In situ hybridization analysis of chromosomal homologies in Drosophila melanogaster and Drosophila virilis.

Abstract

Twenty-four biotin-labeled recombinant-DNA probes which contained putative unique-sequence Drosophila melanogaster DNA were hybridized to larval salivary-gland chromosomes of D. melanogaster and Drosophila virilis. All probes hybridized to D. melanogaster chromosomes at the expected sites. However, one probe hybridized to at least 16 additional sites, and one hybridized to one additional site. Thirteen probes hybridized strongly to D. virilis chromosomes, four hybridized weakly and infrequently, and seven did not hybridize. Probes representing two multigene families (beta-tubulin and yolk-protein) hybridized as would be expected if all sites had been conserved in the two species on the same chromosomal elements. The multiple hybridization sites of a third probe which may represent a multigene family were also conserved. The results were consistent with H.J. Muller's proposal that chromosomal elements have been conserved during evolution of this genus.

The evolution of chromosomal sex determination and dosage compensation

“…The observed rate of decline in fitness of a nonrecombining pair of chromosomes in a small laboratory population of D. melanogaster also indicates a high mutation rate to deleterious alleles [42]. Chromosome arms in the genus Drosophila are of similar size, and seem to be well conserved in evolution [43][44][45]. The mutation rate both for the primeval Y and for a neo-Y formed by an X-autosome or Y-autosome fusion (Fig.…”

Protocol for preparing Drosophila genomic DNA to create chromosome-level de novo genome assemblies

“…However, for Drosophila it is possible to use short-read libraries since the chromosome arms are conserved. 21 , 22 , 23 , 24 Hi-C is still recommended for obtaining better resolution of the non-coding regions of Drosophila genomes. Here we use Illumina sequencing technology to sequence a whole genome DNA preparation in combination with transcriptomics data to polish the genome assembly.…”

Βιοχημικη Και Γενετικη Αναλυση Της Αλκαλικης Φωσφατασης Κατα Την Αναπτυξη Της Ceratitis Capitata

“…Η ομοιότητα στη γονιδιακή οργάνωση δεν υπάρχει μόνο μεταξύ της Drosophila melanogaster και της Ceratitis capitata αλλά παρατηρήθηκε και μεταξύ της Drosophila melanogaster και της Drosophila subobscura (Loukas et al, 1979• Loukas andVergini, 1985). Επίσης ομοιότητα στη γονιδιακή οργάνωση έχει παρατηρηθεί μεταξύ της Lucillia cuprina dorsalis και των Musca domestica και Drosophila melanogaster (Foster et al, 1981), μεταξύ της Drosophila melanogaster και Drosophila virilis (Whiting et al, 1990), μεταξύ της Drosophila miranda και Drosophila melanogaster (Steinman, 1982), μεταξύ των διαφόρων ειδών της ομάδας obscura του γένους Drosophila (Steinman et al, 1984), μεταξύ των ομάδων melanogaster και obscura του γένους Drosophila Kafatos, 1986• 1988) και τέλος μεταξύ διαφόρων ειδών διπτέρων .…”