Calibration in TLC/HPTLC using the Michaelis-Menten function

Ebel, S.; Alert, D.; Schaefer, Uli

doi:10.1007/bf02279459

Cited by 13 publications

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References 15 publications

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“…The optical noise as defined by expressions ( 8 ) or (8a) is generally the most important factor which limits sensitivity and accuracy of densitometric measurements. In the interest of good performance it i s , therefore desirable to reduce it as much as practical, if possible to the level of electronic noise, which is the ultimate limitation.…”

Section: Another Source Of Error Which Affects Integration Is Determimentioning

confidence: 99%

Integration Errors in the Quantitative Evaluation of Flat Bed Separations

Pollak

1988

Journal of Liquid Chromatography

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Journal of Liquid ChromatographyPublication details, including instructions for authors and subscription information: ABSTRACT The paper discusses sources of errors which affect the accuracy of optical densitometry for the quantitative evaluation of TL chromatograms and flat bed electropherograms. Both 1D and 2D separations are considered. Relevant error sources fall into two classes. One which is deterministic in nature and one which has stochastic character. The main source of deterministic errors is the non-linear relation between densitometric response and concentration distribution. Linearizing transforms of the reponse signal are available which significantly reduce the magnitude of the error. They are most effective when ('point" scanning is used but much less so with slit scanning. stochastic sources produce noise which affects the measured value of the signal arnplitude. Most important is generally the "optical" noise of the blank medium.In most cases it is this factor, which ultimately determines sensitivity and accuracy of the method. Efforts to reduce optical noise are, therefore, an important design feature for high performance instrument. Most effective in this regard is two wave lengths scanning. The technique is, however, applicable only if the response characteristic of the medium is spectrally flat over a range which is significantly wider than the spectral absorbance characteristic of the separate. 1403

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Section: Another Source Of Error Which Affects Integration Is Determimentioning

confidence: 99%

Integration Errors in the Quantitative Evaluation of Flat Bed Separations

Pollak

1988

Journal of Liquid Chromatography

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High‐Performance Thin‐Layer Chromatography

Cebolla

Membrado

Jarne

et al. 2015

Analytical Separation Science

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The instrumental modularity of its basic steps, that is, sample application, chromatographic development, and detection, confers HPTLC a high degree of flexibility. Automated and computer‐controlled instruments with different levels of sophistication for each of these steps have been developed, for example, spray‐on sample applicators and chambers including the possibility of gradient separation. Detection techniques such as scanning densitometers, electronic documentation systems, bioactivity detection methodologies, and online coupling with mass spectrometry have come to be easily and firmly established in laboratories. Particularly, the introduction of an elution‐based TLC – MS interface has not only allowed original and efficient hyphenated systems to be designed but also opened the possibility of expanding the connection with other detectors. Thanks to this instrumental development, HPTLC has become a reliable, accurate, and sensitive analytical technique.

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Analytische Methoden für die Stabilitätsprüfung von Metronidazol‐Infusionslösungen

Ebel

Ledermann

Mümmler

1990

Archiv der Pharmazie

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UV-spectroscopy, thin layer- and high pressure liquid-chromatography as well as voltammetry (polarography) are suggested and compared as methods for use in stability testing of solutions of metronidazol. If some conditions are fulfilled UV-spectroscopy is the method of highest precision. TLC/HPTLC has advantages compared to HPLC, for more polar degradation products are visible at the starting point, whereas in HPLC these substances may be irreversibly adsorbed on the columne. Combination of HPLC and voltammetric (polarographic) techniques may lead to the optimal solution of this analytical problem with respect to best accuracy and precision (reproducibility) as compared in this paper.

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Calibration in TLC/HPTLC using the Michaelis-Menten function

Cited by 13 publications

References 15 publications

Integration Errors in the Quantitative Evaluation of Flat Bed Separations

Integration Errors in the Quantitative Evaluation of Flat Bed Separations

High‐Performance Thin‐Layer Chromatography

Analytische Methoden für die Stabilitätsprüfung von Metronidazol‐Infusionslösungen

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