In-situ biofilm characterization in membrane systems using Optical Coherence Tomography: Formation, structure, detachment and impact of flux change

Dreszer, C.; Wexler, Adam D.; Drusová, Sandra; Overdijk, T.; Zwijnenburg, A.; Flemming, Hans Curt; Kruithof, Joop C.; Vrouwenvelder, Johannes S.

doi:10.1016/j.watres.2014.09.006

Cited by 152 publications

(89 citation statements)

References 50 publications

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“…OCT is a technique that allows the acquisition of three‐dimensional images from optical scattering media at a micrometer resolution 49, 50. By combining such an optical technique with electrochemical analyses, quantitative data with high temporal resolution can be obtained.…”

Section: Introductionmentioning

confidence: 99%

In situ Biofilm Quantification in Bioelectrochemical Systems by using Optical Coherence Tomography

Molenaar

Sleutels²,

Pereirã³

et al. 2018

ChemSusChem

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Detailed studies of microbial growth in bioelectrochemical systems (BESs) are required for their suitable design and operation. Here, we report the use of optical coherence tomography (OCT) as a tool for in situ and noninvasive quantification of biofilm growth on electrodes (bioanodes). An experimental platform is designed and described in which transparent electrodes are used to allow real‐time, 3D biofilm imaging. The accuracy and precision of the developed method is assessed by relating the OCT results to well‐established standards for biofilm quantification (chemical oxygen demand (COD) and total N content) and show high correspondence to these standards. Biofilm thickness observed by OCT ranged between 3 and 90 μm for experimental durations ranging from 1 to 24 days. This translated to growth yields between 38 and 42 mgCODbiomass gCODacetate −1 at an anode potential of −0.35 V versus Ag/AgCl. Time‐lapse observations of an experimental run performed in duplicate show high reproducibility in obtained microbial growth yield by the developed method. As such, we identify OCT as a powerful tool for conducting in‐depth characterizations of microbial growth dynamics in BESs. Additionally, the presented platform allows concomitant application of this method with various optical and electrochemical techniques.

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Section: Introductionmentioning

confidence: 99%

In situ Biofilm Quantification in Bioelectrochemical Systems by using Optical Coherence Tomography

Molenaar

Sleutels²,

Pereirã³

et al. 2018

ChemSusChem

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“…This approach allows for the direct observation of the dynamic deformations of the biofilm surface due to shearstress [16]. This is important for the study of biofouling processes, e.g., for the production of drinking water [11]. For the current study, we have chosen a relatively simple geometry of the flow channel.…”

Section: Discussionmentioning

confidence: 99%

“…In these experiments mixed bacterial communities are used instead of monocultures. D-glucose, sodium nitrate, and anhydrous sodium phosphate were used as nutrients and were added to tap water in a mass ratio carbon:nitrogen:phosphorous of 100:20:10 [11,24]. The initial The Reynolds number in the reference flow measurements is Re = 3.…”

Section: Flow Systemmentioning

confidence: 99%

“…Moreover, OCT is very well apt to measure local flow as Doppler-OCT has been applied to measure shear-rate distributions in asymmetric flow channels [10]. For biofilms, OCT imaging has been applied to study membrane biofouling [11][12][13], to study biofouling mitigation using two phase flow cleaning [14], to characterize biofilm structure in lab scale reactors [15], to study dynamic deformations and to estimate biofilm mechanical properties [16], to visualize biofilm formation in-vivo [17,18], to study structural transient effects [19,20], and to visualize biofilm growth under laminar and turbulent flow conditions [21].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Measurement of biofilm growth and local hydrodynamics using optical coherence tomography

Weiss¹,

Obied²,

Kalkman³

et al. 2016

Biomed. Opt. Express

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Abstract:We report on localized and simultaneous measurement of biofilm growth and local hydrodynamics in a microfluidic channel using optical coherence tomography. We measure independently with high spatio-temporal resolution the longitudinal flow velocity component parallel to the imaging beam and the transverse flow velocity component perpendicular to the imaging beam. Based on the measured velocities we calculate the shear-rates in the flow channel. We show the relation between the measured biofilm structure and flow velocities as biofilm growth progresses over the course of 48 hours. Vrouwenvelder, "In-situ biofilm characterization in membrane systems using optical coherence tomography: Formation, structure, detachment and impact of flux change," Water Res. 67, 243-254 (2014 Fane, "Gravity-driven membrane filtration as pretreatment for seawater reverse osmosis: Linking biofouling layer morphology with flux stabilization," Water Res. 70, 158-173 (2015). 13. S. West, M. Wagner, C. Engelke, and H. Horn, "Optical coherence tomography for the in situ three-dimensional visualization and quantification of feed spacer channel fouling in reverse osmosis membrane modules," J. Membrane

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“…The MFS has shown to be a suitable tool for prediction and characterization of membrane fouling [28][29][30]. Membrane sheets were cut from an unused commercial RO membrane, TFC-HR, manufactured by Koch Membrane Systems (USA).…”

Section: Long-term Biofouling Experimentsmentioning

confidence: 99%

Coating of reverse osmosis membranes with amphiphilic copolymers for biofouling control

Bucs¹,

Linares²,

Siddiqui³

et al. 2017

Desalination and Water Treatment

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a b s t r a c tSurface coating of membranes may be a promising option to control biofilm development and biofouling impact on membrane performance of spiral-wound reverse osmosis (RO) systems. The objective of this study was to investigate the impact of an amphiphilic copolymer coating on biofilm formation and biofouling control. The coating was composed of both hydrophilic and hydrophobic monomers hydroxyethyl methacrylate (HEMA) and perfluorodecyl acrylate (PFA), respectively. Commercial RO membranes were coated with HEMA-PFA copolymer film. Long and short term biofouling studies with coated and uncoated membranes and feed spacer were performed using membrane fouling simulators (MFSs) operated in parallel, fed with water containing nutrients. For the long-term studies pressure drop development in time was monitored and after eight days the MFSs were opened and the accumulated biofilm on the membrane and spacer sheets was quantified and characterized. The presence of the membrane coating was determined using X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR). Results showed that the amphiphilic coating (i) delayed biofouling (a lower pressure drop increase by a factor of 3 and a lower accumulated active biomass amount by a factor of 6), (ii) influenced the biofilm composition (23% lower polysaccharides and 132% higher protein content) and (iii) was still completely present on the membrane at the end of the biofouling study, showing that the coating was strongly attached to the membrane surface. Using coated membranes and feed spacers in combination with advanced cleaning strategies may be a suitable way to control biofouling.

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In-situ biofilm characterization in membrane systems using Optical Coherence Tomography: Formation, structure, detachment and impact of flux change

Cited by 152 publications

References 50 publications

In situ Biofilm Quantification in Bioelectrochemical Systems by using Optical Coherence Tomography

In situ Biofilm Quantification in Bioelectrochemical Systems by using Optical Coherence Tomography

Measurement of biofilm growth and local hydrodynamics using optical coherence tomography

Coating of reverse osmosis membranes with amphiphilic copolymers for biofouling control

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