In situ reactive extraction of itaconic acid during fermentation of Aspergillus terreus

Kreyenschulte, Dirk; Heyman, Benedikt; Eggert, Armin; Maßmann, Tim; Kalvelage, Christina; Kossack, Ronja; Regestein, Lars; Jupke, Andreas; Büchs, Jochen

doi:10.1016/j.bej.2018.04.014

Cited by 43 publications

(34 citation statements)

References 54 publications

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“…The pH increased during the galacturonic acid consumption phase to 7.1. This behavior is typical during the consumption of acidic carbon sources as they cross the cell membrane only in their protonated form [56]. At the end of the cultivation, 1.45 g/L ammonium chloride remained in the culture supernatant, indicating that the elevated concentration of 4 g/L ammonium chloride was sufficient to prevent nitrogen limitation.…”

Section: Resultsmentioning

confidence: 99%

Online evaluation of the metabolic activity of Ustilago maydis on (poly)galacturonic acid

et al. 2018

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BackgroundPectin is a rather complex and highly branched polysaccharide strengthening the plant cell wall. Thus, many different pectinases are required for an efficient microbial conversion of biomass waste streams with a high pectin content like citrus peel, apple pomace or sugar beet pulp. The screening and optimization of strains growing on pectic substrates requires both, quantification of the residual substrate and an accurate determination of the enzymatic activity. Galacturonic acid, the main sugar unit of pectin, is an uncommon substrate for microbial fermentations. Thus, growth and enzyme production of the applied strain has to be characterized in detail to understand the microbial system. An essential step to reach this goal is the development of online monitoring tools.ResultsIn this study, a method for the online determination of residual substrate was developed for the growth of the plant pathogenic fungus Ustilago maydis on pectic substrates such as galacturonic acid. To this end, an U. maydis strain was used that expressed a heterologous exo-polygalacturonase for growth on polygalacturonic acid. The growth behavior on galacturonic acid was analyzed by online measurement of the respiration activity. A method for the online prediction of the residual galacturonic acid concentration during the cultivation, based on the overall oxygen consumption, was developed and verified by offline sampling. This sensitive method was extended towards polygalacturonic acid, which is challenging to quantify via offline measurements. Finally, the enzymatic activity in the culture supernatant was calculated and the enzyme stability during the course of the cultivation was confirmed.ConclusionThe introduced method can reliably predict the residual (poly)galacturonic acid concentration based on the overall oxygen consumption. Based on this method, the enzymatic activity of the culture broth of an U. maydis strain expressing a heterologous exo-polygalacturonase could be calculated. It was demonstrated that the method is especially advantageous for determination of low enzymatic activities. In future, it will be applied to U. maydis strains in which the number of produced hydrolytic enzymes is increased for more efficient degradation.Electronic supplementary materialThe online version of this article (10.1186/s13036-018-0128-1) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Online evaluation of the metabolic activity of Ustilago maydis on (poly)galacturonic acid

et al. 2018

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“…Moreover, being similar to the chemicals produced from glucose by chemical catalysis, xylose can be converted into various important precursors like polyols such as 1,3‐propanediol, 2,3‐butanediol, and organic diacids such as succinic acid, itaconic acid, fumaric acid, muconic acid (further to adipic acid), and other functional acids such as lactic acid, and 3‐hydroxypropionic acid (3‐HPA) (Fig. ).…”

Section: Nanocellulosementioning

confidence: 99%

High value‐added monomer chemicals and functional bio‐based materials derived from polymeric components of lignocellulose by organosolv fractionation

Zhang

Cai

Qin

et al. 2019

Biofuels Bioprod Bioref

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Organosolv fractionation (OF) is a promising method for lignocellulosic biomass biorefining to produce biofuels, biochemicals, and biomaterials. The fractionated polymeric components may be good feedstocks to produce bio‐based materials such as green polymers via chemical or biological conversion. However, related work to specify the bio‐based materials production via organosolv fractionation of lignocellulosic biomass is relatively scarce. In this work we have provided a comprehensive review of typical organosolv fractionation for isolating cellulose, hemicelluloses, and lignin, as well as the high value‐added monomer chemicals and functional materials derived from the fractionated components and their potential applications developed in recent years, primarily including the modified cellulose fibers for polymer materials, cellulose nanocrystals (CNCs), polysaccharide‐derived platform precursors for synthesis of bio‐based polymers, lignin‐derived dispersants, surfactants, carbon materials, and hemicellulose‐derived functional materials, etc. This work suggests that organosolv fractionation of lignocellulosic biomass could be a good entry to biomass refinery for high value‐added and functional materials production. However, there are still many challenges that should be overcome in terms of the fundamental, technical, and economic aspects for the organosolv fractionation process, formation of precursor compounds, synthesis of the bio‐based materials, design of product properties and functions, and the integration of the entire process. © 2019 Society of Chemical Industry and John Wiley & Sons, Ltd

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“…b Batch fermentation of A. terreus with in situ itaconic acid extraction and initial glucose concentrations of 215 g L −1 and 268 g L −1 . Further details were published by Kreyenschulte et al [ 82 ]. c Batch fermentation of U. maydis with additional glucose pulse at 48 h. Further details were published by Geiser et al [ 69 ].…”

Section: Introductionmentioning

confidence: 99%

“…0.41 g IA g initial glucose −1 . Therefore, in situ extraction of itaconic acid is a powerful tool to increase the efficiency of the process [ 82 , 83 ]. Further details are described in Kreyenschulte et al [ 82 ].…”

Section: Introductionmentioning

confidence: 99%

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From beech wood to itaconic acid: case study on biorefinery process integration

Regestein

Klement

Grande

et al. 2018

Biotechnol Biofuels

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Renewable raw materials in sustainable biorefinery processes pose new challenges to the manufacturing routes of platform chemicals. Beside the investigations of individual unit operations, the research on process chains, leading from plant biomass to the final products like lactic acid, succinic acid, and itaconic acid is increasing. This article presents a complete process chain from wooden biomass to the platform chemical itaconic acid. The process starts with the mechanical pretreatment of beech wood, which subsequently is subjected to chemo-catalytic biomass fractionation (OrganoCat) into three phases, which comprise cellulose pulp, aqueous hydrolyzed hemicellulose, and organic lignin solutions. Lignin is transferred to further chemical valorization. The aqueous phase containing oxalic acid as well as hemi-cellulosic sugars is treated by nanofiltration to recycle the acid catalyst back to the chemo-catalytic pretreatment and to concentrate the sugar hydrolysate. In a parallel step, the cellulose pulp is enzymatically hydrolyzed to yield glucose, which—together with the pentose-rich stream—can be used as a carbon source in the fermentation. The fermentation of the sugar fraction into itaconic acid can either be performed with the established fungi Aspergillus terreus or with Ustilago maydis. Both fermentation concepts were realized and evaluated. For purification, (in situ) filtration, (in situ) extraction, and crystallization were investigated. The presented comprehensive examination and discussion of the itaconate synthesis process—as a case study—demonstrates the impact of realistic process conditions on product yield, choice of whole cell catalyst, chemocatalysts and organic solvent system, operation mode, and, finally, the selection of a downstream concept.

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In situ reactive extraction of itaconic acid during fermentation of Aspergillus terreus

Cited by 43 publications

References 54 publications

Online evaluation of the metabolic activity of Ustilago maydis on (poly)galacturonic acid

Online evaluation of the metabolic activity of Ustilago maydis on (poly)galacturonic acid

High value‐added monomer chemicals and functional bio‐based materials derived from polymeric components of lignocellulose by organosolv fractionation

From beech wood to itaconic acid: case study on biorefinery process integration

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