In silico, in vitro screening of antioxidant and anticancer potentials of bioactive secondary metabolites from an endophytic fungus (Curvularia sp.) from Phyllanthus niruri L

Kalimuthu, Arjun Kumar; Pavadai, Parasuraman; Pandian, Sivakumar; Murugesan, Sankaranarayanan; Arunachalam, Sankarganesh; Pandian, Sureshbabu Ram Kumar; Ravishankar, Vigneshwaran; Ammunje, Damodar Nayak; Sampath, Muthukumar; Panneerselvam, Theivendran; Kunjiappan, Selvaraj

doi:10.1007/s11356-022-19249-0

Cited by 21 publications

(14 citation statements)

References 79 publications

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“…It is comprised of species associated with plant and human as pathogens(Khiralla et al 2019). However, its species have also been isolated as endophytes from various plants(Khiralla et al 2016;Sharma et al 2018;Kalimuthu et al 2022). A previous study conducted byKharwar et al (2011) reported endophytic Curvularia lunata isolated from leaf tissue of Adenocalymma alliaceum as dominant species which well corroborates the present study.…”

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confidence: 91%

Diversity, community composition and phylogeny of culturable fungal endophytes of Dioscorea bulbifera L.

Sharma

Dhar

Kaul

2022

Preprint

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The origin of endophytic symbiosis dates long back in evolutionary history. Over the period of evolution, they have established a special relationship with their host plants. The research nowadays is focused on understanding the ecology, evolution and bioactive potential of endophytes. The current study is aimed to describe the biodiversity of culturable fungal endophytes associated with Dioscorea bulbifera L. Overall, the endophytic fungal taxa belonged to two phyla, four classes, ten orders, twenty-five genera and thirty-eight species showing abundant diversity. The class Dothidiomycetes was reported to be the richest and most abundant in terms of species richness. Diversity indices demonstrated significant diversity of fungal endophytes within and between the different tissues. Moreover, fungal isolates such as, Acrocalymma medicaginis, Curvularia lycoperscii, Talaromyces macrospora, Fusarium laceratum, Paecilomyces formosus and Microascus cirrosus obtained in this study have not been reported earlier as endophytes from any plant. The endophytic fungal isolates, Nigrospora oryzae, Flavodon flavus and Talaromyces sp., have been reported first time as endophytes from D. bulbifera L. Since, the relevance of endophyte-plant interactions is still indistinct therefore, deeper understanding of these interactions at molecular and genetic level is required to be done.

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confidence: 91%

Diversity, community composition and phylogeny of culturable fungal endophytes of Dioscorea bulbifera L.

Sharma

Dhar

Kaul

2022

Preprint

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“…Futher, GC-MS analysis confirmed the presence of 2,4-di-tert-butylphenol, heptadecane, 2,6,10,14-tetramethyl, tetradecanoic acid, 2-hydroxy-, methyl ester, γ-terpinene, p-cymene, and oxirane (chloromethyl) in the crude extract of the fungus (Nwobodo et al, 2022 ). An in vitro study was done by Kalimuthu et al ( 2022 ) that conclude the anti-oxidant and anti-cancer potential of Curvularia geniculata isolated from Phyllanthus niruri L. They extract total 13 compounds from which 2-methyl-7-phenylindole shows high binding affinity for epidermal growth factor receptor (EGFR), having high radical scavenging activity and cytotoxicity against HepG2 cell lines with IC 50 value of 62.23 μg/mL. Curvularia species produce a vast diversity of secondary metabolites in which polyketides, alkaloids, quinones, and terpenes are major groups.…”

Section: Diversity Of Secondary Metabolites Of Curvularia ...mentioning

confidence: 99%

Bioactive compounds of Curvularia species as a source of various biological activities and biotechnological applications

2022

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Many filamentous fungi are known to produce several secondary metabolites or bioactive compounds during their growth and reproduction with sort of various biological activities. Genus Curvularia (Pleosporaceae) is a dematiaceous filamentous fungus that exhibits a facultative pathogenic and endophytic lifestyle. It contains ~213 species among which Curvularia lunata, C. geniculata, C. clavata, C. pallescens, and C. andropogonis are well-known. Among them, C. lunata is a major pathogenic species of various economical important crops especially cereals of tropical regions while other species like C. geniculata is of endophytic nature with numerous bioactive compounds. Curvularia species contain several diverse groups of secondary metabolites including alkaloids, terpenes, polyketides, and quinones. Which possess various biological activities including anti-cancer, anti-inflammatory, anti-microbial, anti-oxidant, and phytotoxicity. Several genes and gene factors are involved to carry and regulate the expression of these activities which are influenced by environmental signals. Some species of Curvularia also show negative impacts on humans and animals. Apart from their negative effects, there are some beneficial implications like production of enzymes of industrial value, bioherbicides, and source of nanoparticles is reported. Many researchers are working on these aspects all over the world but there is no review in literature which provides significant understanding about these all aspects. Thus, this review will provide significant information about secondary metabolic diversity, their biological activities and biotechnological implications of Curvularia species.

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“…The DPPH radical scavenging activity of H. indicus (L) R.Br. extract was performed using DPPH reagent (4g DPPH dissolved in 90% methanol), as indicated by our previously published article with slight modi cation (47). Brie y, 0.1 mM DPPH solution (0.1 mM) 1 mL of freshly prepared DPPH solution was added to 3 mL of different concentrations (6.25-100 mg mL-1) of H. indicus (L) R.Br.…”

Section: Dpph Radical Scavenging Activitymentioning

confidence: 99%

Pharmacoinformatics-based prediction of potential Keap1 inhibitors from Hemidesmus indicus (L) R.Br. against oxidative stress-induced diseases

Vellur

Pavadai²,

Palanichamy

et al. 2022

Preprint

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The Keap1-Nrf2 pathway plays a prominent role in activating cytoprotective genes, detoxification and antioxidative defense enzymes against oxidative stress and xenobiotics-induced damage. Oxidative stress is involved in the initiation and progression of numerous health complications. The present study investigated the antioxidant potential of aqueous methanolic extract of Hemidesmus indicus (L) R.Br., followed by a pharmacoinformatics-based screening of novel Keap1 protein inhibitors. Initially, the antioxidant potential of this target plant was assessed by antioxidant assays (DPPH, ABTS radical scavenging and FRAP). H. indicus (L) R.Br. extract (100 µg mL− 1) showed 85 ± 2.917%, 78.783 ± 0.24% of DPPH, ABTS radicals scavenging activity, and 161 ± 4 µg mol (Fe (II)) g− 1 ferric ion reducing power. Further, a total of sixty-nine phytocompounds were derived from this plant through the IMPPAT database, and their three-dimensional structures were obtained from the PubChem database. The chosen sixty-nine phytocompounds were docked against the Kelch-Neh2 complex protein (PDB entry ID: 2flu, resolution 1.50 Å) along with the standard drug (CPU192018). The top scored three hits were selected, namely Hemidescine (− 11.30 Kcal mol− 1), Beta-Amyrin (− 10.00 Kcal mol− 1), and Quercetin (− 9.80 Kcal mol− 1) based on their binding affinities. The selected three hits showed significant drug-likeness properties with the least toxicity profile. Molecular dynamics simulation studies showed that all the protein-ligand complexes (Keap1-HEM, Keap1-BET and Keap1-QUE) were highly stable during the entire simulation period, compared to standard CPUY192018-Keap1complex. Based on these findings, the top-scored three phytocompounds may be used as a significant and safe Keap1 inhibitor and could potentially use for oxidative stress-induced health complications.

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In silico, in vitro screening of antioxidant and anticancer potentials of bioactive secondary metabolites from an endophytic fungus (Curvularia sp.) from Phyllanthus niruri L

Cited by 21 publications

References 79 publications

Diversity, community composition and phylogeny of culturable fungal endophytes of Dioscorea bulbifera L.

Diversity, community composition and phylogeny of culturable fungal endophytes of Dioscorea bulbifera L.

Bioactive compounds of Curvularia species as a source of various biological activities and biotechnological applications

Pharmacoinformatics-based prediction of potential Keap1 inhibitors from Hemidesmus indicus (L) R.Br. against oxidative stress-induced diseases

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