In-Situ Biofabrication of Silver Nanoparticles in Ceiba pentandra Natural Fiber Using Entada spiralis Extract with Their Antibacterial and Catalytic Dye Reduction Properties

Abstract

It is believed of great interest to incorporate silver nanoparticles (Ag-NPs) into stable supported materials using biological methods to control the adverse properties of nanoscale particles. In this study, in-situ biofabrication of Ag-NPs using Entada spiralis (E. spiralis) aqueous extract in Ceiba pentandra (C. pentandra) fiber as supporting material was used in which, the E. spiralis extract acted as both reducing and stabilizing agents to incorporate Ag-NPs in the C. pentandra fiber. The properties of Ag-NPs incorporated in the C. pentandra fiber (C. pentandra/Ag-NPs) were characterized using UV-visible spectroscopy (UV-vis), X-ray Diffraction (XRD), Field Emission Transmission Electron Microscope (FETEM), Scanning Electron Microscope (Scanning Electron Microscope (SEM), Energy Dispersive X-ray (EDX), Brunauer-Emmett-Teller (BET), Thermogravimetric (TGA) and Fourier Transform Infrared (FTIR) analyses. The average size of Ag-NPs measured using FETEM image was 4.74 nm spherical in shape. The C. pentandra/Ag-NPs was easily separated after application, and could control the release of Ag-NPs to the environment due to its strong attachment in C. pentandra fiber. The C. pentandra/Ag-NPs exposed good qualitative and quantitative antibacterial activities against Staphylococcus aureus (ATCC 25923), Enterococcus faecalis (ATCC 29212), Escherichia coli (ATCC 25922) and Proteus vulgaris (ATCC 33420). The dye catalytic properties of C. pentandra/Ag-NPs revealed the dye reduction time in which it was completed within 4 min for 20 mg/L rhodamine B and 20 min for 20 mg/L methylene blue dye, respectively. Based on the results, it is evident that C. pentandra/Ag-NPs are potentially promising to be applied in wound healing, textile, wastewater treatment, food packaging, labeling and biomedical fields.

Exploiting Fruit Waste Grape Pomace for Silver Nanoparticles Synthesis, Assessing Their Antioxidant, Antidiabetic Potential and Antibacterial Activity Against Human Pathogens: A Novel Approach

“…The distinction in affectability of Gram-positive and Gram-negative microorganisms to GPE-AgNPs was because of the thickness and the constituents of their membrane structure [33]. The obtained results are found similar with the earlier studies concerning AgNPs synthesized using biological materials [9,52].…”

“…The surface plasmon resonance property of the GPE-AgNPs was confirmed by recording the absorbance spectra in the range of 300-650 nm at fixed time intervals by UV-Visible spectrophotometer, Optizen, Model-2120. Crystallinity and phase of the AgNPs were characterized by X-ray diffractometer (Rigaku, Tokyo, Japan) [9]. Fourier-transform infrared (FTIR) spectra of the GPE and synthesized GPE-AgNPs were recorded by taking spectra in the range of 400-4000 cm −1 on Fourier-transform infrared spectrophotometer (Perkin-Elmer, Norwalk, CT, USA) to identify the organic functional groups present on synthesized AgNPs.…”

“…Antioxidative potential of GPE, and synthesized GPE-AgNPs was evaluated by assessing their potential to scavenge the stable free radicals like 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-Azino-bis(3-ethylbenzothiazoline)-6 sulfonic acid (ABTS), taking standard as ascorbic acid. The procedure of both the enzyme assays and the DPPH and ABTS scavenging potential were determined using the formulas reported earlier [9]. For both enzyme activities, average mean values and standard deviation values were measured to calculate the antioxidant activity of GPE-AgNPs.…”

“…ROS are liable for insulin resistance, β-cell dysfunction, and also for both microvascular and macrovascular long-term complications of diabetes [22]. Green synthesized AgNPs fabricated with phytochemicals showed significant in vitro antioxidant potential, but yet their medical importance has not been scrutinized well [9,23].…”

“…Several routes of green synthesis of AgNPs by employing various biological materials such as microorganisms, whole plants, extracts of different parts of plants, fruit waste, and marine algae have been well investigated. Among which, utilization of plant- or fruit waste-based extracts could be more beneficial due to simple, cost-effective, and eco-accommodating process relative to other biological routes of NPs synthesis [ 8 , 9 ]. There are a few reports of green synthesis of silver nanoparticles using leaf extract of Holoptelea integrifolia , Punica granatum , waste fruit extract of Vitis vinifera , and using single biomolecules such as gallic acid, chlorotetracycline, and lignin [ 1 , 2 , 3 , 4 , 5 , 8 , 9 , 10 , 11 , 12 , 13 ].…”