Identification of genes possibly related to loss of quality in late-season ‘Hass’ avocados in Chile

García-Rojas, Miguel; Gudenschwager, Orianne; Defilippi, Bruno G.; González-Agüero, Mauricio

doi:10.1016/j.postharvbio.2012.05.007

Cited by 16 publications

(16 citation statements)

References 25 publications

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“…Three biological samples in duplicate of quantitative PCR experiments were performed for each sample as described in García-Rojas et al [57]. Briefly, the amplification reaction was carried out in a total volume of 20 μl containing 1 pmol of each primer, 5 mM MgCl 2 , 1 ml LightCycler™ DNA Master SYBR® Green I (Roche Diagnostics) and 100 ng of each cDNA analyzed.…”

Section: Methodsmentioning

confidence: 99%

Identification of two putative reference genes from grapevine suitable for gene expression analysis in berry and related tissues derived from RNA-Seq data

et al. 2013

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BackgroundData normalization is a key step in gene expression analysis by qPCR. Endogenous control genes are used to estimate variations and experimental errors occurring during sample preparation and expression measurements. However, the transcription level of the most commonly used reference genes can vary considerably in samples obtained from different individuals, tissues, developmental stages and under variable physiological conditions, resulting in a misinterpretation of the performance of the target gene(s). This issue has been scarcely approached in woody species such as grapevine.ResultsA statistical criterion was applied to select a sub-set of 19 candidate reference genes from a total of 242 non-differentially expressed (NDE) genes derived from a RNA-Seq experiment comprising ca. 500 million reads obtained from 14 table-grape genotypes sampled at four phenological stages. From the 19 candidate reference genes, VvAIG1 (AvrRpt2-induced gene) and VvTCPB (T-complex 1 beta-like protein) were found to be the most stable ones after comparing the complete set of genotypes and phenological stages studied. This result was further validated by qPCR and geNorm analyses.ConclusionsBased on the evidence presented in this work, we propose to use the grapevine genes VvAIG1 or VvTCPB or both as a reference tool to normalize RNA expression in qPCR assays or other quantitative method intended to measure gene expression in berries and other tissues of this fruit crop, sampled at different developmental stages and physiological conditions.

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Section: Methodsmentioning

confidence: 99%

Identification of two putative reference genes from grapevine suitable for gene expression analysis in berry and related tissues derived from RNA-Seq data

et al. 2013

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“…ese disorders may show up during cold storage, as observed in "Edranol" and "Fuerte" for RA storage or after reaching a ready-to-eat stage at room temperature, as shown in fruit stored under CA or treated with 1-MCP [31,32]. Chilling injury symptoms during cold storage manifest in several ways, such as skin browning, internal browning, vascular browning, and increased pathogen susceptibility [33], and their relative importance depends on avocado variety, storage time, environmental conditions, and growing conditions and maturity stage, among factors.…”

Section: External Damagementioning

confidence: 99%

Effects of 1-Methylcyclopropene and Controlled Atmosphere on Ethylene Synthesis and Quality Attributes of Avocado cvs. Edranol and Fuerte

Olivares

Alvarez

Véliz

et al. 2020

Journal of Food Quality

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Avocado production worldwide relies on several varieties, with “Hass” being the most commercialized; however, the available genotypes include a number of green-skin varieties with important roles in several countries. Because many technologies have already been developed in “Hass” avocado, the main objective of this study was to evaluate the effects of controlled atmosphere (CA) storage and 1-methylcyclopropene (1-MCP) application during long-term storage of “Edranol” and “Fuerte” avocados. Fruits of both varieties were harvested at two maturity stages: an early harvest close to 20–23% dry matter (DM) content and another after two months, with 22% and 32% DM content for Edranol and Fuerte, respectively. After harvest, the fruit was stored under the following conditions: (i) regular air storage (RA), (ii) CA with 4% O2 and 6% CO2, and (iii) 1-MCP applied at 300 ppm. Avocados were stored at 5°C and 85% relative humidity. Physiological and quality evaluations were performed immediately after 30 and 50 days; afterwards, the avocados were maintained at 20°C (shelf life) until they reached the ready-to-eat stage. Ethylene synthesis was assessed by measuring the transcript accumulation of the ACO and ACS genes. The two varieties showed distinct respiration and ethylene production rates during ripening, and fruit stored under CA or after application of 1-MCP showed lower respiration rates than fruit stored under RA, with the lowest rate in 1-MCP-treated avocados. ACS and ACO transcript levels were also lower under both conditions. CA and 1-MCP were very effective tools for extending storage life mainly by reducing the fruit softening rate and the incidence of pulp disorders in both varieties, and interestingly, these techniques did not severely affect the days to reach the ready-to-eat stage. Therefore, the use of CA and 1-MCP technologies in “Fuerte” and “Edranol” seems to be suitable for maintaining quality through 50 days of storage.

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“…Transcript abundance was assessed by qPCR with the LightCycler® 96 system from Roche (Roche Diagnostics, Mannheim, Germany), as described in [54]. Genespecific primers ( Table 2) were designed using the online software Primer3plus [55] and were synthesized by IDT (Integrated DNA Technologies).…”

Section: Real-time Qpcr Assaysmentioning

confidence: 99%

Transcriptomic study of pedicels from GA3-treated table grape genotypes with different susceptibility to berry drop reveals responses elicited in cell wall yield, primary growth and phenylpropanoids synthesis

et al. 2020

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Background: Gibberellins (GA 3 ) are the most sprayed growth regulator for table grape production worldwide, increasing berry size of seedless varieties through pericarp cell expansion. However, these treatments also exacerbate berry drop, which has a detrimental effect on the postharvest quality of commercialized clusters. Several studies have suggested that pedicel stiffening caused by GA 3 would have a role in this disorder. Nevertheless, transcriptional and phenotypic information regarding pedicel responses to GA 3 is minimal.Results: Characterization of responses to GA 3 treatments using the lines L23 and Thompson Seedless showed that the former was up to six times more susceptible to berry drop than the latter. GA 3 also increased the diameter and dry matter percentage of the pedicel on both genotypes. Induction of lignin biosynthesis-related genes by GA 3 has been reported, so the quantity of this polymer was measured. The acetyl bromide method detected a decreased concentration of lignin 7 days after GA 3 treatment, due to a higher cell wall yield of the isolated fractions of GA 3treated pedicel samples which caused a dilution effect. Thus, an initial enrichment of primary cell wall components in response to GA 3 was suggested, particularly in the L23 background. A transcriptomic profiling was performed to identify which genes were associated with these phenotypic changes. This analysis identified 1281 and 1787 genes differentially upregulated by GA 3 in L23 and cv. Thompson Seedless, respectively. Concomitantly, 1202 and 1317 downregulated genes were detected in L23 and cv. Thompson Seedless (FDR < 0.05). Gene ontology analysis of upregulated genes showed enrichment in pathways including phenylpropanoids, cell wall metabolism, xylem development, photosynthesis and the cell cycle at 7 days post GA 3 application. Twelve genes were characterized by qPCR and striking differences were observed between genotypes, mainly in genes related to cell wall synthesis. Conclusions: High levels of berry drop are related to an early strong response of primary cell wall synthesis in the pedicel promoted by GA 3 treatment. Genetic backgrounds can produce similar phenotypic responses to GA 3 , although there is considerable variation in the regulation of genes in terms of which are expressed, and the extent of transcript levels achieved within the same time frame.

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Identification of genes possibly related to loss of quality in late-season ‘Hass’ avocados in Chile

Cited by 16 publications

References 25 publications

Identification of two putative reference genes from grapevine suitable for gene expression analysis in berry and related tissues derived from RNA-Seq data

Identification of two putative reference genes from grapevine suitable for gene expression analysis in berry and related tissues derived from RNA-Seq data

Effects of 1-Methylcyclopropene and Controlled Atmosphere on Ethylene Synthesis and Quality Attributes of Avocado cvs. Edranol and Fuerte

Transcriptomic study of pedicels from GA3-treated table grape genotypes with different susceptibility to berry drop reveals responses elicited in cell wall yield, primary growth and phenylpropanoids synthesis

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