In Silicon Cloning and Analysis of a LACS Gene from Glycine Max (L.)

Abstract

Long chain acyl-coenzyme A synthetases (LACSs) activate free fatty acids to acyl-CoA thioesters, and play important roles in the biosynthesis and degradation of lipids. In this study, a Glycine max(L.) LACS gene, designated as GmLACS, has been isolated through in silicon cloning. The gene is 2,219 bp with an open reading frame (ORF) of 1,989 bp, which encodes a LACS with 662 amino acid residues, with the isoelectric point of 6.42 and the calculated molecular mass of 65.6 kDa. Sequence analysis showed that GmLACS possessed typical domains of LACSs. Real-time quantitative PCR data analysis suggested that GmLACS was hightly expressed in leaves and young pods.

A Peroxisomal Long-Chain Acyl-CoA Synthetase from Glycine max Involved in Lipid Degradation

“…One cDNA (AK245419), namely GmLACS , has been studied preliminarily [23]. Another cDNA (AK245622), namely GmACSL2 according to the nomenclature for ACS enzymes [41], was selected.…”

“…Arabidopsis AtLACS1 to AtLACS9 (AAM28868 to AAM28876) were used as queries to tblastn G. max database, and two full-length cDNAs (AK245419 and AK245622) with high sequence similarity were found. One cDNA (AK245419), namely GmLACS , has been studied preliminarily [23] . Another cDNA (AK245622), namely GmACSL2 according to the nomenclature for ACS enzymes [41] , was selected.…”

“…The growing demand for vegetable oil has focused research toward on the metabolism of lipids in soybean [22] . So we cloned GmLACS from Glycine max previously, which may relate to the lipids synthesis [23] . In the present study, we identified another soybean ACSL, called GmACSL2 .…”