In Situ Detection of Polyphenols in Plant Microorganism Interactions

Andary, Claude; Mondolot-Cosson, L.; Dai, Guang

doi:10.1007/978-94-009-0189-6_3

Cited by 10 publications

(4 citation statements)

References 22 publications

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“…Chlorogenic acid is known to affect nematode coordination (Huang and Rhode, 1973). According to Andary et al (1996) and Schneider and Ullrich (1994), blue fluorescence reveals the presence of lignified cells, an active plant response to penetration or establishment of the pathogen, or both. Other authors have observed a blue, yellow or orange fluorescence in roots of resistant root-knot nematodes (Albuquerque et al 2010;Motta et al 2012), but a pale blue-white fluorescence under UV light in susceptible roots (Pegard et al, 2005), which is in contradiction with the observation done here in oat.…”

Section: Discussionmentioning

confidence: 99%

Histological characterization of resistance to Meloidogyne incognita in Avena sativa

Marini

Garbuglio

Dorigo

et al. 2016

Trop. plant pathol.

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Root-knot nematodes, especially Meloidogyne incognita, are recognized by expressive damages on important agronomic crops worldwide. White oat (Avena sativa) is an excellent alternative to sustainable management of nematodes especially when used in crop rotation with another plant species. In this context, the aim of the present work was to study the resistance of IPR Afrodite oat cultivar to M. incognita, using histopathological methods in a comparative approach with the susceptible cultivar URSFAPA Slava. Experiments were done under greenhouse conditions, with 6 replicates for each cultivar and evaluation time. Observations of fuchsinstained roots at 3, 9, 15 and 30 days after inoculation (DAI) revealed that the resistant cultivar did not suppress nematode penetration but caused a delay in the nematode development compared with the susceptible cultivar. Moreover, mature, egg-laying females were not found in the roots of the resistant cultivar. Hypersensitive reaction (HR) was observed at 18 DAI. The HR was characterized by the formation of few giant cells of reduced size on the resistant cultivar, associated with collapsed M. incognita specimens. In most specimens, light blue fluorescence indicated lignified cells, an active plant response to nematode penetration or establishment. Intercellular spaces were of dark yellow color, indicating the presence of phenols, often associated with plant defense mechanisms to nematodes and other pathogens. Our results suggest that resistance of IPR Afrodite to M. incognita is mediated by a HR response and studies are needed to clarify the nature of compounds produced by the plant involved in the resistance.

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Section: Discussionmentioning

confidence: 99%

Histological characterization of resistance to Meloidogyne incognita in Avena sativa

Marini

Garbuglio

Dorigo

et al. 2016

Trop. plant pathol.

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“…Phenolic compounds are abundant in the epidermis and to a lesser extent in the hypodermis, though not in the cortex ( Peterson et al ., 1978 ). Phenolics are known to inhibit extracellular enzymes of pathogens ( Andrary et al ., 1996 ). Studies of the permeability of epidermal and hypodermal layers also indicated that resistance of the apoplastic route increases with molecular dimensions of the permeant solutes ( Clarkson et al ., 1978 ).…”

Section: Discussionmentioning

confidence: 99%

Histology of Sclerotium cepivorum infection of onion roots and the spatial relationships of pectinases in the infection process

Metcalf¹,

Wilson

1999

Plant Pathology

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Sclerotium cepivorum (isolate Sc4) hyphae penetrated the epidermis and hypodermis of onion roots and grew into the cortex. Immediately following penetration only the cells through which S. cepivorum grew were lysed, but subsequently cells were killed and cell walls disintegrated ahead of the infection hyphae. Sclerotium cepivorum produced two polygalacturonases (PG) and two pectinesterases (PE) in culture. These isozymes were also found in infected onion root tissues and another PG and a PE were occasionally detected. Two isozymes of PG and three isozymes of PE diffused ahead of the infection hyphae. The spatial distribution of these enzymes was associated with cell death and cell wall degradation. The epidermis, hypodermis, endodermis and vascular tissues were more resistant to hydrolysis than the cortex, but only the endodermis and cells within it retained nuclei following hydrolysis of the surrounding cortical tissues. The cavity within the root cortex became ®lled with swollen, vacuolate S. cepivorum hyphae.

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“…Seed sections of 8‐μm thickness were generated using a LM2135 microtome (Leica Microsystems), dried and flattened on slide glass. The sections were stained with toluidine blue O (Réactifs‐RAL) to determine seed structure (Trump, Smuckler, & Benditt, ) and with phloroglucinol–HCl (1,3,5‐trihydroxybenzene; Sigma‐ALdrich, St. Louis) to label lignin (Andary, Mondolot‐Cosson, & Dai, ). For staining, the slides were immersed in 0.5% toluidine blue O for 1.5 min, rinsed with distilled water and air‐dried or stained with phloroglucinol reagent for 30 min and regressed with HCl, and air‐dried (Cadot, Minana‐Castello, & Chevalier, ).…”

Section: Methodsmentioning

confidence: 99%

Anatomical, biochemical and transcriptome analyses of Vitis vinifera cv. 'Hongju' reveal novel information regarding the seed hardness of stenospermocarpic soft‐seed grapes

et al. 2019

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The breeding of new stenospermocarpic seedless grape cultivars is of particular importance to the growing table grape market and grape processing industry. However, the developmental and genetic architectures of soft‐seed and seedlessness traits are unclear. In this study, we performed anatomical, histochemical and gene expression analyses to characterize the morphological and genetic basis of the seed hardness of 'Hongju', a stenospermocarpic soft‐seed grape cultivar developed by crossbreeding maternal 'Italia' and paternal 'Perlon' cultivars. We found intensively lignified medium integument layers in the seeds of hard‐seed cultivars, but these layers were degraded in the stenospermocarpic seedless cultivars beginning 35 days after flowering. The expression of genes related to the lignin and cellulose biosynthesis pathways was downregulated, whereas that of several cell‐wall loosening‐related genes was upregulated in 'Hongju' compared with 'Italia' beginning at 21 days after flowering. These results suggest that transcriptional suppression of the lignin and cellulose biosynthesis pathways coupled with reduced formation of medium integument plays an important role in the development of soft seeds in stenospermocarpic seedless grape cultivars.

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In Situ Detection of Polyphenols in Plant Microorganism Interactions

Cited by 10 publications

References 22 publications

Histological characterization of resistance to Meloidogyne incognita in Avena sativa

Histological characterization of resistance to Meloidogyne incognita in Avena sativa

Histology of Sclerotium cepivorum infection of onion roots and the spatial relationships of pectinases in the infection process

Anatomical, biochemical and transcriptome analyses of Vitis vinifera cv. 'Hongju' reveal novel information regarding the seed hardness of stenospermocarpic soft‐seed grapes

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