In-situ protein adsorption study on biofunctionalized surfaces using spectroscopic ellipsometry

Goyal, Dileep; Subramanian, Anuradha

doi:10.1016/j.tsf.2009.10.110

Cited by 25 publications

(14 citation statements)

References 36 publications

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“…Spectroscopic Ellipsometry Ellipsometry and UV-vis SE are often used to investigate adsorption dynamics at surfaces and thickness of thin and ultrathin biological films [22][23][24][25][26] and other complex molecular systems [7,8,[27][28][29][30][31].…”

Section: Methodsmentioning

confidence: 99%

Yeast Cytochrome c Monolayer on Flat and Nanostructured Gold Films Studied by UV–Vis Spectroscopic Ellipsometry

et al. 2011

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Spectroscopic ellipsometry (SE) has been used to investigate the adsorption of yeast cytochrome c (YCC) on gold films. YCC is a prototypical molecular system to evaluate the sensitivity and reliability of optical methods in probing conformational changes upon adsorption at surfaces. Measurements have been performed in situ in aqueous solutions at room temperature. We have applied to monolayer-thick YCC films the same scheme of analysis of SE spectra used in our recent works on thiolate self-assembled monolayers on gold, exploiting the so-called δ and δ difference spectra. A comparison between δ and δ spectra obtained from YCC adsorption on flat and cluster-assembled nanostructured gold films, obtained by supersonic cluster beam deposition is proposed. For both kinds of substrate δ and δ , difference spectra show well-defined features related to molecular optical absorptions typical of the so-called heme group, in particular the Soret band at about 410 nm. These features appear in the same position found for molecules in solution, suggesting that YCC native conformation is maintained upon adsorption also in the case of a substrate with typical roughness at the nanoscale.

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Section: Methodsmentioning

confidence: 99%

Yeast Cytochrome c Monolayer on Flat and Nanostructured Gold Films Studied by UV–Vis Spectroscopic Ellipsometry

et al. 2011

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“…Contact angles were determined at three different locations per sample. These were averaged and the OWRK (Owens, Wendt, Rabel and Kaelbe) method was then used to calculate the surface energy of the deposited coatings [21,22]. The water contact angle and surface energy of the deposited coatings were determined on five different sample substrates for each coating process condition.…”

Section: Coating Characterisationmentioning

confidence: 99%

“…There are many methods of measuring this adsorption, such as surface plasmon resonance (SPR), quartz crystal microbalance (QCM) and radiolabelling techniques. Spectroscopic ellipsometry allows real time in situ monitoring of the process of protein adsorption under flow conditions and has been used to study the thickness, adsorption and desorption kinetics of serum proteins on a range of surface chemistries [14,21,22].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Protein Adsorption on Atmospheric Plasma Deposited Coatings Exhibiting Superhydrophilic to Superhydrophobic Properties

et al. 2012

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Protein adsorption is one of the key parameters influencing the biocompatibility of medical device materials. This study investigates serum protein adsorption and bacterial attachment on polymer coatings deposited using an atmospheric pressure plasma jet system. The adsorption of bovine serum albumin and bovine fibrinogen (Fg) onto siloxane and fluorinated siloxane elastomeric coatings that exhibit water contact angles (θ) ranging from superhydrophilic (θ < 5°) to superhydrophobic (θ > 150°) were investigated. Protein interactions were evaluated in situ under dynamic flow conditions by spectroscopic ellipsometry. Superhydrophilic coatings showed lower levels of protein adsorption when compared with hydrophobic siloxane coatings, where preferential adsorption was shown to occur. Reduced levels of protein adsorption were also observed on fluorinated siloxane copolymer coatings exhibiting hydrophobic wetting behaviour. The lower levels of protein adsorption observed on these surfaces indicated that the presence of fluorocarbon groups have the effect of reducing surface affinity for protein attachment. Analysis of superhydrophobic siloxane and fluorosiloxane surfaces showed minimal indication of protein adsorption. This was confirmed by bacterial attachment studies using a Staphylococcus aureus strain known to bind specifically to Fg, which showed almost no attachment to the superhydrophobic coating after protein adsorption experiments. These results showed the superhydrophobic surfaces to exhibit antimicrobial properties and significantly reduce protein adsorption.

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“…The practice to reduce unspecific protein adsorption by using albumin as a blocking molecule is based on the assumption that proteins adsorb in monolayers on surfaces and that they cannot adsorb on top of each other [10][11][12][13]. Thus, having a monolayer of albumin on a surface would prevent adsorption of fibrinogen and the only way fibrinogen can adsorb onto the blocked surface is by adsorbing in areas of the surface not covered with albumin or by displacing already adsorbed albumin molecules.…”

Section: Introductionmentioning

confidence: 99%

Fibrinogen adsorption on blocked surface of albumin

Holmberg

Hou

2011

Colloids and Surfaces B: Biointerfaces

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In-situ protein adsorption study on biofunctionalized surfaces using spectroscopic ellipsometry

Cited by 25 publications

References 36 publications

Yeast Cytochrome c Monolayer on Flat and Nanostructured Gold Films Studied by UV–Vis Spectroscopic Ellipsometry

Yeast Cytochrome c Monolayer on Flat and Nanostructured Gold Films Studied by UV–Vis Spectroscopic Ellipsometry

Evaluation of Protein Adsorption on Atmospheric Plasma Deposited Coatings Exhibiting Superhydrophilic to Superhydrophobic Properties

Fibrinogen adsorption on blocked surface of albumin

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