In situ-Auswertung von Dünnschicht-Chromatogrammen über die Kubelka-Munk-Funktion

Ebel, S.; Hocke, J.

doi:10.1007/bf00602077

Cited by 5 publications

(1 citation statement)

References 4 publications

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“…Quantitative evaluation of spots on the developed TLC plates was done using a Zeiss PMQ II (18) and Zeiss PMQ III (21) spectrometer, Shimadzu CS-9000 densitometer (10). Ebel and Hocke (41) and Ebel (42), explained the basic differences between quantitative determinations done using liquid column chromatography and planar chromatography. The latter one uses the Kubelka -Munk equation (43,44) except when continuous development is applied.…”

Section: Monitoringmentioning

confidence: 99%

Forced-Flow Planar Chromatography in the Rear View Mirror

Kalász

2015

Journal of Chromatographic Science

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Mobile phase progress in planar stationary phase can be evoked by either external or internal forces. An internal force is capillarity, while gravity, electric field, a pump and centrifugal forces belong to external forces. Overpressured layer chromatography gives a widely used special chapter of forced-flow planar chromatography, a special bridge between high-performance liquid column chromatography and thin-layer chromatography (TLC). A simple and special rule characterizes the progress of mobile phase. Optimal efficiency is composed by the doubled effect of flow resulting from the pump-forced mobile phase (convex profile of laminar flow) and capillary forces on the dry stationary phase (concave laminar flow). This review describes the most important aspects of forced-flow TLC, including how the set-ups are developed and also the progress of detection methods used.

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