In-silico identification of miRNAs and their regulating target functions in Ocimum basilicum

Identification of miRNAs and their targets involved in the secondary metabolic pathways of Mentha spp.

“…miR156 and miR414 showed regulation for nascent polypeptide-associated complex subunit alpha-like protein (NACA), accountable for stress response. The regulation of miR414 for the same target was earlier reported in O. basilicum (Singh and Sharma, 2014). Hence, the results also suggest the regulatory control of stress stimuli with the modulation of NACA by miR414 in Lamiaceae.…”

“…We have reported miR5015, miR5645, miR834, miR838, miR862 (5 new miRNA family) families for the first time from Lamiaceae. Rest two miRNAs families (miR414 and miR5021) were earlier reported by us from the plant Ocimum basilicum (Singh and Sharma, 2014). All predicted miRNAs showed a high percentage of sequence similarity with their respective miRNAs families (Fig.…”

Correlation analysis of the mRNA and miRNA expression profiles in the nascent synthetic allotetraploid Raphanobrassica

“…Based on the information contained in GO enrichment of mRNAs and miRNAs, the GO term ‘metabolic process’ was significantly enriched, no matter in differentially expressed mRNAs/miRNAs (target genes) or non-additively expressed mRNAs/miRNAs (target genes) in Raphanobrassica compared with its parents. MiRNA5658a, which contained 44.94% of miRNA-target pairs in correlation network in the present study, was also significantly enriched in the terms ‘protein catabolic process’, ‘response to stress’ and ‘response to biotic and abiotic stress’ in Ocimum basilicum 42. The 31 related target genes showing non-additive expression were also significantly enriched in ‘response to stress’ and response to biotic’ in the present study, but the non-additively expressed gene targets of miRNA5658a were significantly enriched in the ‘metabolic process’ category (Fig.…”

“…Non-additively expressed mRNAs and miRNAs in polyploidy have been detected in allotetraploid Arabidopsis , hexaploid wheat and citrus allopolyploids42942. In the present study, the RNA-seq technique were used to compare mRNA and miRNA expression profiles between Raphanobrassica and its parents, and a total of 9,209 (27.43%) genes showed expression divergence, while 5,219 (15.55%) genes were non-additively expressed in the tetraploid Raphanobrassica.…”

In silico identification of candidate microRNAs and their targets in potato somatic hybrid Solanum tuberosum (+) S. pinnatisectum for late blight resistance

“…The miR5021 participate in multiple biological and metabolic processes, including signal transduction, stress response, MYB transcription factors and plant development in Brassica oleracea (Wang et al, 2012a) and cotton (Wang et al, 2012b). It plays important role in plant development in amino acid (cysteine and methionine) metabolism in Ocimum basilicum (Singh and Sharma, 2014) and regulates ethylene and polyamines syntheses in Catharanthus roseus (Pani and Mahapatra, 2013). Being one of the largest miRNAs families in response to cold stress, it may regulate cellular, physiological or developmental processes specific to peach and/or other tree species (Barakat et al, 2012).…”