In situ growth of hybrid nanoflowers on activated carbon fibers as electrodes for mediatorless enzymatic biofuel cells

Vo, Thuan Ngoc; Tran, Tai Duc; Nguyen, Hoang; Kong, Do Yun; Kim, Moon Il; Kim, Il Tae

doi:10.1016/j.matlet.2020.128662

Cited by 13 publications

(11 citation statements)

References 13 publications

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“…Nanoflowers, synthesized from the coordination of natural enzymes and inorganic metal ions followed by anisotropic growth to yield flowerlike morphology under mild conditions, have been employed to construct electrodes for EBFCs. , Enzyme-incorporated hybrid nanoflowers have high specificity toward the corresponding substrate with high activity and stability, which are appropriate for preparing the electrodes of EBFCs. On the other hand, the nanoflowers themselves exhibit low electrical conductivity, limiting their independent utilization to prepare the enzyme electrodes and forcing one to prepare the electrodes with the help of another conductive nanomaterial such as CNT . Advances in the design of complex hybrid nanostructures for enzyme electrodes may improve the performance of EBFCs.…”

Section: Roles Of Nanostructured Supports In Ebfcsmentioning

confidence: 99%

“…With the EBFC, the power output reached 200 μW cm –2 , an OCV of 0.49 V, and a current density of 1.62 mA cm –2 , with an extended lifetime to maintain over 90% of the initial power output over 2 months during storage at 4 °C. Vo et al grew hybrid nanoflowers in situ on the surface of activated carbon nanofibers to provide conductivity to the nanoflowers (Figure A) . GOx- and CAT-nanoflowers were used to construct the anode, and LAC-nanoflowers were used to prepare the cathode.…”

Section: Recent Research On Nanostructured Support-based Ebfcsmentioning

confidence: 99%

“…On the other hand, the nanoflowers themselves exhibit low electrical conductivity, limiting their independent utilization to prepare the enzyme electrodes and forcing one to prepare the electrodes with the help of another conductive nanomaterial such as CNT. 61 Advances in the design of complex hybrid nanostructures for enzyme electrodes may improve the performance of EBFCs. Nanostructured supporting materials have been crucial for improving the performance of EBFCs; their recent applications in generating electric power from biofuels are presented below.…”

Section: Roles Of Nanostructured Supports In Ebfcsmentioning

confidence: 99%

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Tailoring Nanostructured Supports to Achieve High Performance in Enzymatic Biofuel Cells

Kong

et al. 2022

ACS Appl. Energy Mater.

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Enzymatic biofuel cells (EBFCs) are attractive power sources capable of utilizing biomolecules to generate electrical power. However, their practical utility is limited because of their relatively low power output and short lifetime, which arise from the low enzyme loading capacity on the support materials, activity loss during enzyme immobilization, intrinsically poor enzyme stability, and limited substrate and electron transport. Nanobiocatalytic approaches, in which enzymes are incorporated with nanostructured supports, have been recognized as a potent solution to resolve these limitations. They construct high-performance enzyme electrodes utilizing the affirmative characteristics of the materials coupled with appropriate immobilization strategies. In this review, the roles of nanostructured supports and catalytic mechanisms for generating biofuel-driven electrical power, the recent research progress on EBFCs based on widely investigated nanocarbons, nanoparticles, and polymeric nanofibers, and newly emerged metal–organic frameworks, nanoflowers, and other nanohybrids are discussed. The current challenges and prospects for utilizing nanostructured supports in EBFCs are also discussed.

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Section: Roles Of Nanostructured Supports In Ebfcsmentioning

confidence: 99%

Section: Recent Research On Nanostructured Support-based Ebfcsmentioning

confidence: 99%

Section: Roles Of Nanostructured Supports In Ebfcsmentioning

confidence: 99%

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Tailoring Nanostructured Supports to Achieve High Performance in Enzymatic Biofuel Cells

Kong

et al. 2022

ACS Appl. Energy Mater.

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“…Catalase-like nanozymes cannot be utilized solely for glucose biofuel cell development; however, bi-enzymatic nanozymes showing GOx and catalase have been reported to serve as an anode in glucose biofuel cells [ 45 , 46 ]. In this regard, finding efficient catalase-like nanozymes or bi-enzymatic nanozymes showing both GOx and catalase-like activity, for preparing an efficient anode in glucose biofuel cells is crucial for decomposing the byproduct H 2 O 2 and enhancing the power output and extending the lifetime of glucose-based NBFCs.…”

Section: Synthetic Strategies and Catalytic Characteristics Of Nanozymes To Replace Natural Enzymes In Glucose Biofuel Cellsmentioning

confidence: 99%

Research Progress and Prospects of Nanozyme-Based Glucose Biofuel Cells

Kim

2021

Nanomaterials

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The appearance and evolution of biofuel cells can be categorized into three groups: microbial biofuel cells (MBFCs), enzymatic biofuel cells (EBFCs), and enzyme-like nanomaterial (nanozyme)-based biofuel cells (NBFCs). MBFCs can produce electricity from waste; however, they have significantly low power output as well as difficulty in controlling electron transfer and microbial growth. EBFCs are more productive in generating electricity with the assistance of natural enzymes, but their vulnerability under diverse environmental conditions has critically hindered practical applications. In contrast, because of the intrinsic advantages of nanozymes, such as high stability and robustness even in harsh conditions, low synthesis cost through facile scale-up, and tunable catalytic activity, NBFCs have attracted attention, particularly for developing wearable and implantable devices to generate electricity from glucose in the physiological fluids of plants, animals, and humans. In this review, recent studies on NBFCs, including the synthetic strategies and catalytic activities of metal and metal oxide-based nanozymes, the mechanism of electricity generation from glucose, and representative studies are reviewed and discussed. Current challenges and prospects for the utilization of nanozymes in glucose biofuel cells are also discussed.

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“…They are easily synthesized under mild conditions, from the coordination between amine/amide moieties of organic components and inorganic ions including copper, zinc, manganese, cobalt, and iron, followed by anisotropic growth to form highly branched flower-like morphologies. Various enzymes were incorporated within the nanoflowers to achieve improved catalytic properties and extensively applied to biosensor, bioremediation, biofuel cell, and other bioconversions [ 22 , 23 , 24 ]. However, the nanoflowers have not been widely investigated to seek their enzyme-mimicking activities, except for several peroxidase and laccase-mimicking cases [ 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Dual-Functional Peroxidase-Copper Phosphate Hybrid Nanoflowers for Sensitive Detection of Biological Thiols

Kim

2021

IJMS

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An effective strategy to detect biological thiols (biothiols), including glutathione (GSH), cysteine (Cys), and homocysteine (Hcy), holds significant incentive since they play vital roles in many cellular processes and are closely related to many diseases. Here, we demonstrated that hybrid nanoflowers composed of crystalline copper phosphate and horseradish peroxidase (HRP) served as a functional unit exhibiting dual catalytic activities of biothiol oxidase and HRP, yielding a cascade reaction system for a sensitive one-pot fluorescent detection of biothiols. The nanoflowers were synthesized through the anisotropic growth of copper phosphate petals coordinated with the amine/amide moieties of HRP, by simply incubating HRP and copper(II) sulfate for three days at room temperature. Copper phosphates within the nanoflowers oxidized target biothiols to generate H2O2, which activated the entrapped HRP to oxidize the employed Amplex UltraRed substrate to produce intense fluorescence. Using this strategy, biothiols were selectively and sensitively detected by monitoring the respective fluorescence intensity. This nanoflower-based strategy was also successfully employed for reliable quantification of biothiols present in human serum, demonstrating its great potential for clinical diagnostics.

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In situ growth of hybrid nanoflowers on activated carbon fibers as electrodes for mediatorless enzymatic biofuel cells

Cited by 13 publications

References 13 publications

Tailoring Nanostructured Supports to Achieve High Performance in Enzymatic Biofuel Cells

Tailoring Nanostructured Supports to Achieve High Performance in Enzymatic Biofuel Cells

Research Progress and Prospects of Nanozyme-Based Glucose Biofuel Cells

Dual-Functional Peroxidase-Copper Phosphate Hybrid Nanoflowers for Sensitive Detection of Biological Thiols

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