In Situ Thermal Denaturation of Proteins in Dunning AT-1 Prostate Cancer Cells: Implication for Hyperthermic Cell Injury

He, Xiaoming; Wolkers, Willem F.; Crowe, John H.; Swanlund, David J.; Bischof, John C.

doi:10.1114/b:abme.0000042226.97347.de

Cited by 79 publications

(108 citation statements)

References 46 publications

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“…Although cells attached to cell-culture-treated substrate has been dominantly used in studies on thermally induced cell injury, cells suspended in media have also been used to investigate the protein and lipid change in intact cells. 6,24,31,32,46 Therefore, it is interesting and important to compare the thermal injury behavior between suspended and attached cells. Some studies reported that suspended bacterial cells are more susceptible to heat than attached ones, 12,16 such study on mammalian (tumor) cell lines has not been reported to our knowledge.…”

Section: Introductionmentioning

confidence: 99%

The Kinetics of Thermal Injury in Human Renal Carcinoma Cells

Bischof

2005

Ann Biomed Eng

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In this study, the thermal injury behavior of both suspended and attached SN12 human renal carcinoma cells (RCC) under thermal therapy conditions (i.e., heating cells to elevated temperature for seconds to minutes) was investigated using a non-isothermal method. This non-isothermal method entailed heating the cells using a programmable heating stage from room temperature at 130 degrees C min(-1) to various peak temperatures from 45 to 70 degrees C, held for 0-10 min, and then cooling down to room temperature at 65 degrees C min(-1). It was found that the suspended SN12 cells are more heat susceptible than attached ones. The non-isothermal portions (i.e., the heat-up and cool-down portions) of the thermal histories were found to be able to cause significant injury (> 10%) in both suspended and attached SN12 cells when the peak temperature is above 60 degrees C. Therefore, a non-isothermal method, which accounts for both the isothermal and non-isothermal portions of the thermal histories, was used to extract the kinetic parameters (i.e., the activation energy and frequency factor) in the Arrhenius injury model for SN12 cells. Furthermore, these results suggest that this non-isothermal method can be used to extract kinetic parameters from in vivo heating studies using minimally invasive surgical probes, where it is very difficult to get a thermal history in tissue with a dominant isothermal portion.

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Section: Introductionmentioning

confidence: 99%

The Kinetics of Thermal Injury in Human Renal Carcinoma Cells

Bischof

2005

Ann Biomed Eng

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“…5,9,10 The irreversible denaturation of proteins is assumed to be the ratelimiting step in heating-induced tissue coagulation and also has been shown to be directly correlated with cell death. [11][12][13] Tissue damage can be modeled as a change in state based on an Arrhenius rate process.…”

Section: Introductionmentioning

confidence: 99%

Quantitative comparison of thermal dose models in normal canine brain

et al. 2010

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Purpose: Minimally invasive thermal ablative therapies as alternatives to conventional surgical management of solid tumors and other pathologies is increasing owing to the potential benefits of performing these procedures in an outpatient setting with reduced complications and comorbidity. Magnetic resonance temperature imaging ͑MRTI͒ measurement allows existing thermal dose models to use the spatiotemporal temperature history to estimate the thermal damage to tissue. However, the various thermal dose models presented in the literature employ different parameters and thresholds, affecting the reliability of thermal dosimetry. In this study, the authors quantitatively compared three thermal dose models ͑Arrhenius rate process, CEM 43 , and threshold temperature͒ using the dice similarity coefficient ͑DSC͒. Methods: The DSC was used to compare the spatial overlap between the region of thermal damage as predicted by the models for in vivo normal canine brain during thermal therapy to the region of thermal damage as revealed by contrast-enhanced T1-weighted images acquired immediately after therapy ͑Ͻ20 min͒. The outer edge of the hyperintense rim of the ablation region was used as the surrogate marker for the limits of thermal coagulation. The DSC was also used to investigate the impact of varying the thresholds on each models' ability to predict the zone of thermal necrosis. Results: At previously reported thresholds, the authors found that all three models showed good agreement ͑defined as DSCϾ 0.7͒ with post-treatment imaging. All three models examined across the range of commonly applied thresholds consistently showed highly accurate spatial overlap, low variability, and little dependence on temperature uncertainty. DSC values corresponding to cited thresholds were not significantly different from peak DSC values. Conclusions: Thus, the authors conclude that the all three thermal dose models can be used as a reliable surrogate for postcontrast tissue damage verification imaging in rapid ablation procedures and can also be used to enhance the capability of MRTI to control thermal therapy in real time.

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“…The fitting was done with a non-linear optimization procedure (the flexible tolerance method 28 ) to minimize the root mean square between the model predictions and the experimental data, which has been used by the authors in a number of studies to extract multiple parameters in a variety of models for various applications. 22,23,25,26 …”

Section: Determination Of Model Parametersmentioning

confidence: 98%

Biotransport Phenomena in Freezing Mammalian Oocytes

Yang

Veres²,

Szalai³

et al. 2010

Ann Biomed Eng

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Water transport across the cell plasma membrane and intracellular ice formation (IIF)-the two biophysical events that may cause cell injury during cryopreservation-were studied by cryomicroscopy and modeling using mammalian (Peromyscus) oocytes. Unusually high activation energy for water transport across the cell plasma membrane was identified indicating that the water transport process is unusually sensitive to temperature (and cooling rate). Although literally all studies on IIF were conducted using protocols with ice-seeding (seeding extracellular ice usually at ≥-7 °C), it is not used for cell cryopreservation by vitrification that is becoming increasingly popular today. In this article, we show that ice-seeding has a significant impact on IIF. With ice-seeding and cooling at 60 °C/min, IIF was observed to occur over a wide range from approximately -8 to -48 °C with a clear change of the ice nucleation mechanism (from surface- to volume-catalyzed nucleation) at approximately -43 °C. On the contrary, without ice-seeding, IIF occurred over a much narrower range from approximately -19 to -27 °C without a noticeable change of the nucleation mechanism. Moreover, the kinetics of IIF without ice-seeding was found to be strongly temperature (and cooling rate) dependent. These findings indicate the importance of quantifying the IIF kinetics in the absence of ice-seeding during cooling for development of optimal vitrification protocols of cell cryopreservation.

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In Situ Thermal Denaturation of Proteins in Dunning AT-1 Prostate Cancer Cells: Implication for Hyperthermic Cell Injury

Cited by 79 publications

References 46 publications

The Kinetics of Thermal Injury in Human Renal Carcinoma Cells

The Kinetics of Thermal Injury in Human Renal Carcinoma Cells

Quantitative comparison of thermal dose models in normal canine brain

Biotransport Phenomena in Freezing Mammalian Oocytes

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