In Situ Quantification of Polyhydroxybutyrate in Photobioreactor Cultivations of Synechocystis sp. Using an Ultrasound-Enhanced ATR-FTIR Spectroscopy Probe

Doppler, Philipp; Gasser, Christoph; Kriechbaum, Ricarda; Ferizi, Ardita; Spadiut, Oliver

doi:10.3390/bioengineering8090129

Cited by 5 publications

(3 citation statements)

References 41 publications

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“…A strong band at 1719 cm −1 corresponds to the C=O stretching vibration of the PHB, while the vibrational bands related to the ester C-O-C bond appear at 1275, 1181, and 1100 cm −1 [31]. The FTIR peaks occurring in the 2800-3050 cm −1 range are due to CH 2 and CH 3 stretching vibrations of PHB and DBUA plasticizer [32,33]. The absorption band at 1130 cm −1 may be assigned to the CH 3 rocking vibrations [32].…”

Section: Resultsmentioning

confidence: 99%

Development of Polyhydroxybutyrate-Based Packaging Films and Methods to Their Ultrasonic Welding

Talaniuk,

Godzierz,

Vashchuk

et al. 2023

Materials

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This study developed a technical task associated with the formation of welded joints based on biodegradable polymers and their subsequent physicochemical characterization. The primary objective was to establish the effect of the welding process and modification of natural poly(3-hydroxybutyrate) (PHB) with N,N-dibutylundecenoylamide (DBUA) as a plasticizing agent on the structure and properties of PHB-based biopolymer materials as well as the process and structure of welded joints formation using ultrasonic welding technique. The weldability of biodegradable layers based on PHB and PHB/DBUA mixture was ultrasonically welded and optimized using a standard Branson press-type installation. The effect of the DBUA plasticizer and welding process on the structure of PHB-based biodegradable material was investigated using scanning electron microscopy, X-ray diffraction, FT-IR spectroscopy, differential scanning calorimetry, and thermomechanical analysis. The results confirmed that the DBUA acted as an effective plasticizer of PHB, contributing to lower crystallinity of the PHB/DBUA mixture (63%) in relation to the crystallinity degree of pure PHB film (69%). Ultrasonic welding resulted in an additional increase (approximately 8.5%) in the degree of crystallinity in the PHB/DBUA in relation to the initial PHB/DBUA mixture. The significant shift toward lower temperatures of the crystallization and melting peaks of PHB modified with DBUA were observed using DSC concerning pure PHB. The melt crystallization process of PHB was affected by welding treatment, and a shift toward higher temperature was observed compared with the unwelded PHB/DBUA sample. The butt-welded joints of biodegradable PHB/DBUA materials made using the ultrasonic method tested for tensile strength have damaged the area immediately outside the joining surface.

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Section: Resultsmentioning

confidence: 99%

Development of Polyhydroxybutyrate-Based Packaging Films and Methods to Their Ultrasonic Welding

Talaniuk,

Godzierz,

Vashchuk

et al. 2023

Materials

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“…The supernatant was analyzed on an UltiMate 3000 HPLC system with diode array detector (DAD) (Thermo Fisher Scientific, Waltham, MA, USA) by an Aminex HPX-87 H column (Bio-Rad Laboratories, Hercules, CA, USA). The method was recently described in detail [ 54 ]. Calibration standards of all investigated C-sources (glucose, fructose, ribose, xylose, acetate and glycerol) were freshly prepared of pure substances (Sigma-Aldrich, St. Louis, MO, USA), diluted and analyzed accordingly.…”

Section: Methodsmentioning

confidence: 99%

Coelastrella terrestris for Adonixanthin Production: Physiological Characterization and Evaluation of Secondary Carotenoid Productivity

et al. 2022

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A novel strain of Coelastrella terrestris (Chlorophyta) was collected from red mucilage in a glacier foreland in Iceland. Its morphology showed characteristic single, ellipsoidal cells with apical wart-like wall thickenings. Physiological characterization revealed the presence of the rare keto-carotenoid adonixanthin, as well as high levels of unsaturated fatty acids of up to 85%. Initial screening experiments with different carbon sources for accelerated mixotrophic biomass growth were done. Consequently, a scale up to 1.25 L stirred photobioreactor cultivations yielded a maximum of 1.96 mg·L−1 adonixanthin in free and esterified forms. It could be shown that supplementing acetate to the medium increased the volumetric productivity after entering the nitrogen limitation phase compared to autotrophic control cultures. This study describes a promising way of biotechnological adonixanthin production using Coelastrella terrestris.

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“…In details, spectroscopic attenuated total reflection Fourier-transformed mid-infrared (ATR-FTIR) spectra for P(3HB) and glycogen, two characteristic storage compounds in the selected model cyanobacterium Synechocystis sp. PCC 6714, were enhanced by ultrasonic particle manipulation, and used for in situ quantification of these compounds throughout the process [ 17 ].…”

Section: Individual Contributionsmentioning

confidence: 99%

Advances in Polyhydroxyalkanoate (PHA) Production, Volume 3

Koller

2022

Bioengineering

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Steadily increasing R&D activities in the field of microbial polyhydroxyalkanoate (PHA) biopolyesters are committed to growing global threats from climate change, aggravating plastic pollution, and the shortage of fossil resources. These prevailing issues paved the way to launch the third Special Issue of Bioengineering dedicated to future-oriented biomaterials, characterized by their versatile plastic-like properties. Fifteen individual contributions to the Special Issue, written by renowned groups of researchers from all over the world, perfectly mirror the current research directions in the PHA sector: inexpensive feedstock like carbon-rich waste from agriculture, mitigation of CO2 for PHA biosynthesis by cyanobacteria or wild type and engineered “knallgas” bacteria, powerful extremophilic PHA production strains, novel tools for rapid in situ determination of PHA in photobioreactors, modelling of the dynamics of PHA production by mixed microbial cultures from inexpensive raw materials, enhanced bioreactor design for high-throughput PHA production by sophisticated cell retention systems, sustainable and efficient PHA recovery from biomass assisted by supercritical water, enhanced processing of PHA by application of novel antioxidant additives, and the development of compatible biopolymer blends. Moreover, elastomeric medium chain length PHA (mcl-PHA) are covered in-depth, inter alia, by introduction of a novel class of bioactive mcl-PHA-based networks, in addition to the first presentation of the new rubber-like polythioester poly(3-mercapto-2-methylpropionate). Finally, the present Special Issue is concluded by a critical essay on past, ongoing, and announced global endeavors for PHA commercialization.

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In Situ Quantification of Polyhydroxybutyrate in Photobioreactor Cultivations of Synechocystis sp. Using an Ultrasound-Enhanced ATR-FTIR Spectroscopy Probe

Cited by 5 publications

References 41 publications

Development of Polyhydroxybutyrate-Based Packaging Films and Methods to Their Ultrasonic Welding

Development of Polyhydroxybutyrate-Based Packaging Films and Methods to Their Ultrasonic Welding

Coelastrella terrestris for Adonixanthin Production: Physiological Characterization and Evaluation of Secondary Carotenoid Productivity

Advances in Polyhydroxyalkanoate (PHA) Production, Volume 3

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