In situ immunogold labeling analysis of the rice hoja blanca virus nucleoprotein and major noncapsid protein

Espinoza, Ana M.; Hernández, Míriam Albert; Pereira, Ricardo; Falk, Bryce W.; Medina, Vicente

doi:10.1016/0042-6822(92)90237-j

Cited by 22 publications

(9 citation statements)

References 24 publications

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“…The FIs found in PPSMV-infected cells are possibly a nonstructural inclusion protein of PPSMV (30). Although tenuiviruses do not produce cellular inclusion bodies that resemble the MBBs found in PPSMV-infected cells, their nonstructural protein inclusions (NCP) in infected cells resemble the FIs of PPSMV (10,11,30). PPSMV also resembles tospoviruses, which share many properties with tenuiviruses (12).…”

Section: Discussionmentioning

confidence: 99%

A Novel Mite-Transmitted Virus with a Divided RNA Genome Closely Associated with Pigeonpea Sterility Mosaic Disease

Kumar¹,

Jones²,

Reddy³

2003

Phytopathology®

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Section: Discussionmentioning

confidence: 99%

A Novel Mite-Transmitted Virus with a Divided RNA Genome Closely Associated with Pigeonpea Sterility Mosaic Disease

Kumar¹,

Jones²,

Reddy³

2003

Phytopathology®

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“…The putative 94K protein (pc2) carries stretches of amino acid sequence showing weak similarity to parts of the membrane glycoproteins of Punta Toro virus, Rift Valley fever virus and Uukuniemi virus, all three in the genus Phlebovirus, suggesting an evolutionary relationship between the animal phleboviruses and the plant tenuiviruses [11,18]. Thus, tenuiviruses might be expected to have the enveloped virion form of Bunyaviruses but no such form has so far been found in either infected plants or insects [19][20][21][22][23]. The analogous Phlebovirus protein is processed into two glycoproteins, G1 of 72 kDa from the N-terminal part and G2, a 67 kDa protein from the C-terminal part.…”

Section: Introductionmentioning

confidence: 95%

Detection and Localization of Rice stripe virus Gene Products In Vivo

Liang

et al. 2005

Virus Genes

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The genome of the Tenuivirus, Rice stripe virus (RSV) comprises four RNAs, the smallest three of which each contain two open reading frames (ORFs) arranged in an ambisense manner. The expression of the ORFs from RNAs 2-4 in plants and the insect vector, Laodelphax striatellus, was studied using antisera raised against the gene products. In Western blotting of the proteins from infected plants, the molecular masses of p2, p3, pc3 (nucleocapsid protein, N) and p4 (major non-structural protein, NCP) were as expected; that of pc4 appeared larger than expected. Antisera to the N- and C-terminal parts of the complementary ORF on RNA 2, analogous to that encoding glycoproteins on genomes of bunyaviruses and tospoviruses, revealed banding patterns suggestive of processing of the product; the possible processing is discussed. Four types of inclusion bodies were identified by immunofluorescent and immunogold microscopy of thin sections of infected leaves. Most electron-dense amorphous semi-electron-opaque inclusion bodies (dASO) contained only p4 while some contained at least p2, pc2-N, p3, pc3 as well as p4. A ring-like structure containing at least pc2-N, p4 and pc4 was also identified in infected plant cells. Fibrillar amorphous semi-electron-opaque inclusion bodies (fASO) contained only p4. Filamentous electron-opaque inclusion bodies (FEO), which consist of pc2-N(.)and p4, were found both in infected plant cells and in the mid-gut lumen and mid-gut epithelial cells of L. striatellus. This suggests an interaction between p4 and pc2-N and a function of pc2-N distinct from that of its-homologue in Bunyaviridae. Our results confirm the in vivo ambisense coding strategy of Tenuivirus RNA 2 and provide further evidence that RSV does not produce enveloped virions in infected rice plants.

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“…For RHBV, Morales & Niessen (1985) reported both 3 nm-and 8 nm-wide filaments that may adopt helical or loose configurations. Mostly circular RHBV RNPs, 8 nm wide and of variable lengths were also observed (Espinoza et al, 1992(Espinoza et al, , 1993. For MStV, 3 nm-wide filaments (Gingery et al, 1981), but also circular and 8 nm-wide rod-shaped forms have been described (reviewed in Gingery, 1988).…”

Section: Morphology and Structural Componentsmentioning

confidence: 99%