In situ monitoring of PTHLH secretion in neuroblastoma cells cultured onto nanoporous membranes

Escosura‐Muñiz, Alfredo de la; Espinoza-Castañeda, Marisol; Chamorro-García, Alejandro; Rodríguez-Hernández, Carlos J.; Torres, Carmen de; Merkoçi, Arben

doi:10.1016/j.bios.2018.01.064

Cited by 19 publications

(10 citation statements)

References 23 publications

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“…Moreover, elevated urine ORM1 was positively correlated with the clinicopathological parameters of BC, which indicated ORM1 as a potential biomarker in BC (44). PTHLH is a member of the parathyroid hormone family, which is responsible for most cases of humoral hypercalcemia of malignancy (45). PTHLH functions as a critical regulator of cellular and organ growth, development, migration, survival and of epithelial calcium ion transport (45).…”

Section: Discussionmentioning

confidence: 99%

“…PTHLH is a member of the parathyroid hormone family, which is responsible for most cases of humoral hypercalcemia of malignancy (45). PTHLH functions as a critical regulator of cellular and organ growth, development, migration, survival and of epithelial calcium ion transport (45). Recently, Chen et al revealed that silencing of transforming growth factor-b-activated kinase 1 (TAK1) in BC cells promote the development of cancer cells by upregulating PTHLH (46).…”

Section: Discussionmentioning

confidence: 99%

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A TP53-Associated Immune Prognostic Signature for the Prediction of Overall Survival and Therapeutic Responses in Muscle-Invasive Bladder Cancer

Cai

et al. 2020

Front. Immunol.

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BackgroundTP53 gene mutation is one of the most common mutations in human bladder cancer (BC) and has been implicated in the progression and prognosis of BC.MethodsRNA sequencing data and TP53 mutation data in different populations and platforms were downloaded from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) database to determine and validate a TP53-associated immune prognostic signature (TIPS) based on differentially expressed immune-related genes (DEIGs) between muscle-invasive bladder cancer (MIBC) patients with and without TP53 mutations.ResultsA total of 99 DEIGs were identified based on TP53 mutation status. TIPS including ORM1, PTHLH, and CTSE were developed and validated to identify high-risk prognostic group who had a poorer prognosis than low-risk prognostic group in TCGA and GEO database. The high-risk prognostic group were characterized by a higher abundance of regulatory T cells, myeloid-derived suppressor cells, and tumor-associated macrophages than the low-risk prognostic group. Moreover, they exhibited a lower abundance of CD56bright NK cells, higher expression of CTLA4, LAG3, PDCD1, TIGIT, and HAVCR2, as well as being more likely to respond to anti–PD-1, and neoadjuvant chemotherapy than the low-risk prognostic group. Based on TIPS and other clinical characteristics, a nomogram was constructed for clinical use.ConclusionTIPS derived from TP53 mutation status is a potential prognostic signature or therapeutic target but additional prospective studies are necessary to confirm this potential.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A TP53-Associated Immune Prognostic Signature for the Prediction of Overall Survival and Therapeutic Responses in Muscle-Invasive Bladder Cancer

Cai

et al. 2020

Front. Immunol.

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“…It is also worth highlighting the potential of these sensing systems for the in-situ monitoring of cell/bacteria secreted proteins, of high interest for the screening of anti-cancer drugs as well as for testing of novel antimicrobial/antivirulence agents [131,132].…”

Section: Nanoparticles As Blocking Agents In Nanochannels-based Immentioning

confidence: 99%

Nanoparticles as Emerging Labels in Electrochemical Immunosensors

Iglesias-Mayor

Amor-Gutiérrez

Costa-Garcı́a

et al. 2019

Sensors

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This review shows recent trends in the use of nanoparticles as labels for electrochemical immunosensing applications. Some general considerations on the principles of both the direct detection based on redox properties and indirect detection through electrocatalytic properties, before focusing on the applications for mainly proteins detection, are given. Emerging use as blocking tags in nanochannels-based immunosensing systems is also covered in this review. Finally, aspects related to the analytical performance of the developed devices together with prospects for future improvements and applications are discussed.

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“…SAMs of silanes with hydrophobic terminal groups increase the hydrophobicity of NAA [ 107 ], SAMs of perfluoroalkylchorosilanes reduce the effective pore diameter of NAA for molecular transport [ 108 ], poly(ethylene glycol)silane SAMs improve the biocompatibility of NAA for immunoisolation [ 109 ], and SAMs of mercaptosilanes have been used for sensing applications [ 110 , 111 , 112 , 113 ]. Similar to SAMs of alkanoic and phosphonic acids, SAMs of organosilanes can act as chemical linkers for further immobilization of biomolecules [ 114 , 115 , 116 ], polymers [ 116 ], and nanoparticles [ 117 ]. Furthermore, organosilanes containing different terminal functionalities (e.g., amine, thiol, carboxyl, etc.)…”

Section: Surface Modification Of Nanoporous Anodic Alumina Photonimentioning

confidence: 99%

Nanoporous Anodic Alumina Photonic Crystals for Optical Chemo- and Biosensing: Fundamentals, Advances, and Perspectives

et al. 2018

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Optical sensors are a class of devices that enable the identification and/or quantification of analyte molecules across multiple fields and disciplines such as environmental protection, medical diagnosis, security, food technology, biotechnology, and animal welfare. Nanoporous photonic crystal (PC) structures provide excellent platforms to develop such systems for a plethora of applications since these engineered materials enable precise and versatile control of light–matter interactions at the nanoscale. Nanoporous PCs provide both high sensitivity to monitor in real-time molecular binding events and a nanoporous matrix for selective immobilization of molecules of interest over increased surface areas. Nanoporous anodic alumina (NAA), a nanomaterial long envisaged as a PC, is an outstanding platform material to develop optical sensing systems in combination with multiple photonic technologies. Nanoporous anodic alumina photonic crystals (NAA-PCs) provide a versatile nanoporous structure that can be engineered in a multidimensional fashion to create unique PC sensing platforms such as Fabry–Pérot interferometers, distributed Bragg reflectors, gradient-index filters, optical microcavities, and others. The effective medium of NAA-PCs undergoes changes upon interactions with analyte molecules. These changes modify the NAA-PCs’ spectral fingerprints, which can be readily quantified to develop different sensing systems. This review introduces the fundamental development of NAA-PCs, compiling the most significant advances in the use of these optical materials for chemo- and biosensing applications, with a final prospective outlook about this exciting and dynamic field.

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In situ monitoring of PTHLH secretion in neuroblastoma cells cultured onto nanoporous membranes

Cited by 19 publications

References 23 publications

A TP53-Associated Immune Prognostic Signature for the Prediction of Overall Survival and Therapeutic Responses in Muscle-Invasive Bladder Cancer

A TP53-Associated Immune Prognostic Signature for the Prediction of Overall Survival and Therapeutic Responses in Muscle-Invasive Bladder Cancer

Nanoparticles as Emerging Labels in Electrochemical Immunosensors

Nanoporous Anodic Alumina Photonic Crystals for Optical Chemo- and Biosensing: Fundamentals, Advances, and Perspectives

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