In-Silico Target Prediction for Hsa-Mir-27a and Identification of Genes Involved In Breast Cancer

Abstract

The aim was to evaluate the impact of ABCB1 polymorphisms, C1236T and G2677T/A on the incidence of depression, its progression and effectiveness of antidepressant treatment in Polish population. Analyses were performed in 92 patients with recurrent depressive disorder (rDD) using automated sequencing. The obtained data were correlated with the results for C3435T polymorphism published earlier. Following that, a haplotype analysis was performed. For G2677T/A, the severity of depressive symptoms was higher among patients with GG. The more severe course of disease, the more frequently the lack of A or T allele was observed. Moreover, better effectiveness of the therapy was indicated in individuals with 1236 CC as well as in 2677 GG. Additionally, association of C1236T and (C1236T-G2677T/A-C3435T) haplotype with depression development was observed. Simultaneously, G2677T/A was connected with the severity of depressive symptoms. Both of the examined polymorphisms have influence on the effectiveness of antidepressant medications.

Identification of Genes Crucial for Biological Processes in Breast Cancer Liver Metastasis Relapse

“…These targeted miRNAs are important regulators involved in the development and metastasis of breast cancer that are mainly associated with the change in expression of PCK1 and LPL. Additionally, has-miR-27a-3p is a two-sided regulator of the transformation of metabolism and metastasis [19]. Evidence has shown that it can reduce the expression of PCK1, which is an essential enzyme in gluconeogenesis, and shift the metabolism toward favoring cell proliferation and metastasis.…”

Integrated Bioinformatics Analysis for the Screening of Hub Genes in Breast Cancer Metastasis Recurrence at Liver

“…These targeted miRNA are important regulators involved in the development and metastasis of breast cancer that are mainly associated with the change in expression of PCK1 and LPL. Additionally, has-miR-27a-3p has been found to be a two-sided regulator of transformation of metabolism and metastasis [10]. Evidence has shown that it can reduce the expression of PCK1, which is an essential enzyme in gluconeogenesis and shift the metabolism toward favoring cell proliferation and metastasis.…”