In situ bioprinting – Bioprinting from benchside to bedside?

Singh, Satnam; Choudhury, Deepak; Yu, Fang; Mironov, Vladimir; Naing, May Win

doi:10.1016/j.actbio.2019.08.045

Cited by 192 publications

(151 citation statements)

References 69 publications

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“…Hydrogels can be fabricated via physical (such as hydrogen bonding and ionic and hydrophobic interactions) or chemical (such as photopolymerization or Michael-type addition reaction) crosslinking mechanisms [23]. Moreover, hydrogels can be engineered to exhibit adapted properties to the tissue to be repaired as 3D-bioprinted constructs [24,25] and/or injectable [26,27], stimuli-responsive [28,29], or adhesive systems [30,31].…”

Section: Hydrogels As Vehicles For Nonviral Gene Deliverymentioning

confidence: 99%

Hydrogel-Based Localized Nonviral Gene Delivery in Regenerative Medicine Approaches—An Overview

et al. 2020

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Hydrogel-based nonviral gene delivery constitutes a powerful strategy in various regenerative medicine scenarios, as those concerning the treatment of musculoskeletal, cardiovascular, or neural tissues disorders as well as wound healing. By a minimally invasive administration, these systems can provide a spatially and temporarily defined supply of specific gene sequences into the target tissue cells that are overexpressing or silencing the original gene, which can promote natural repairing mechanisms to achieve the desired effect. In the present work, we provide an overview of the most avant-garde approaches using various hydrogels systems for controlled delivery of therapeutic nucleic acid molecules in different regenerative medicine approaches.

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Section: Hydrogels As Vehicles For Nonviral Gene Deliverymentioning

confidence: 99%

Hydrogel-Based Localized Nonviral Gene Delivery in Regenerative Medicine Approaches—An Overview

et al. 2020

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“…37 Recently, in situ bioprinting provides direct printing in the defective site and produces patient-specific matrix through layer-by-layer printing the bio-inks under numerical system control. 59 Keriquel et al 60 used mesenchymal stromal cells (MSCs) nano-hydroxyapatite and collagen bio-ink for calvaria defect regeneration in the mice. The laser-assisted bioprinting was selected for in situ bioprinting of the prepared bio-ink in two different rings and disc geometrics.…”

Section: Bioprintingmentioning

confidence: 99%

“…Bioprinting is a promising technology for fabricating biological products, such as biological scaffolds, tissues, organs, and personalized medical devices through printing biological materials, living cells, and signaling molecules at the same time 37 . Recently, in situ bioprinting provides direct printing in the defective site and produces patient‐specific matrix through layer‐by‐layer printing the bio‐inks under numerical system control 59 . Keriquel et al 60 used mesenchymal stromal cells (MSCs) nano‐hydroxyapatite and collagen bio‐ink for calvaria defect regeneration in the mice.…”

Section: Bioprinting As An Innovative Technology For Bone Regenerationmentioning

confidence: 99%

Bioprinting a cell‐laden matrix for bone regeneration: A focused review

Ghorbani

Zhong

et al. 2020

J of Applied Polymer Sci

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Although many efforts have been made to regenerate the bone lesions, existing challenges can be mitigated through the development of tissue engineering scaffolds. However, the weak control on the microstructure of constructs, limitation in preparation of patient-specific and multilayered scaffolds, restriction in the fabrication of cell-laden matrixes, and challenges in preserving the drug/growth factors' efficacy in conventional methods have led to the development of bioprinting technology for regeneration of bone defects. So in this review, conventional 3D printers are classified, then the priority of the different types of bioprinting technologies for the preparation of the cell/growth factor-laden matrixes are focused. Besides, the bio-ink compositions, including polymeric/hybrid hydrogels and cell-based bio-inks are classified according to fundamental and recent studies. Herein, different effective parameters, such as viscosity, rheological properties, cross-linking methods, biodegradation biocompatibility, are considered. Finally, different types of cells and growth factors that can encapsulate in the bio-inks to promote bone repair are discussed, and both in vitro and in vivo achievement are considered. This review provides current and future perspectives of cell-laden bioprinting technologies. The restrictions and challenges are identified, and proper strategies for the development of cell-laden matrixes and high-performance printable bio-inks are proposed.

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“…The ionic/electrostatic interactions can instead achieve extremely limited mechanical strength. Moreover, the photo‐crosslinking process is the preferable choice to perform in situ bioprinting with robotic arms or handheld approaches, which is emerging as a favored bioprinting strategy during certain clinical situations when compared with conventional in vitro bioprinting. Finally, the photo‐crosslinking strategy allows to a precise temporal and spatial control compatible with the time frame in theater for surgical operations.…”

Section: Photo‐crosslinkable Hydrogel Bioscaffolds For Articular Cartmentioning

confidence: 99%

Human articular cartilage repair: Sources and detection of cytotoxicity and genotoxicity in photo-crosslinkable hydrogel bioscaffolds

Lee

O’Connell

Onofrillo

et al. 2019

Stem Cells Translational Medicine

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Three‐dimensional biofabrication using photo‐crosslinkable hydrogel bioscaffolds has the potential to revolutionize the need for transplants and implants in joints, with articular cartilage being an early target tissue. However, to successfully translate these approaches to clinical practice, several barriers must be overcome. In particular, the photo‐crosslinking process may impact on cell viability and DNA integrity, and consequently on chondrogenic differentiation. In this review, we primarily explore the specific sources of cellular cytotoxicity and genotoxicity inherent to the photo‐crosslinking reaction, the methods to analyze cell death, cell metabolism, and DNA damage within the bioscaffolds, and the possible strategies to overcome these detrimental effects.

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In situ bioprinting – Bioprinting from benchside to bedside?

Cited by 192 publications

References 69 publications

Hydrogel-Based Localized Nonviral Gene Delivery in Regenerative Medicine Approaches—An Overview

Hydrogel-Based Localized Nonviral Gene Delivery in Regenerative Medicine Approaches—An Overview

Bioprinting a cell‐laden matrix for bone regeneration: A focused review

Human articular cartilage repair: Sources and detection of cytotoxicity and genotoxicity in photo-crosslinkable hydrogel bioscaffolds

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