Advances in Polyhydroxyalkanoate (PHA) Production, Volume 3

Koller, Martin

doi:10.3390/bioengineering9070328

Cited by 6 publications

(2 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Along with diverse material properties, PHAs find versatile applications in packaging, biomedicine, and non‐woven fabrics (Mahato et al., 2023). However, despite emulating the features of the seven top‐selling fossil plastics accounting for an annual production of 230 million tonnes, the installed PHA manufacturing capacity in 2021 was estimated at only 48 kt/annum (Mukherjee & Koller, 2023; Ravenstijn, 2022).…”

Section: Introductionmentioning

confidence: 99%

High‐cell‐density fed‐batch strategy to manufacture tailor‐made P(HB‐co‐HHx) by engineered Ralstonia eutropha at laboratory scale and pilot scale

Thiele,

Santolin,

Detels

et al. 2024

Microbial Biotechnology

View full text Add to dashboard Cite

The transition towards a sustainable bioeconomy requires the development of highly efficient bioprocesses that enable the production of bulk materials at a competitive price. This is particularly crucial for driving the commercialization of polyhydroxyalkanoates (PHAs) as biobased and biodegradable plastic substitutes. Among these, the copolymer poly(hydroxybutyrate‐co‐hydroxyhexanoate) (P(HB‐co‐HHx)) shows excellent material properties that can be tuned by regulating its monomer composition. In this study, we developed a high‐cell‐density fed‐batch strategy using mixtures of fructose and canola oil to modulate the molar composition of P(HB‐co‐HHx) produced by Ralstonia eutropha Re2058/pCB113 at 1‐L laboratory scale up to 150‐L pilot scale. With cell densities >100 g L−1 containing 70–80 wt% of PHA with tunable HHx contents in the range of 9.0–14.6 mol% and productivities of up to 1.5 g L−1 h−1, we demonstrate the tailor‐made production of P(HB‐co‐HHx) at an industrially relevant scale. Ultimately, this strategy enables the production of PHA bioplastics with defined material properties on the kilogram scale, which is often required for testing and adapting manufacturing processes to target diverse applications.

show abstract

Section: Introductionmentioning

confidence: 99%

High‐cell‐density fed‐batch strategy to manufacture tailor‐made P(HB‐co‐HHx) by engineered Ralstonia eutropha at laboratory scale and pilot scale

Thiele,

Santolin,

Detels

et al. 2024

Microbial Biotechnology

View full text Add to dashboard Cite

show abstract

“…Their secondary metabolites are rich sources of vitamins, enzymes and toxins which are crucial in many biotechnological industries [7], for instance, in the production of bioplastics from cyanobacteria's polyhydroxy-alkanoates (PHA) [12,13]. PHA accumulates intracellularly in many cyanobacteria species, which can be utilized in bioplastics production with properties such as polyethylene and polypropylene [12]. A wide variety cyanotoxins have been generated from cyanobacteria [14].…”

Section: Introductionmentioning

confidence: 99%

Versatile Applications of Cyanobacteria in Biotechnology

et al. 2022

View full text Add to dashboard Cite

Cyanobacteria are blue-green Gram-negative and photosynthetic bacteria which are seen as one of the most morphologically numerous groups of prokaryotes. Because of their ability to fix gaseous nitrogen and carbon dioxide to organic materials, they are known to play important roles in the universal nutrient cycle. Cyanobacteria has emerged as one of the promising resources to combat the issues of global warming, disease outbreaks, nutrition insecurity, energy crises as well as persistent daily human population increases. Cyanobacteria possess significant levels of macro and micronutrient substances which facilitate the versatile popularity to be utilized as human food and protein supplements in many countries such as Asia. Cyanobacteria has been employed as a complementary dietary constituent of feed for poultry and as vitamin and protein supplement in aquatic lives. They are effectively used to deal with numerous tasks in various fields of biotechnology, such as agricultural (including aquaculture), industrial (food and dairy products), environmental (pollution control), biofuel (bioenergy) and pharmaceutical biotechnology (such as antimicrobial, anti-inflammatory, immunosuppressant, anticoagulant and antitumor); recently, the growing interest of applying them as biocatalysts has been observed as well. Cyanobacteria are known to generate a numerous variety of bioactive compounds. However, the versatile potential applications of cyanobacteria in biotechnology could be their significant growth rate and survival in severe environmental conditions due to their distinct and unique metabolic pathways as well as active defensive mechanisms. In this review, we elaborated on the versatile cyanobacteria applications in different areas of biotechnology. We also emphasized the factors that could impede the implementation to cyanobacteria applications in biotechnology and the execution of strategies to enhance their effective applications.

show abstract