocysts derived from the immature vitrified oocytes were directly transferred to synchronous recipients. The in vitro embryo development of vitrified immature oocytes was not influenced by the LN(2) state. After direct transfer (one embryo per recipient) of 16 embryos obtained from immature vitrified oocytes (eight from each vitrified group), two healthy calves were born in each group. These results indicated that vitrification of immature bovine oocytes using glass micropipettes under normal or super-cooled LN(2), resulted in viable blastocysts and live calves following in vitro embryo production.

show abstract

“…Several freezing techniques, such as open-pulled straw (OPS) (Vieira et al, 1999) and cryotops (Inaba et al, 2011), have been successfully utilized in yak breeding. Slow-rate freezing and vitrification are also used to cryopreserve IVP bovine embryos.…”

mentioning

confidence: 99%

Cryosurvival and pregnancy rates: One‐step protocol for freezing–thawing Shangri‐la Yak (Bos grunniens) Embryos

Jia

Yang

Zhang

et al. 2018

Reprod Domestic Animals

View full text Add to dashboard Cite

The yak is one of the most important and economically useful animals for highlanders. The decline in the yak population requires effective measures for the conservation and multiplication of elite germplasm. A standardized protocol will simplify the freezing and warming of yak embryos in straw and facilitate embryo transfer. In this work, we investigated a one-step protocol that uses a stable basal medium, which comprised a warming medium (1.08 M sucrose) and a freezing medium (EFS40). We also assessed the effects of the new transfer method on embryo survival. A total of 145 yak frozen embryos were thawed in a standard medium system. The one-step protocol led to a high recovery percentage (84.93) of yak embryos that survived vitrification and warming. The in vitro survival rates of these embryos significantly different from those of embryos frozen-thawed via the conventional method. The 95 embryos frozen-thawed via our one-step protocol were then implanted in selected recipients. Thirty-six singleton pregnancies were established. In conclusion, the proposed one-step method is a simple, safe, and standardized freezing-thawing protocol that ensures embryo survival and quality under field conditions. This study establishes new possibilities for the widespread use of embryo transfer in yaks.

show abstract

In-straw cryoprotectant dilution of IVP bovine blastocysts vitrified in hand-pulled glass micropipettes

Cited by 29 publications

References 18 publications

Vitrification of ICSI- and IVF-derived bovine blastocysts by minimum volume cooling procedure: effect of developmental stage and age

Vitrification of ICSI- and IVF-derived bovine blastocysts by minimum volume cooling procedure: effect of developmental stage and age

Calves Born after Direct Transfer of Vitrified Bovine In Vitro‐produced Blastocysts Derived from Vitrified Immature Oocytes

Cryosurvival and pregnancy rates: One‐step protocol for freezing–thawing Shangri‐la Yak (Bos grunniens) Embryos

Contact Info

Product

Resources

About