In situ biomineralization by silkworm feeding with ion precursors for the improved mechanical properties of silk fiber

Guo, Zhenhu; Xie, Wensheng; Gao, Qin; Wang, Dan; Gao, Fei; Li, Shengrong; Zhao, Lingyun

doi:10.1016/j.ijbiomac.2017.12.029

Cited by 33 publications

(25 citation statements)

References 32 publications

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“…Hence, 1.25 wt% was chosen as the maximum content of CNDs in this study to save the additional costs of further scale production and avoid any adverse effect of excessive CNDs on silkworms and silks [23]. Similar to other modified silks from silkworms fed silver nanoparticle [29], threonine [30], or nanohydroxyapatite powers [31], the mechanical properties of CNDs-modified silks were more variable than those of control silk. This may be attributed to the effect of exogenous addition on silkworm's spinning behavior including spinning speed, which significantly determines the performance of silk [32].…”

Section: Mechanical Properties Of Cnds-modified Silksmentioning

confidence: 99%

Super-strong and Intrinsically Fluorescent Silkworm Silk from Carbon Nanodots Feeding

et al. 2019

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HIGHLIGHTS • Intrinsically, super-strong fluorescent silk was fabricated via feeding Bombyx mori silkworms with carbon nanodots. • The multi-functional silks showed no cytotoxicity to Schwann cells and exhibited great potential in bioimaging. • The reinforcing mechanism of multi-functional silks was proposed. ABSTRACT Fluorescent silk is fundamentally important for the development of future tissue engineering scaffolds. Despite great progress in the preparation of a variety of colored silks, fluorescent silk with enhanced mechanical properties has yet to be explored. In this study, we report on the fabrication of intrinsically super-strong fluorescent silk by feeding Bombyx mori silkworm carbon nanodots (CNDs). The CNDs were incorporated into silk fibroin, hindering the conformation transformation, confining crystallization, and inducing orientation of mesophase. The resultant silk exhibited super-strong mechanical properties with breaking strength of 521.9 ± 82.7 MPa and breaking elongation of 19.2 ± 4.3%, improvements of 55.1% and 53.6%, respectively, in comparison with regular silk. The CNDs-reinforced silk displayed intrinsic blue fluorescence when exposed to 405 nm laser and exhibited no cytotoxic effect on cells, suggesting that multi-functional silks would be potentially useful in bioimaging and other applications.

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Section: Mechanical Properties Of Cnds-modified Silksmentioning

confidence: 99%

Super-strong and Intrinsically Fluorescent Silkworm Silk from Carbon Nanodots Feeding

et al. 2019

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“…The secondary structure of silk fibroin includes β-sheets, random coils and α-helices [ 16 , 17 , 18 ], which greatly control the physical properties. For instance, the mechanical properties of silk fiber can be enhanced by a high content of β-sheet crystals [ 19 , 20 ]. The highly crosslinked silk fibril network structure through β-sheet crystals is believed to also cause the insolubility of the regenerated silk materials in water and many mild organic solvents [ 10 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Study of Silk-Based Magnetic Materials: Effect of Magnetic Particle Types on the Protein Structure and Biomaterial Properties

Xue

Lofland

2020

IJMS

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This study investigates combining the good biocompatibility and flexibility of silk protein with three types of widely used magnetic nanoparticles to comparatively explore their structures, properties and potential applications in the sustainability and biomaterial fields. The secondary structure of silk protein was quantitatively studied by infrared spectroscopy. It was found that magnetite (Fe3O4) and barium hexaferrite (BaFe12O19) can prohibit β-sheet crystal due to strong coordination bonding between Fe3+ ions and carboxylate ions on silk fibroin chains where cobalt particles showed minimal effect. This was confirmed by thermal analysis, where a high temperature degradation peak was found above 640 °C in both Fe3O4 and BaFe12O19 samples. This was consistent with the magnetization studies that indicated that part of the Fe in the Fe3O4 and BaFe12O19 was no longer magnetic in the composite, presumably forming new phases. All three types of magnetic composites films maintained high magnetization, showing potential applications in MRI imaging, tissue regeneration, magnetic hyperthermia and controlled drug delivery in the future.

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“…Directly feeding silkworms or spiders artificial additives can be a practical way to produce reinforced silk fibers (102,103,(162)(163)(164)(165)(166). In this approach, silkworms can easily produce silk containing functional additive nanomaterials.…”

Section: Direct Feeding Of Artificial Additivesmentioning

confidence: 99%

“…Direct feeding of silkworms or spiders with functional nanomaterials in order to produce reinforced silk has been demonstrated with carbon-containing materials (e.g., graphene and CNTs) (102,(162)(163)(164), titanium dioxide (103), metal nanoparticles (e.g., silver and copper) (165), and ion precursors (e.g., Ca 2+ and PO 4 3− ) (166). Table 4 summarizes the mechanical properties of silk fibers from silkworms or spiders fed various artificial diets containing nanomaterials.…”

Section: Mechanically Enhanced Silkmentioning

confidence: 99%

Transgenic and Diet-Enhanced Silk Production for Reinforced Biomaterials: A Metamaterial Perspective

Leem

Fraser

Kim

2020

Annu. Rev. Biomed. Eng.

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Silk fibers, which are protein-based biopolymers produced by spiders and silkworms, are fascinating biomaterials that have been extensively studied for numerous biomedical applications. Silk fibers often have remarkable physical and biological properties that typical synthetic materials do not exhibit. These attributes have prompted a wide variety of silk research, including genetic engineering, biotechnological synthesis, and bioinspired fiber spinning, to produce silk proteins on a large scale and to further enhance their properties. In this review, we describe the basic properties of spider silk and silkworm silk and the important production methods for silk proteins. We discuss recent advances in reinforced silk using silkworm transgenesis and functional additive diets with a focus on biomedical applications. We also explain that reinforced silk has an analogy with metamaterials such that user-designed atypical responses can be engineered beyond what naturally occurring materials offer. These insights into reinforced silk can guide better engineering of superior synthetic biomaterials and lead to discoveries of unexplored biological and medical applications of silk.

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In situ biomineralization by silkworm feeding with ion precursors for the improved mechanical properties of silk fiber

Cited by 33 publications

References 32 publications

Super-strong and Intrinsically Fluorescent Silkworm Silk from Carbon Nanodots Feeding

Super-strong and Intrinsically Fluorescent Silkworm Silk from Carbon Nanodots Feeding

Comparative Study of Silk-Based Magnetic Materials: Effect of Magnetic Particle Types on the Protein Structure and Biomaterial Properties

Transgenic and Diet-Enhanced Silk Production for Reinforced Biomaterials: A Metamaterial Perspective

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