In Situ Enzyme‐Induced Self‐Assembly of Antimicrobial‐Antioxidative Peptides to Promote Wound Healing

Abstract

Antimicrobial peptides (AMPs) with dual intrinsic antibacterial and antioxidative functions have emerged as promising choice to cure infected wound. However, the most widely applied approach to endow AMPs with antioxidative function is to combine with antioxidative moieties, which may affect the spatial structure and physiological stability of AMPs. Herein, a new type of AMPs with inherent desired stability, antibacterial activity, and reactive oxygen species (ROS) scavenging is developed to effectively heal the infected wound. This formulation is in situ formed at wound site by tyrosinase‐triggered oxidation and self‐assembly of lyophilized antimicrobial peptide Trp‐Arg‐Trp‐Arg‐Trp‐Tyr, providing enhanced stability and a fourfold and sevenfold increasement in antibacterial efficiency against E. coli and S. aureus compared to peptide monomers. The antimicrobial peptide is first oxidized and then assembled into nanoparticles. The melanin‐like structure has been demonstrated with efficient antioxidant properties, and the experimental data show that peptide nanoparticles to scavenge superoxide radicals, hydroxyl radicals, and H2O2. In vivo experiments confirmed that peptide nanoparticles effectively heal infected wounds and obviously reduce ROS. Overall, the research provides a new approach to formulating antimicrobial peptides to treat wound with high healing efficiency.

Synthesizing biomaterials in living organisms

“…28B). 158 The formed melanin-like AMP could efficiently reduce the wound-site ROS and promote healing.…”

Imaging‐Guided Antibacterial Based on Gold Nanocrystals and Assemblies

“…AMP is a kind of cationic small molecule polypeptide produced by organisms with strong antibacterial effect, which is an important component of the innate immunne system of organisms. [ 24 ] AMP has a unique antibacterial mechanism and is not easy to produce drug resistance. Studies have shown that antimicrobial peptides generally act through membrane destruction mechanisms and intracellular mechanisms.…”

Three-Pronged Flower-like Nanoplatforms for the Photothermal/Photodynamic/Quaternary Ammonium Salt Synergistic Antibacterial Method and Bioimaging

“…Bacterial infection has been a severe worldwide threat to public safety and health because of emerging antibiotic resistance. − Multidrug resistance is easily sparked by conventional treatment methods. , Thus, creating a unique treatment for damaged skin, organ tissues, and mucous membranes is a great challenge for medical researchers. One of the most efficient sterilization techniques is antibacterial photocatalytic treatment (APCT), a substitute sterilizing technology that has garnered considerable interest.…”