in situ Fabrication of Biological Chitosan and Gelatin-Based Hydrogels Loading Biphasic Calcium Phosphate Nanoparticles for Bone Tissue Regeneration

Abstract

Adjustably biodegradable materials have gained much attention in biomedical applications. Among of them, various hydrogel-based scaffolds have applied for regenerating soft and hard tissues. In this study, according to differently biological properties of gelatin or chitosan as well as biphasic calcium phosphate nanoparticles (BCPNPs), several injectable nanocomposite hydrogels (INgel) were enzymatically fabricated from a phenolic chitosan derivative (PCD), phenolic gelatin derivative (PGD) and BCPNPs. According the change of H2O2 concentration with follow-up the time, the in situ formation of INgel was varied from 35 to 80 s. The degradation rate of the nanocomposite materials significantly related to in presence of collagenase that expended from 3 days to over one month depending on amount of the formulated PCD. The BCPNPs-encapsulated PCD-PGD INgel enhanced mineralization in the simulated biofluid. Fluorescent cytotoxicity assay indicated that the INgel was fabricated from a higher amount of the PGD resulting in a significant proliferation of bone marrow mesenchymal stem cells. These preliminary results exhibited a great potential of the INgel for bone regeneration.

A β-(1-4) linked polysaccharide (Chitosan) in tripolyphosphate-zinc oxide (CTPP-ZnO) eco-conductive membrane

“…Although, in ῾protein-polysaccharide world᾿ there are several kinds of protein (gelatin) as well as polysaccharides such as cellulose, chitosan, dextran, heparin, inulin and chondroitin sulphate etc. have been potential importancy in hydrogel mediated biomedical-engineering application [4] , due to their practical performance such as delivery of bioactive-selective components, regeneration of tissue cell as well as encapsulation of nanoparticles [5] . Recent studies has been carried out the effort in to membrane synthesis from natural polymers, for example, the membranes made from polysaccharide such as cellulose [6,7] , but ought chitosan based [8,9] .…”

Introductory of Scherrer’s Equation in Laminated Plate-Shaped Hexagonal Hydroxyapatite Nanocrystals System

“…(OH) 2 which is the main inorganic components of hard tissue such as bone and teeth and they are used in medicinal application have attracted a great attention including several application such as artificial organs, tissue engineering, medical devices & dentistry etc [2,3]. Although ,fabricated biological hydrogels loaded biphasic calcium phosphate nanoparticles have also been reported for bone tissue regeneration [4]. ) in the hydrated layers can be substituted or incorporated by dicarboxylate such as succinate ions into OCP crystal structure has been reported [14-17].…”