In situ investigation of the oxidation of a phospholipid monolayer by reactive oxygen species

Fellows, Alexander P.; Casford, Michael T. L.; Davies, Paul B.

doi:10.1016/j.bpj.2022.10.040

Cited by 7 publications

(3 citation statements)

References 85 publications

(108 reference statements)

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“…Therefore, the difference in intensity was most likely linked to the effect of DES 4 on the different cell membrane structures of the treated bacteria. The same reason could be applied to the strength of the peak at 970-900 cm − 1 , which could be attributed to the disruption of phospholipids in both strains [63]. Owing to the dual nature of the phospholipid membrane, DES 4 hydrophobicity may cause dysfunction and cell rupture, which is supported by the FESEM results in the following section.…”

Section: Ftir Spectroscopy Analysismentioning

confidence: 62%

Antibacterial performance enhancement using hydrophobic deep eutectic solvents: COSMO-RS prediction, experimental validation, and synergistic action with antibiotics

Elgharbawy,

Warsi Khan,

Mohd Noor

et al. 2024

Journal of Molecular Liquids

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Section: Ftir Spectroscopy Analysismentioning

confidence: 62%

Antibacterial performance enhancement using hydrophobic deep eutectic solvents: COSMO-RS prediction, experimental validation, and synergistic action with antibiotics

Elgharbawy,

Warsi Khan,

Mohd Noor

et al. 2024

Journal of Molecular Liquids

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“…Oxidized derivatives of phosphatidylinositol, OxPI, are less well understood, with studies identifying the presence of OxPI in plants (Nilsson et al, 2014) but not yet able to determine specific function. The presence of oxidized phospholipids has been linked to oxidative damage and increases in ROS (Fellows et al, 2023), potentially impacting membrane structure and permeability, and leading to eventual cell apoptosis (Gaschler and Stockwell, 2017). OxPI and other oxidized phospholipids have not been well explored in algae but could indicate increases in ROS and oxidative stress due to temperature increase.…”

Section: Discussionmentioning

confidence: 99%

Diversity of lipid profiles of Symbiodiniaceae under temperature and nutrient stress

La Motta,

Padula,

Sommer

et al. 2024

Front. Protistol.

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Lipid-based survival mechanisms allow microalgae to occupy wide geographical ranges and survive abiotic stress. The protist Symbiodiniaceae are globally distributed from temperate to tropical environments, and establish mutualisms with numerous hosts, including cnidarians. The ability for these dinoflagellates to maintain cellular function under wide ranging environmental conditions will influence the survival and geographic distribution of their hosts. One mechanism that microalgae utilize to adapt to environmental changes is lipid remodeling, such as increased saturation of membranes to maintain the structural integrity under temperature changes, and lipid accumulation when nutrient availability decreases. Whether Symbiodiniaceae utilize lipid remodeling to adapt to sublethal environmental change is yet to be resolved. This study examines the effects of temperature (16°C to 31°C), and nitrogen (N) and phosphorus (P) availability, on the lipid composition and physiology of cultured Symbiodiniaceae (from genera Breviolum, Cladocopium and Durusdinium) isolated from temperate or tropical environments. Glycerolipids, particularly triacyclglycerols, increased while cell size decreased under N- and NP-nutrient limited cultures, across all Symbiodiniaceae species. P-limitation caused a decrease in phosphatidylcholine, an important membrane lipid, and saw an increase in isoprenol lipids. This suggests a diversion of phosphorus from phospholipid membranes to the biosynthesis of membrane-stabilizing isoprenes. Reduced photophysiology under P-limitation in all Symbiodiniaceae further supports evidence that P-limitation induced stress in these Symbiodiniaceae cells. As expected, growth rate was reduced in all Symbiodiniaceae at temperature extremes (31°C). Significant increases in oxidized lipids, particularly oxidized phosphatidylinositol, and a reduction in ether-linked phospholipids in cultures grown at 31°C, suggests increased reactive oxygen species (ROS) abundance in these cells. In addition, at 31 °C, D. trenchii and both C. goreaui spp. cell size increased, a common sign of ROS accumulation, cell cycle arrest and necrosis. The observed increases in lipid energy storage (triacylglycerols and isoprenoids) under nutrient stress, as well as ROS-mitigation via lipid remodeling leading to increases in saturated fatty acids and oxidized lipids under temperatures stress, suggest Symbiodiniaceae can remodel their lipids to adapt to environmental shifts. If similar mechanisms occur in hospite, this could be an adaptive strategy for coral holobionts under a changing climate.

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“…Several studies have reported on the altered surface properties and phase behavior of lipids using the Langmuir-Blodgett technique due to variations in temperature [15][16][17], different chain lengths of acyl lipids [18][19][20], compositions with other lipids [21][22][23], mixtures with other components [24][25][26] or particles [27][28][29], differences in subphases [30][31][32][33], oxidation and degradation [34][35][36], and pH changes [37][38][39]. Only a few research cases have thoroughly investigated hysteresis using the same technique, particularly for phospholipids, e.g., [6,29,[40][41][42][43][44].…”

Section: Introductionmentioning

confidence: 99%

Unraveling Complex Hysteresis Phenomenon in 1,2-Dipalmitoyl-sn-Glycero-3-Phosphocholine Monolayer: Insight into Factors Influencing Surface Dynamics

Sudjarwo,

Toca-Herrera

2023

IJMS

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This study explores the hysteresis phenomenon in DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine) monolayers, considering several variables, including temperature, compression and expansion rates, residence time, and subphase content. The investigation focuses on analyzing the influence of these variables on key indicators such as the π-A isotherm curve, loop area, and compression modulus. By employing the Langmuir–Blodgett technique, the findings reveal that all the examined factors significantly affect the aforementioned parameters. Notably, the hysteresis loop, representing dissipated energy, provides valuable insights into the monolayer’s viscoelasticity, molecular packing, phase transition changes, and resistance during the isocycle process. These findings contribute to a comprehensive understanding of the structural and dynamic properties of DPPC monolayers, offering insights into their behavior under varying conditions. Moreover, the knowledge gained from this study can aid in the development of precise models and strategies for controlling and manipulating monolayer properties, with potential applications in drug delivery systems, surface coatings, as well as further investigation into air penetration into alveoli and the blinking mechanism.

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In situ investigation of the oxidation of a phospholipid monolayer by reactive oxygen species

Cited by 7 publications

References 85 publications

Antibacterial performance enhancement using hydrophobic deep eutectic solvents: COSMO-RS prediction, experimental validation, and synergistic action with antibiotics

Antibacterial performance enhancement using hydrophobic deep eutectic solvents: COSMO-RS prediction, experimental validation, and synergistic action with antibiotics

Diversity of lipid profiles of Symbiodiniaceae under temperature and nutrient stress

Unraveling Complex Hysteresis Phenomenon in 1,2-Dipalmitoyl-sn-Glycero-3-Phosphocholine Monolayer: Insight into Factors Influencing Surface Dynamics

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