In situ identification of symbiotic dinoflagellates, the genus Symbiodinium with fluorescence-labeled rRNA-targeted oligonucleotide probes

Yokouchi, Hiroko; Takeyama, Haruko; Miyashita, Hideaki; Maruyama, Tadashi; Matsunaga, Tadashi

doi:10.1016/s0167-7012(02)00250-6

Cited by 20 publications

(10 citation statements)

References 32 publications

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“…The fluorochrome CY3 was chosen as the fluorescent label for the oligonucleotide probes, for a number of reasons, in spite of the fact that the emission signature for this dye is similar to the autofluorescence signature of chlorophyll. CY3 has been successfully used for FISH protocols with other algae, including dinoflagellates (Yokouchi et al, 2003). It is relatively easy to decolorize cells and reduce the background levels of chlorophyll autofluorescence to a degree that does not interfere with visualization of the probe.…”

Section: Fluorescence In Situ Hybridizationmentioning

confidence: 99%

Localization of polyketide synthase encoding genes to the toxic dinoflagellate Karenia brevis

et al. 2005

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Karenia brevis is a toxic marine dinoflagellate endemic to the Gulf of Mexico. Blooms of this harmful alga cause fish kills, marine mammal mortalities and neurotoxic shellfish poisonings. These harmful effects are attributed to a suite of polyketide secondary metabolites known as the brevetoxins. The carbon framework of all polyketides is assembled by a polyketide synthase (PKS). Previously, PKS encoding genes were amplified from K. brevis culture and their similarity to a PKS gene from the closely related protist, Cryptosporidium parvum, suggested that these genes originate from the dinoflagellate. However, K. brevis has not been grown axenically. The associated bacteria might be the source of the toxins or the PKS genes. Herein we report the localization of PKS encoding genes by a combination of flow cytometry/PCR and fluorescence in situ hybridization (FISH). Two genes localized exclusively to K. brevis cells while a third localized to both K. brevis and associated bacteria. While these genes have not yet been linked to toxin production, the work describes the first definitive evidence of resident PKS genes in any dinoflagellate.

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Section: Fluorescence In Situ Hybridizationmentioning

confidence: 99%

Localization of polyketide synthase encoding genes to the toxic dinoflagellate Karenia brevis

et al. 2005

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“…Walsh et al, 1998;Penna and Magnani, 1999), Symbiodinium spp. (Yokouchi et al, 2003) and Amoebophrya spp. (Gunderson et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Large subunit ribosomal RNA gene variation and sequence heterogeneity of Dinophysis (Dinophyceae) species from Scottish coastal waters

Hart¹,

Green²,

Bresnan³

et al. 2007

Harmful Algae

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“…Coral tissues are highly fluorescent due to the presence of pocilloporins (green fluorescent protein-like molecules) (14,15,34) and high densities of endosymbiotic dinoflagellates containing chlorophyll (22,42). This extensive autofluorescence confounds coral microbial FISH studies, as image analysis is unable to resolve the sources of fluorescence (12).…”

mentioning

confidence: 99%

Fluorescence In Situ Hybridization and Spectral Imaging of Coral-Associated Bacterial Communities

Ainsworth

Fine

Blackall

et al. 2006

Appl Environ Microbiol

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In situ identification of symbiotic dinoflagellates, the genus Symbiodinium with fluorescence-labeled rRNA-targeted oligonucleotide probes

Cited by 20 publications

References 32 publications

Localization of polyketide synthase encoding genes to the toxic dinoflagellate Karenia brevis

Localization of polyketide synthase encoding genes to the toxic dinoflagellate Karenia brevis

Large subunit ribosomal RNA gene variation and sequence heterogeneity of Dinophysis (Dinophyceae) species from Scottish coastal waters

Fluorescence In Situ Hybridization and Spectral Imaging of Coral-Associated Bacterial Communities

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