In-situ high-precision surface topographic and Raman mapping by divided-aperture differential confocal Raman microscopy

Wu, Hanxu; Su, Yunhao; Qiu, Lirong; Ni, He; Xu, Ke-Mi; Zhao, Wenguang

doi:10.1016/j.apsusc.2021.149061

Cited by 9 publications

(4 citation statements)

References 44 publications

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“…1c ), thereby achieving high-precision axial focusing to ensure that the focal point of each scanning position is focused on the sample surface, and the spot size is the smallest at this time, which can achieve the optimal spatial resolution of the system (Fig. 1d ) 33 . We used a normal silver mirror and a single-crystal silicon wafer to test the axial focusing capability of the system.…”

Section: Resultsmentioning

confidence: 99%

A high-precision multi-dimensional microspectroscopic technique for morphological and properties analysis of cancer cell

Qiu

et al. 2023

Light Sci Appl

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Raman and Brillouin scattering are sensitive approaches to detect chemical composition and mechanical elasticity pathology of cells in cancer development and their medical treatment researches. The application is, however, suffering from the lack of ability to synchronously acquire the scattering signals following three-dimensional (3D) cell morphology with reasonable spatial resolution and signal-to-noise ratio. Herein, we propose a divided-aperture laser differential confocal 3D Geometry-Raman-Brillouin microscopic detection technology, by which reflection, Raman, and Brillouin scattering signals are simultaneously in situ collected in real time with an axial focusing accuracy up to 1 nm, in the height range of 200 μm. The divided aperture improves the anti-noise capability of the system, and the noise influence depth of Raman detection reduces by 35.4%, and the Brillouin extinction ratio increases by 22 dB. A high-precision multichannel microspectroscopic system containing these functions is developed, which is utilized to study gastric cancer tissue. As a result, a 25% reduction of collagen concentration, 42% increase of DNA substances, 17% and 9% decrease in viscosity and elasticity are finely resolved from the 3D mappings. These findings indicate that our system can be a powerful tool to study cancer development new therapies at the sub-cell level.

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Section: Resultsmentioning

confidence: 99%

A high-precision multi-dimensional microspectroscopic technique for morphological and properties analysis of cancer cell

Qiu

et al. 2023

Light Sci Appl

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“…Confocal Raman microscopy (CRM) is a non-invasive method for locating and examining lipids, pigments, and carbohydrates in the algal research (Scalfi-Happ et al, 2011;Sharma et al, 2015). It allows real-time monitoring and examination of living cells without labeling; thus, Raman gives extensive chemical information without damaging or labeling samples (Zhang et al, 2021).…”

Section: Strain Optimization Through Uv Mutagenesismentioning

confidence: 99%

Enhancing algal production strategies: strain selection, AI-informed cultivation, and mutagenesis

Alzahmi,

Daakour,

Nelson

et al. 2024

Front. Sustain. Food Syst.

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Microalgae are emerging as a sustainable source of bioproducts, including food, animal feed, nutraceuticals, and biofuels. This review emphasizes the need to carefully select suitable species and highlights the importance of strain optimization to enhance the feasibility of developing algae as a sustainable resource for food and biomaterial production. It discusses microalgal bioprospecting methods, different types of cultivation systems, microalgal biomass yields, and cultivation using wastewater. The paper highlights advances in artificial intelligence that can optimize algal productivity and overcome the limitations faced in current microalgal industries. Additionally, the potential of UV mutagenesis combined with high-throughput screening is examined as a strategy for generating improved strains without introducing foreign genetic material. The necessity of a multifaceted optimization approach for enhanced productivity is acknowledged. This review provides an overview of recent developments crucial for the commercial success of microalgal production.

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“…In that way, it is possible also to observe structural differences between sections of the material and the character of the interphases. Moreover, many microspectrometers are confocal and allow in-depth measurements without special sample preparation [7].…”

Section: Introductionmentioning

confidence: 99%

Surface and in-depth structural changes in nuclear graphite irradiated with noble gases described with Raman imaging

Gawęda¹,

Wilczopolska²,

Suchorab³

et al. 2023

Nuclear Instruments and Methods in Physics Research Section B:

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In-situ high-precision surface topographic and Raman mapping by divided-aperture differential confocal Raman microscopy

Cited by 9 publications

References 44 publications

A high-precision multi-dimensional microspectroscopic technique for morphological and properties analysis of cancer cell

A high-precision multi-dimensional microspectroscopic technique for morphological and properties analysis of cancer cell

Enhancing algal production strategies: strain selection, AI-informed cultivation, and mutagenesis

Surface and in-depth structural changes in nuclear graphite irradiated with noble gases described with Raman imaging

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