In Situ Polymerization of Monolith Based on Poly(Triallyl Isocyanurate-co-Trimethylolpropane Triacrylate) and Its Application in High-Performance Liquid Chromatography

Abstract

A novel organic monolithic stationary phase was prepared for high-performance liquid chromatography (HPLC) by in situ copolymerization. In which, triallyl isocyanurate (TAIC) and trimethylolpropane triacrylate (TMPTA) in a binary porogenic solvent consisting of polyethylene glycol 200 and 1, 2-propanediol were used. The resultant monoliths with different column properties (e.g., morphology and pressure) were optimized by adjusting the ratio of TMPTA/TAIC and the composition of porogenic solvent. The resulting poly(TAIC-co-TMPTA) monolith showed a relatively homogeneous structure, good permeability and mechanical stability. The chemical group of the monolith was assayed by the infrared spectra method, the morphology of monolithic material was studied by scanning electron microscopy and the pore size distribution was determined by a mercury porosimeter. A series of small molecules were used to evaluate the column performance in terms of hydrophobic mode. At an optimized flow rate of 1.0 mL min(-1), the theoretical plate number of analyte was >15,000 plates m(-1). These applications demonstrated that the monoliths could be successfully used as the stationary phase in conjunction with HPLC to separate small molecules from the mixture.

Cholesterol‐imprinted macroporous monoliths: Preparation and characterization

“…The efficiency of modern well packed columns is characterized with N measured in hundreds of thousands plates/m . For macroporous monoliths, differed from packed columns with extremely fast mechanism of mass transfer and, as a rule, much lower specific surface area, these values are usually poor and vary from several thousands to several tens of thousands plates/m . Despite the wide application of N and H values for characterization of column properties for mentioned modes of HPLC, the application of these parameters for imprinted columns characterized by pseudo‐affinity interactions between the molecule of interest and its imprints is not widespread.…”