In situ ruminal phosphorus degradation of selected three classes of feedstuffs in goats

Wang, Min; Hu, Yifeng; Tan, Zhiliang; Tang, Shaoxun; Sun, Zhihong; Han, Xuefeng

doi:10.1016/j.livsci.2007.12.016

Cited by 8 publications

(5 citation statements)

References 15 publications

(14 reference statements)

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“…The goats, with the body weight of 25 ± 2.0 kg, were penned individually in stainless metabolic cages, and had free access to fresh water throughout the entire experiment. Diets containing approximately 70% roughage and 30% concentrate were formulated (DM basis) and offered twice in equal amounts at 08.00 and 20.00 h to supply 1.3 times maintenance requirement of metabolizable energy according to our previous studies [ 13 ]. Before the morning feeding, rumen fluid was collected from each goat using a manual vacuum and immediately transferred into pre-warmed (39°C) thermos flasks (2 L) that was filled with CO 2 .…”

Section: Methodsmentioning

confidence: 99%

Influence of Oleic Acid on Rumen Fermentation and Fatty Acid Formation In Vitro

Tang

et al. 2016

PLoS ONE

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A series of batch cultures were conducted to investigate the effects of oleic acid (OA) on in vitro ruminal dry matter degradability (IVDMD), gas production, methane (CH4) and hydrogen (H2) production, and proportion of fatty acids. Rumen fluid was collected from fistulated goats, diluted with incubation buffer, and then incubated with 500 mg Leymus chinensis meal supplemented with different amounts of OA (0, 20, 40, and 60 mg for the CON, OA20, OA40 and OA60 groups, respectively). Incubation was carried out anaerobically at 39°C for 48 h, and the samples were taken at 12, 24 and 48 h and subjected to laboratory analysis. Supplementation of OA decreased IVDMD, the cumulative gas production, theoretical maximum of gas production and CH4 production, but increased H2 production. However, no effect was observed on any parameters of rumen fermentation (pH, ammonia, production of acetate, propionate and butyrate and total volatile fatty acid production). The concentrations of some beneficial fatty acids, such as cis monounsaturated fatty acids and conjugated linoleic acid (CLA) were higher (P < 0.05) from OA groups than those from the control group at 12 h incubation. In summary, these results suggest that the OA supplementation in diet can reduce methane production and increase the amount of some beneficial fatty acids in vitro.

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Section: Methodsmentioning

confidence: 99%

Influence of Oleic Acid on Rumen Fermentation and Fatty Acid Formation In Vitro

Tang

et al. 2016

PLoS ONE

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“…These animals were maintained individually in metabolic cages under controlled condition with fresh water available ad libitum. A diet consisting of forage and concentrate was offered twice daily in equal amounts at 8:00 a.m. and 6:00 p.m. to each goat to supply 1.3× maintenance requirement of metabolizable energy according to our previous studies . After 15 days of feeding, the goats were slaughtered for sample collection.…”

Section: Materials and Methodsmentioning

confidence: 99%

Cloning, Phylogenetic Analysis, and Distribution of Free Fatty Acid Receptor GPR120 Expression along the Gastrointestinal Tract of Housing versus Grazing Kid Goats

Tao

Liu

et al. 2016

J. Agric. Food Chem.

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G-protein-coupled receptor 120 (GPR120) is reported as a long-chain fatty acid (LCFA) receptor that elicits free fatty acid (FFA) regulation on metabolism homeostasis. The study aimed to clone the gpr120 gene of goats (g-GPR120) and subsequently investigate phylogenetic analysis and tissue distribution throughout the digestive tracts of kid goats, as well as the effect of housing versus grazing (H vs G) feeding systems on GPR120 expression. Partial coding sequence (CDS) of g-GPR120 was cloned and submitted to NCBI (accession no. KU161270 ). Phylogenetic analysis revealed that g-GPR120 shared higher homology in both mRNA and amino acid sequences for ruminants than nonruminants. Immunochemistry, real-time PCR, and Western blot analysis showed that g-GPR120 was expressed throughout the digestive tracts of goats. The expression of g-GPR120 was affected by feeding system and age, with greater expression of g-GPR120 in the G group. It was concluded that the g-GPR120-mediated LCFA chemosensing mechanism is widely present in the tongue and gastrointestinal tract of goats and that its expression can be affected by feeding system and age.

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“…In situ rumen degradation of DM, CP, and NDF of the SBP and four experimental diets was determined using the method described by Wang et al (2008) with three ruminally cannulated adult 1/2 Dorper × 1/2 small thin-tailed wethers. The basal diet (% of DM) consisted of 40.3% concentrate mixture, 35.5% corn straw, and 24.2% alfalfa hay offered two times daily ad libitum.…”

Section: In Situ Degradationmentioning

confidence: 99%

Nutrient digestibility, rumen microbial protein synthesis, and growth performance in sheep consuming rations containing sea buckthorn pomace1

Hao

Diao

et al. 2018

Journal of Animal Science

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This experiment was conducted to investigate nutrient digestibility, rumen microbial protein synthesis, and growth performance when different proportions of sea buckthorn pomace (SBP) were included in the diet of sheep. A total of forty1/2 Dorper × 1/2 thin-tailed Han ram lambs (BW = 22.2 ± 0.92 kg, age =120 ± 11 d; mean ± SD) were selected and divided into four groups in a randomized design and were randomly allocated to one of four treatment diets. Diets were formulated isonitrogenously and contained different levels of SBP: 1) 0% SBP (control), 2) 7.8% of DM SBP (8SBP), 3) 16.0% of DM SBP (16SBP), and 4) 23.5% of DM SBP (24SBP). A portion of corn and forages were replaced with SBP. DMI and ADG increased linearly (P = 0.001), but feed efficiency was not affected (P ≥ 0.460) by increasing SBP inclusion rate. As the SBP inclusion increased, OM, NDF, and ADF digestibility decreased linearly (P ≤ 0.005) and that CP increased linearly (P = 0.012). Response to inclusion level of SBP was quadratic (P = 0.003) for the estimated microbial CP yield with the greatest at intermediate SBP levels. For intestinally absorbable dietary protein, quadratic (P = 0.029) effects were observed among treatments. The metabolizable protein (MP) supplies were linearly (P < 0.0001) improved with increasing SBP inclusion rate. The results indicated that SBP can be incorporated in the ration of ram lambs and improve MP supply and ADG. However, high content of it in the diet was adverse for nutrient digestibility. The optimal proportion was 16.0% under the condition of this experiment.

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In situ ruminal phosphorus degradation of selected three classes of feedstuffs in goats

Cited by 8 publications

References 15 publications

Influence of Oleic Acid on Rumen Fermentation and Fatty Acid Formation In Vitro

Influence of Oleic Acid on Rumen Fermentation and Fatty Acid Formation In Vitro

Cloning, Phylogenetic Analysis, and Distribution of Free Fatty Acid Receptor GPR120 Expression along the Gastrointestinal Tract of Housing versus Grazing Kid Goats

Nutrient digestibility, rumen microbial protein synthesis, and growth performance in sheep consuming rations containing sea buckthorn pomace1

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