In silico prediction of the G-protein coupled receptors expressed during the metamorphic molt of Sagmariasus verreauxi (Crustacea: Decapoda) by mining transcriptomic data: RNA-seq to repertoire

Buckley, Sean J.; Fitzgibbon, Quinn P.; Smith, Gregory G.; Ventura, Tomer

doi:10.1016/j.ygcen.2016.02.001

Cited by 56 publications

(56 citation statements)

References 163 publications

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“…Since then, a mixed tissue transcriptome sequencing projects have been performed from the spider crab, Hyas araneus [118, 119] and spiny lobster Sagmariasus verreauxi [120]. Some of the most proactive work in crustacean transcriptomics lies in the area of neuropeptidomics for the discovery of neuroactive peptides and their receptors [121–124], including a study that makes use of the H. americanus sequence data contained reported here [4]. However, none of these studies provides analysis of ion channel or receptor sequence as contained within our study.…”

Section: Discussionmentioning

confidence: 99%

Deep sequencing of transcriptomes from the nervous systems of two decapod crustaceans to characterize genes important for neural circuit function and modulation

et al. 2016

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BackgroundCrustaceans have been studied extensively as model systems for nervous system function from single neuron properties to behavior. However, lack of molecular sequence information and tools have slowed the adoption of these physiological systems as molecular model systems. In this study, we sequenced and performed de novo assembly for the nervous system transcriptomes of two decapod crustaceans: the Jonah crab (Cancer borealis) and the American lobster (Homarus americanus).ResultsForty-two thousand, seven hundred sixty-six and sixty thousand, two hundred seventy-three contigs were assembled from C. borealis and H. americanus respectively, representing 9,489 and 11,061 unique coding sequences. From these transcripts, genes associated with neural function were identified and manually curated to produce a characterization of multiple gene families important for nervous system function. This included genes for 34 distinct ion channel types, 17 biogenic amine and 5 GABA receptors, 28 major transmitter receptor subtypes including glutamate and acetylcholine receptors, and 6 gap junction proteins – the Innexins.ConclusionWith this resource, crustacean model systems are better poised for incorporation of modern genomic and molecular biology technologies to further enhance the interrogation of fundamentals of nervous system function.

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Section: Discussionmentioning

confidence: 99%

Deep sequencing of transcriptomes from the nervous systems of two decapod crustaceans to characterize genes important for neural circuit function and modulation

et al. 2016

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“…The lengthy transition, the large-sized larvae and their transparency enable clear molt staging by gut retraction. The availability of transcriptomic data for both the larval metamorphic transition at high resolution [35], alongside tissues of juveniles and adults from this species [44][45][46][47][48][49] enables thorough examination of expression patterns and correlation with spatial-temporal expression.…”

Section: Metamorphosis In Spiny Lobstersmentioning

confidence: 99%

CYP450s analysis across spiny lobster metamorphosis identifies a long sought missing link in crustacean development

Ventura

Bose

Fitzgibbon

et al. 2017

The Journal of Steroid Biochemistry and Molecular Biology

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, CYP450s analysis across spiny lobster metamorphosis identifies a long sought missing link in crustacean development, Journal of Steroid Biochemistry and Molecular Biologyhttp://dx.doi.org/10. 1016/j.jsbmb.2017.04.007 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. CYP450s analysis across spiny lobster metamorphosis identifies a long sought missing link in crustacean development

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“…However, the distributions of these receptors within the nervous system are not known. In a study in the spiny lobster Sagmariasus verreauxi , Buckley et al [36]* identified 85 G-protein coupled receptors (GPCRs). Although the transcriptomes used in this study were not nervous system specific, they were able to identify sequences for many expected neuropeptide receptors.…”

Section: Identity and Distribution Of Neuropeptide Receptorsmentioning

confidence: 99%

“…What is not clear is whether these differences are due to different binding affinities for the peptides on the same receptor or whether the peptides activate different receptors. Thus far, only one ( Homarus ; [1]*) or two ( Sagmariasus verreauxi ; [36]*) FMRFamide-like receptors have been predicted by transcriptomic studies.…”

Section: Comparative Physiological Studies Of Modulators Affecting Crmentioning

confidence: 99%

Neuropeptide modulation of pattern-generating systems in crustaceans: comparative studies and approaches

Dickinson

Stanhope

2016

Current Opinion in Neurobiology

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Central pattern generators are subject to modulation by peptides, allowing for flexibility in patterned output. Current techniques used to characterize peptides include mass spectrometry and transcriptomics. In recent years, hundreds of neuropeptides have been sequenced from crustaceans; mass spectrometry has been used to identify peptides and to determine their levels and locations, setting the stage for comparative studies investigating the physiological roles of peptides. Such studies suggest that there is some evolutionary conservation of function, but also divergence of function even within a species. With current baseline data, it should be possible to begin using comparative approaches to ask fundamental questions about why peptides are encoded the way that they are and how this affects nervous system function.

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In silico prediction of the G-protein coupled receptors expressed during the metamorphic molt of Sagmariasus verreauxi (Crustacea: Decapoda) by mining transcriptomic data: RNA-seq to repertoire

Cited by 56 publications

References 163 publications

Deep sequencing of transcriptomes from the nervous systems of two decapod crustaceans to characterize genes important for neural circuit function and modulation

Deep sequencing of transcriptomes from the nervous systems of two decapod crustaceans to characterize genes important for neural circuit function and modulation

CYP450s analysis across spiny lobster metamorphosis identifies a long sought missing link in crustacean development

Neuropeptide modulation of pattern-generating systems in crustaceans: comparative studies and approaches

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