In situ cross-linked and highly carboxylated poly(vinyl alcohol) nanofibrous membranes for efficient adsorption of proteins

Abstract

Creating adsorptive materials for fast, efficient, and high-throughput adsorption and purification of proteins is critical to meet the great demands for highly purified proteins, yet has proven to be an highly challenging task. Here, we report that the cross-linked and highly carboxylated poly(vinyl alcohol) (PVA) nanofibrous membranes were fabricated by the combination of electrospinning and in-situ graft polymerization of PVA and maleic anhydride (MAH) under mild conditions. Taking advantages of the large surface area available for proteins binding, highly tortuous porous structure, and robust mechanical properties, the resultant PVA/MAH nanofibrous membranes exhibited integrated adsorption performance towards lysozyme involving superior adsorption capacity of 177 mg g -1 , fast adsorption equilibrium within 4 h, good selectivity, and good reversibility. Moreover, the saturation dynamic adsorption amount towards lysozyme reached 159 mg g -1 under 750 Pa driven solely by gravity, which was conformance to specify requirements for high adsorption capacity under relatively low pressure-drops. Furthermore, the adsorption performance towards proteins mixture was analyzed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and the resultant PVA/MAH nanofibrous membranes remained excellent stability under depyrogenation conditions. The successful fabrication of such fascinating nanofibrous materials by using this simple and intriguing approach may provide new insights into the design and development of adsorptive materials for purification proteins with superior adsorption performance.

Highly Carboxylated, Cellular Structured, and Underwater Superelastic Nanofibrous Aerogels for Efficient Protein Separation

“…Furthermore, as presented in the high‐magnification SEM images of the nanofibrous cell wall of IENFAs (Figure S9, Supporting Information), polymer coating layer could be obviously observed on the surface of SiO 2 nanofibers. The above chemical and physical structural characters could almost demonstrate the effective wrapping of carboxylated PVA functional layer on the SiO 2 nanofibers …”

Poly(Aspartic Acid) Functionalized Poly(ϵ-Caprolactone) Microspheres with Enhanced Hydroxyapatite Affinity as Bone Targeting Antibiotic Carriers

“…As many other antibiotics do not tolerate such extreme alkaline environments, our current motivation was to adjust the functionalization strategy by including functionalized cPVA surfactants in order to enrich the surface of the microspheres with COOH groups and omitting the harsh alkaline treatment procedure. While cPVA has been investigated for other applications (e.g., in relation with the modification of PVA crosslinking properties [ 31 , 32 ]), no reports have been found where cPVA was implemented as a surfactant for drug delivery purposes. Additionally, ASP oligomers were utilized as bone targeting moieties in this work opposed to ALN which showed impairment of osteoclast functionality in our previous work [ 18 ] and is associated with BRONJ pathology [ 24 ].…”

Functionalized Electrospun Poly(Vinyl Alcohol) Nanofibrous Membranes with Poly(Methyl Vinyl Ether-Alt-Maleic Anhydride) for Protein Adsorption

“…Among them, adsorption is of particular interest because of its cost-efficiency, time-efficiency, and ease in processing [ 3 ]. Traditionally, porous resin beads and gel microspheres have been used to capture selected species from protein mixtures [ 4 ]. The inherent problems of high pressure drops, high solution consumption, limited binding capacity, and low processing rates have restricted their use in large-scale applications [ 3 , 5 ].…”