In-situ optical assessment of rat epicardial kinematic parameters reveals frequency-dependent mechanic heterogeneity related to gender

Muzio, Francesco Paolo Lo; Rozzi, Giacomo; Rossi, Stefano; Gerbolés, Amparo Guerrero; Fassina, Lorenzo; Pelà, Giovanna; Luciani, Giovanni Battista; Miragoli, Michele

doi:10.1016/j.pbiomolbio.2019.05.003

Cited by 6 publications

(5 citation statements)

References 38 publications

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“…In this study, we trained two SML classifiers based on Vi.Ki.E., a contactless imaging technology developed by our laboratories to evaluate the RV kinematics [21][22][23]43]. We used the Vi.Ki.E.…”

Section: Discussionmentioning

confidence: 99%

Artificial Intelligence Supports Decision Making during Open-Chest Surgery of Rare Congenital Heart Defects

Muzio

Rozzi

Rossi

et al. 2021

JCM

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The human right ventricle is barely monitored during open-chest surgery due to the absence of intraoperative imaging techniques capable of elaborating its complex function. Accordingly, artificial intelligence could not be adopted for this specific task. We recently proposed a video-based approach for the real-time evaluation of the epicardial kinematics to support medical decisions. Here, we employed two supervised machine learning algorithms based on our technique to predict the patients’ outcomes before chest closure. Videos of the beating hearts were acquired before and after pulmonary valve replacement in twelve Tetralogy of Fallot patients and recordings were properly labeled as the “unhealthy” and “healthy” classes. We extracted frequency-domain-related features to train different supervised machine learning models and selected their best characteristics via 10-fold cross-validation and optimization processes. Decision surfaces were built to classify two additional patients having good and unfavorable clinical outcomes. The k-nearest neighbors and support vector machine showed the highest prediction accuracy; the patients’ class was identified with a true positive rate ≥95% and the decision surfaces correctly classified the additional patients in the “healthy” (good outcome) or “unhealthy” (unfavorable outcome) classes. We demonstrated that classifiers employed with our video-based technique may aid cardiac surgeons in decision making before chest closure.

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Section: Discussionmentioning

confidence: 99%

Artificial Intelligence Supports Decision Making during Open-Chest Surgery of Rare Congenital Heart Defects

Muzio

Rozzi

Rossi

et al. 2021

JCM

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“…Kinematic measurements of the beating rat hearts were performed using video kinematic evaluation of the epicardial movement 56 . Briefly, we used a computer-vision technology capable of acquiring videos of the epicardial motion during the sinus rhythm at 500 fps for 2 s (LabView).…”

Section: Methodsmentioning

confidence: 99%

Synthetic recovery of impulse propagation in myocardial infarction via silicon carbide semiconductive nanowires

et al. 2022

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Myocardial infarction causes 7.3 million deaths worldwide, mostly for fibrillation that electrically originates from the damaged areas of the left ventricle. Conventional cardiac bypass graft and percutaneous coronary interventions allow reperfusion of the downstream tissue but do not counteract the bioelectrical alteration originated from the infarct area. Genetic, cellular, and tissue engineering therapies are promising avenues but require days/months for permitting proper functional tissue regeneration. Here we engineered biocompatible silicon carbide semiconductive nanowires that synthetically couple, via membrane nanobridge formations, isolated beating cardiomyocytes over distance, restoring physiological cell-cell conductance, thereby permitting the synchronization of bioelectrical activity in otherwise uncoupled cells. Local in-situ multiple injections of nanowires in the left ventricular infarcted regions allow rapid reinstatement of impulse propagation across damaged areas and recover electrogram parameters and conduction velocity. Here we propose this nanomedical intervention as a strategy for reducing ventricular arrhythmia after acute myocardial infarction.

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“…According to this principle, the uncontracted length L 0 (pixel) of each cardiomyocyte was measured by ImageJ-Fiji (https://fiji.sc/, accessed on 7 March 2022). The movement of each cell tail was evaluated as previously described [20][21][22][23]. In this manner, it was possible to measure the mean maximum displacement (pixel) of both tails: MD 1 and MD 2 , respectively.…”

Section: Analysis Of Cell Shortening and Kinematics/dynamics Of Prima...mentioning

confidence: 99%

“…Subsequently, the cell fraction shortening (%) was calculated as (MD1 + MD2)/(L0/2) (Figure 1B). Similarly, the total contraction force (pixel/s 2 ) was calculated as the sum of the tails' force (each measured as previously reported [20][21][22][23][24]), whereas the mean contractility Similarly, the total contraction force (pixel/s 2 ) was calculated as the sum of the tails' force (each measured as previously reported [20][21][22][23][24]), whereas the mean contractility (pixel/s) and the mean contraction energy (pixel 2 /s 2 ) were calculated as the average of the tails' parameters (each measured as previously reported [20][21][22][23][24]).…”

Section: Analysis Of Cell Shortening and Kinematics/dynamics Of Prima...mentioning

confidence: 99%

Cell Shortening and Calcium Homeostasis Analysis in Adult Cardiomyocytes via a New Software Tool

et al. 2022

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Intracellular calcium (Ca2+) is the central regulator of heart contractility. Indeed, it couples the electrical signal, which pervades the myocardium, with cardiomyocytes contraction. Moreover, alterations in calcium management are the main factors contributing to the mechanical and electrical dysfunction observed in failing hearts. So, simultaneous analysis of the contractile function and intracellular Ca2+ is indispensable to evaluate cardiomyocytes activity. Intracellular Ca2+ variations and fraction shortening are commonly studied with fluorescent Ca2+ indicator dyes associated with microscopy techniques. However, tracking and dealing with multiple files manually is time-consuming and error-prone and often requires expensive apparatus and software. Here, we announce a new, user-friendly image processing and analysis tool, based on ImageJ-Fiji/MATLAB® software, to evaluate the major cardiomyocyte functional parameters. We succeeded in analyzing fractional cell shortening, Ca2+ transient amplitude, and the kinematics/dynamics parameters of mouse isolated adult cardiomyocytes. The proposed method can be applied to evaluate changes in the Ca2+ cycle and contractile behavior in genetically or pharmacologically induced disease models, in drug screening and other common applications to assess mammalian cardiomyocyte functions.

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In-situ optical assessment of rat epicardial kinematic parameters reveals frequency-dependent mechanic heterogeneity related to gender

Cited by 6 publications

References 38 publications

Artificial Intelligence Supports Decision Making during Open-Chest Surgery of Rare Congenital Heart Defects

Artificial Intelligence Supports Decision Making during Open-Chest Surgery of Rare Congenital Heart Defects

Synthetic recovery of impulse propagation in myocardial infarction via silicon carbide semiconductive nanowires

Cell Shortening and Calcium Homeostasis Analysis in Adult Cardiomyocytes via a New Software Tool

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