In Situ Scatheless Cell Detachment Reveals Correlation between Adhesion Strength and Viability at Single‐Cell Resolution

Abstract

Single-cell biology provides insights into some of the most fundamental processes in biology and promotes the understanding of life's mysteries. As the technologies to study single-cells expand, they will require sophisticated analytical tools to make sense of various behaviors and components of single-cells as well as their relations in the adherent tissue culture. In this paper, we revealed cell heterogeneity and uncovered the connections between cell adhesion strength and cell viability at single-cell resolution by extracting single adherent cells of interest from a standard tissue culture by using a microfluidic chip-based live single-cell extractor (LSCE). We believe that this method will provide a valuable new tool for single-cell biology.

Microfluidics‐Based Biomaterials and Biodevices

“…F) A microfluidics‐based live single‐cell extraction system. Reproduced with permission . Copyright 2018, Wiley‐VCH.…”

“…In this system, single cells and assay reagents are compartmentalized in droplets and kinetically analyzed in two dimensional (2D) droplet arrays. Recently, a microfluidics‐based live single‐cell extractor is used to explore cell heterogeneity and connections (Figure F) . By adjusting the flow ratio between injection and aspiration channel, a stable microjet with appropriate size for cell extraction is generated in microfluidic chip.…”

Microfluidic Devices in the Fast‐Growing Domain of Single‐Cell Analysis

“…And (f) Aspiration‐based module for single‐cell isolation from cell culture on petri‐dish, reprinted with permission from Ref. . Copyright 2017 Wiley‐VCH.…”

“…It is one of the most important characteristics related to cell spreading area, cell size, cell activity, and intercellular metabolites. Recently, Mao et al . reported a live single‐cell extractor (LSCE) to extract a single‐cell adhered on a cell‐culture dish for studying the cell heterogeneity (Figure f).…”

“…[106,122] The strengtho fc ellsa dhesion can be crucial for the assembly of individual cells of identicala nd different types into 3-dimensional tissues in the human body.I ti so ne of the most important characteristics related to cell spreading area,c ell size, cell activity,a nd intercellularm etabolites. Recently,M ao et al [123] reported al ive single-cell extractor( LSCE) to extract a single-cell adhered on ac ell-culture dish for studying the cell heterogeneity (Figure 6f). The tip of the LSCE extractor was immersed perpendicular to the petri-dish containing the cells for extraction.…”